In outside-out patches, GlyR openings to the largest conductance are longer than those to lower conductance levels.
The top row of graphs (A, B) shows the dwell-time distributions for spinal GlyRs recorded in the outside-out configuration and constructed including all conductance levels.
The bottom two graphs show conditional open period distributions, plotted for openings to the largest conductance (greater than 4pA at −100 mV, panel D) or to the two smaller conductances (less than 4 pA, panel C). In panel D, the dashed line shows for reference the exponential fitted to the distribution in panel C (and vice-versa), scaled to contain the same number of events. The longer duration of the larger openings is very apparent.