Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1992 Nov;90(2):194–198.

Immune response to hepatitis B virus surface antigen peptides during HBV infection.

A Mishra 1, H Durgapal 1, V Manivel 1, S K Acharya 1, K V Rao 1, S K Panda 1
PMCID: PMC1554618  PMID: 1424274

Abstract

Antibody responses of patients with acute (n = 73), fulminant (n = 30) and chronic (n = 51) hepatitis B virus (HBV) infection as well as recovered individuals (n = 7) were studied against three synthetic peptides, Pre-S1 amino acids (aa. 12-32), Pre-S2 amino acids (aa. 120-145), and S amino acids (aa. 124-147) of the envelope region (HBsAg). T cell blastogenic response was investigated in a proportion of the patients (27 acute, nine fulminant, 13 chronic hepatitis and seven recovered individuals) along with seven HBV vaccinated and three normal individuals. The presence of T cell response against S peptide was observed in all the cases (9/9, 100%) during early acute hepatitis. This was suppressed during late stages (8/18, 44%) followed by partial reversal during recovery (5/7, 71%). T cell response and antibodies to Pre-S1 and Pre-S2 peptides were present only in one-third of the patients throughout these periods. The T cell blastogenic response as well as antibody reactivity against these peptides were absent and minimal in chronic hepatitis. Immune response against envelope protein appears to play a major role in acute hepatic injury due to HBV infection and help in virus clearance.

