Abstract
The degree of haemolysis (y) of SRC sensitized with horse haemolysin by fowl complement was measured. A linear relation was not found between log [y/(1—y)] and log x, the log volume of fowl complement used.
Absorption of fowl C′ in the cold with packed SRC did not always result in C′ free from lysins to SRC. Estimations on two C′ sera from which SRC lysins had been removed gave a straight line with an indicator system of SRC sensitized with `immune' fowl haemolysin.
Linear relations over a wide range of C′ concentrations, were also obtained when the indicator system consisted of SRC sensitized with a combination of `immune' and naturally occurring antibodies. The range could be extended by the addition of a small amount of heated normal fowl serum.
Full text
PDF






Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BENSON H. N., BRUMFIELD H. P., POMEROY B. S. Requirement of avian C'1 for fixation of guinea pig complement by avian antibody-antigen complexes. J Immunol. 1961 Nov;87:616–622. [PubMed] [Google Scholar]
- McVICKAR D. L. Enhancement of hemolytic activity of complement by polyethylene glycols. Proc Soc Exp Biol Med. 1949 Nov;72(2):384–384. doi: 10.3181/00379727-72-17440. [DOI] [PubMed] [Google Scholar]
- ORLANS E., ROSE M. E., CLAPP K. H. Fowl antibody: V. The interactions of fresh and heated fowl serum and of guinea-pig complement measured by the lysis of sensitized red cells. Immunology. 1962 Nov;5:649–655. [PMC free article] [PubMed] [Google Scholar]
- ROSE M. E., ORLANS E. Fowl antibody: III. Its haemolytic activity with complements of various species and some properties of fowl complement. Immunology. 1962 Nov;5:633–641. [PMC free article] [PubMed] [Google Scholar]
- TALMAGE D. W., FRETER G. G., TALIAFERRO W. H. Two antibodies of related specificity but different hemolytic efficiency separated by centrifugation. J Infect Dis. 1956 May-Jun;98(3):300–305. doi: 10.1093/infdis/98.3.300. [DOI] [PubMed] [Google Scholar]
