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. 1992 Mar;58(3):948–952. doi: 10.1128/aem.58.3.948-952.1992

Induction of ploidy level increments in an asporogenous industrial strain of the yeast Saccharomyces cerevisiae by UV irradiation.

T Sasaki 1
PMCID: PMC195361  PMID: 1575498

Abstract

Cells of an asporogenous industrial strain of the yeast Saccharomyces cerevisiae were irradiated with UV light by using a method that was developed previously (T. Sasaki and Y. Ohshima, Appl. Environ. Microbiol. 53:1504-1511, 1987). This treatment gave rise to large-cell clones among the surviving cells, from which colonies consisting of cells with a normal morphology and a prototrophic property were obtained. The large-cell trait of these was stably inheritable, with the cell volumes being about twice that of the parent for 7 years on a slant agar medium at 4 degrees C with repeated transfers. The cellular DNA content of these clones, in comparison to those of two authentic haploid strains, was determined by chemical analysis. The ratio of the DNA contents showed that the parent and its large-cell derivatives were a diploid and tetraploids, respectively. No abnormality was found in the chromosomal DNA patterns of the large-cell clones, at least as determined by agarose gel electrophoresis with a CHEF-DR II pulsed-field electrophoresis system. These findings led to the conclusion that our UV light method is applicable for inducing ploidy level increments in the widely used yeast species S. cerevisiae.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bostock C. J. DNA synthesis in the fission yeast Schizosaccharomyces pombe. Exp Cell Res. 1970 Apr;60(1):16–26. doi: 10.1016/0014-4827(70)90484-2. [DOI] [PubMed] [Google Scholar]
  2. Herskowitz I. Life cycle of the budding yeast Saccharomyces cerevisiae. Microbiol Rev. 1988 Dec;52(4):536–553. doi: 10.1128/mr.52.4.536-553.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hirschman S. Z., Felsenfeld G. Determination of DNA composition and concentration by spectral analysis. J Mol Biol. 1966 Apr;16(2):347–358. doi: 10.1016/s0022-2836(66)80178-x. [DOI] [PubMed] [Google Scholar]
  4. Johnston G. C. Cell size and budding during starvation of the yeast Saccharomyces cerevisiae. J Bacteriol. 1977 Nov;132(2):738–739. doi: 10.1128/jb.132.2.738-739.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Johnston G. C., Ehrhardt C. W., Lorincz A., Carter B. L. Regulation of cell size in the yeast Saccharomyces cerevisiae. J Bacteriol. 1979 Jan;137(1):1–5. doi: 10.1128/jb.137.1.1-5.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Johnston G. C., Pringle J. R., Hartwell L. H. Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp Cell Res. 1977 Mar 1;105(1):79–98. doi: 10.1016/0014-4827(77)90154-9. [DOI] [PubMed] [Google Scholar]
  7. Ohshima Y., Sugaura T., Horita M., Sasaki T. Industrial Application of Artificially Induced Diploid Strains of Torulaspora delbrueckii. Appl Environ Microbiol. 1987 Jul;53(7):1512–1514. doi: 10.1128/aem.53.7.1512-1514.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Parry J. M., Sharp D., Tippins R. S., Parry E. M. Radiation-induced mitotic and meiotic aneuploidy in the yeast Saccharomyces cerevisiae. Mutat Res. 1979 Jun;61(1):37–55. doi: 10.1016/0027-5107(79)90005-8. [DOI] [PubMed] [Google Scholar]
  9. Sasaki T., Ohshima Y. Induction and Characterization of Artificial Diploids from the Haploid Yeast Torulaspora delbrueckii. Appl Environ Microbiol. 1987 Jul;53(7):1504–1511. doi: 10.1128/aem.53.7.1504-1511.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  11. Thompson C. T., Dvorak J. A. Quantitation of total DNA per cell in an exponentially growing population using the diphenylamine reaction and flow cytometry. Anal Biochem. 1989 Mar;177(2):353–357. doi: 10.1016/0003-2697(89)90065-1. [DOI] [PubMed] [Google Scholar]
  12. Tyson C. B., Lord P. G., Wheals A. E. Dependency of size of Saccharomyces cerevisiae cells on growth rate. J Bacteriol. 1979 Apr;138(1):92–98. doi: 10.1128/jb.138.1.92-98.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wheals A. E. Size control models of Saccharomyces cerevisiae cell proliferation. Mol Cell Biol. 1982 Apr;2(4):361–368. doi: 10.1128/mcb.2.4.361. [DOI] [PMC free article] [PubMed] [Google Scholar]

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