Full text

PDF
194

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldershvile J., Dietrichson O., Hardt F., Nielsen J. O., Skinhöj P. Humoral and cell-mediated immunity to hepatitis B virus antigens in acute and chronic liver disease. Scand J Gastroenterol. 1977;12(8):917–922. doi: 10.3109/00365527709181350. [DOI] [PubMed] [Google Scholar]
  2. Barnaba V., Levrero M., Van Dyke A. D., Musca A., Cordova C., Balsano F. T-cell subsets in the hyporesponsiveness to hepatitis B surface antigen (HBsAg) and antigen-specific suppressor lymphocytes in chronic hepatitis B virus (HBV) infection. Clin Immunol Immunopathol. 1985 Mar;34(3):284–295. doi: 10.1016/0090-1229(85)90177-1. [DOI] [PubMed] [Google Scholar]
  3. Beutner K. R., Tiku M. L., Ogra P. L. Hepatitis B surface antigen-specific cell-mediated immune responses in human chronic hepatitis B surface antigen carriers. Infect Immun. 1978 Aug;21(2):480–488. doi: 10.1128/iai.21.2.480-488.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bøyum A. Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol. 1976 Jun;Suppl 5:9–15. [PubMed] [Google Scholar]
  5. Celis E., Ou D., Otvos L., Jr Recognition of hepatitis B surface antigen by human T lymphocytes. Proliferative and cytotoxic responses to a major antigenic determinant defined by synthetic peptides. J Immunol. 1988 Mar 15;140(6):1808–1815. [PubMed] [Google Scholar]
  6. ELLMAN G. L. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959 May;82(1):70–77. doi: 10.1016/0003-9861(59)90090-6. [DOI] [PubMed] [Google Scholar]
  7. Ferrari C., Penna A., Bertoletti A., Valli A., Antoni A. D., Giuberti T., Cavalli A., Petit M. A., Fiaccadori F. Cellular immune response to hepatitis B virus-encoded antigens in acute and chronic hepatitis B virus infection. J Immunol. 1990 Nov 15;145(10):3442–3449. [PubMed] [Google Scholar]
  8. Ferrari C., Penna A., Sansoni P., Giuberti T., Neri T. M., Chisari F. V., Fiaccadori F. Selective sensitization of peripheral blood T lymphocytes to hepatitis B core antigen in patients with chronic active hepatitis type B. Clin Exp Immunol. 1986 Dec;66(3):497–506. [PMC free article] [PubMed] [Google Scholar]
  9. Ibrahim A. B., Vyas G. N., Perkins H. A. Immune response to hepatitis B surface antigen. Infect Immun. 1975 Jan;11(1):137–141. doi: 10.1128/iai.11.1.137-141.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Iwarson S., Tabor E., Thomas H. C., Goodall A., Waters J., Snoy P., Shih J. W., Gerety R. J. Neutralization of hepatitis B virus infectivity by a murine monoclonal antibody: an experimental study in the chimpanzee. J Med Virol. 1985 May;16(1):89–96. doi: 10.1002/jmv.1890160112. [DOI] [PubMed] [Google Scholar]
  11. Koszinowski U. H., Reddehase M. J., Jonjic S. The role of CD4 and CD8 T cells in viral infections. Curr Opin Immunol. 1991 Aug;3(4):471–475. doi: 10.1016/0952-7915(91)90005-l. [DOI] [PubMed] [Google Scholar]
  12. Manivel V., Ramesh R., Panda S. K., Rao K. V. A synthetic peptide spontaneously self-assembles to reconstruct a group-specific, conformational determinant of hepatitis B surface antigen. J Immunol. 1992 Jun 15;148(12):4006–4011. [PubMed] [Google Scholar]
  13. Milich D. R., Hughes J. L., McLachlan A., Thornton G. B., Moriarty A. Hepatitis B synthetic immunogen comprised of nucleocapsid T-cell sites and an envelope B-cell epitope. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1610–1614. doi: 10.1073/pnas.85.5.1610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Milich D. R., McLachlan A. The nucleocapsid of hepatitis B virus is both a T-cell-independent and a T-cell-dependent antigen. Science. 1986 Dec 12;234(4782):1398–1401. doi: 10.1126/science.3491425. [DOI] [PubMed] [Google Scholar]
  15. Milich D. R., McNamara M. K., McLachlan A., Thornton G. B., Chisari F. V. Distinct H-2-linked regulation of T-cell responses to the pre-S and S regions of the same hepatitis B surface antigen polypeptide allows circumvention of nonresponsiveness to the S region. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8168–8172. doi: 10.1073/pnas.82.23.8168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Milich D. R. Synthetic T and B cell recognition sites: implications for vaccine development. Adv Immunol. 1989;45:195–282. doi: 10.1016/s0065-2776(08)60694-x. [DOI] [PubMed] [Google Scholar]
  17. Mohite B. J., Rath S., Bal V., Kamat S. A., Marathe S. N., Zuckerman A. J., Kamat R. S. Mechanisms of liver cell damage in acute hepatitis B. J Med Virol. 1987 Jul;22(3):199–210. doi: 10.1002/jmv.1890220302. [DOI] [PubMed] [Google Scholar]
  18. Moriyama T., Guilhot S., Klopchin K., Moss B., Pinkert C. A., Palmiter R. D., Brinster R. L., Kanagawa O., Chisari F. V. Immunobiology and pathogenesis of hepatocellular injury in hepatitis B virus transgenic mice. Science. 1990 Apr 20;248(4953):361–364. doi: 10.1126/science.1691527. [DOI] [PubMed] [Google Scholar]
  19. Nagafuchi S., Kashiwagi S., Hayashi S., Inoue T., Imayama S., Takeshita M., Kikuchi M. Delayed type hypersensitivity (DTH) skin reaction to hepatitis B surface antigen (HBsAg) in patients with type B acute and chronic hepatitis. Clin Exp Immunol. 1985 Sep;61(3):569–576. [PMC free article] [PubMed] [Google Scholar]
  20. Scotto J., Hadchouel M., Hery C., Yvart J., Tiollais P., Brechot C. Detection of hepatitis B virus DNA in serum by a simple spot hybridization technique: comparison with results for other viral markers. Hepatology. 1983 May-Jun;3(3):279–284. doi: 10.1002/hep.1840030301. [DOI] [PubMed] [Google Scholar]
  21. Trey C., Lipworth L., Chalmers T. C., Davidson C. S., Gottlieb L. S., Popper H., Saunders S. J. Fulminant hepatic failure. Presumable contribution to halothane. N Engl J Med. 1968 Oct 10;279(15):798–801. doi: 10.1056/NEJM196810102791504. [DOI] [PubMed] [Google Scholar]
  22. Vento S., Hegarty J. E., Alberti A., O'Brien C. J., Alexander G. J., Eddleston A. L., Williams R. T lymphocyte sensitization to HBcAg and T cell-mediated unresponsiveness to HBsAg in hepatitis B virus-related chronic liver disease. Hepatology. 1985 Mar-Apr;5(2):192–197. doi: 10.1002/hep.1840050206. [DOI] [PubMed] [Google Scholar]
  23. Vento S., Rondanelli E. G., Ranieri S., O'Brien C. J., Williams R., Eddleston A. L. Prospective study of cellular immunity to hepatitis-B-virus antigens from the early incubation phase of acute hepatitis B. Lancet. 1987 Jul 18;2(8551):119–122. doi: 10.1016/s0140-6736(87)92329-4. [DOI] [PubMed] [Google Scholar]
  24. Yamauchi K., Nakanishi T., Chiou S. S., Obata H. Suppression of hepatitis B antibody synthesis by factor made by T cells from chronic hepatitis B carriers. Lancet. 1988 Feb 13;1(8581):324–326. doi: 10.1016/s0140-6736(88)91119-1. [DOI] [PubMed] [Google Scholar]
  25. de Moura M. C., Vernace S. J., Paronetto F. Cell-mediated immune reactivity to hepatitis B surface antigen in liver diseases. Gastroenterology. 1975 Aug;69(2):310–317. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES