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. Author manuscript; available in PMC: 2009 Mar 10.
Published in final edited form as: Int J Parasitol. 2007 May 10;37(12):1351–1358. doi: 10.1016/j.ijpara.2007.04.017

Fig. 3.

Fig. 3

Activity assays performed with extracts from the anterior midgut of Triatoma brasiliensis from the saline injected group (control), β-lactamase gene double-stranded RNA (dsRNA) injected group (BLA) and brasiliensin dsRNA injected group (Brasiliensin) extracted 48 h after the second injection (n = 6). (a) Thrombin activity assay. Extracts were assayed using 0.03 U human thrombin and the synthetic chromogenic substrate S2238 (0.125 mM). Results were expressed as the percentage of homogenate necessary to inhibit 50% of the enzyme. (b) Citrated plasma recalcification time assay. Coagulation time was defined as the time taken for the turbidity to achieve an absorbance reading of 0.025 absorbance units, the absorbance at zero time being taken as zero absorbance units. Asterisks indicate groups with significant statistical differences (P < 0.05). Results are represented as the average ± standard error.