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. 1989 Sep;91(1):445–449. doi: 10.1104/pp.91.1.445

Maize Photosystem I 1

Identification of the Subunit which Binds Plastocyanin

R Max Wynn 1, Christine Luong 1, Richard Malkin 1
PMCID: PMC1062013  PMID: 16667040

Abstract

Photosystem I (PSI) has been isolated from mesophyll chloroplasts of mature maize leaves. The isolated PSI (PSI-200) was used as starting material for preparing an antenna-depleted core (PSI-100). Both of these preparations appear to be quite analogous to PSI complexes isolated from other plant tissue sources, such as those from C3 plants, as judged from NADP photoreduction assays, immunoblotting, and the ability of the complexes to form a covalent crosslinked product with spinach plastocyanin. The study suggests that the PSI complex from a C4 plant is similar to that isolated from a C3 plant in that both contain the plastocyanin docking protein although the apparent molecular weight of these respective subunits differ slightly.

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Selected References

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  1. Anderson J. M. P-700 content and polypeptide profile of chlorophyll-protein complexes of spinach and barley thylakoids. Biochim Biophys Acta. 1980 Jun 10;591(1):113–126. doi: 10.1016/0005-2728(80)90225-x. [DOI] [PubMed] [Google Scholar]
  2. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bengis C., Nelson N. Subunit structure of chloroplast photosystem I reaction center. J Biol Chem. 1977 Jul 10;252(13):4564–4569. [PubMed] [Google Scholar]
  4. Bruce B. D., Malkin R. Subunit stoichiometry of the chloroplast photosystem I complex. J Biol Chem. 1988 May 25;263(15):7302–7308. [PubMed] [Google Scholar]
  5. Dunn P. P., Packman L. C., Pappin D., Gray J. C. N-terminal amino acid sequence analysis of the subunits of pea photosystem I. FEBS Lett. 1988 Feb 8;228(1):157–161. doi: 10.1016/0014-5793(88)80607-0. [DOI] [PubMed] [Google Scholar]
  6. Golbeck J. H. Structure, function and organization of the Photosystem I reaction center complex. Biochim Biophys Acta. 1987;895(3):167–204. doi: 10.1016/s0304-4173(87)80002-2. [DOI] [PubMed] [Google Scholar]
  7. Hayashida N., Matsubayashi T., Shinozaki K., Sugiura M., Inoue K., Hiyama T. The gene for the 9 kd polypeptide, a possible apoprotein for the iron-sulfur centers A and B of the photosystem I complex, in tobacco chloroplast DNA. Curr Genet. 1987;12(4):247–250. doi: 10.1007/BF00435285. [DOI] [PubMed] [Google Scholar]
  8. Hiyama T., Ke B. Difference spectra and extinction coefficients of P 700 . Biochim Biophys Acta. 1972 Apr 20;267(1):160–171. doi: 10.1016/0005-2728(72)90147-8. [DOI] [PubMed] [Google Scholar]
  9. Høj P. B., Møller B. L. The 110-kDa reaction center protein of photosystem I, P700-chlorophyll a-protein 1, is an iron-sulfur protein. J Biol Chem. 1986 Oct 25;261(30):14292–14300. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Lagoutte B. Cloning and sequencing of spinach cDNA clones encoding the 20 kDa PS I polypeptide. FEBS Lett. 1988 May 23;232(2):275–280. doi: 10.1016/0014-5793(88)80752-x. [DOI] [PubMed] [Google Scholar]
  12. Lam E., Oritz W., Mayfield S., Malkin R. Isolation and Characterization of a Light-Harvesting Chlorophyll a/b Protein Complex Associated with Photosystem I. Plant Physiol. 1984 Mar;74(3):650–655. doi: 10.1104/pp.74.3.650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McSwain B. D., Arnon D. I. Enhancement effects and the identity of the two photochemical reactions of photosynthesis. Proc Natl Acad Sci U S A. 1968 Nov;61(3):989–996. doi: 10.1073/pnas.61.3.989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mullet J. E., Burke J. J., Arntzen C. J. Chlorophyll proteins of photosystem I. Plant Physiol. 1980 May;65(5):814–822. doi: 10.1104/pp.65.5.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nechushtai R., Nelson N. Photosystem I reaction centers from Chlamydomonas and higher plant chloroplasts. J Bioenerg Biomembr. 1981 Dec;13(5-6):295–306. doi: 10.1007/BF00743207. [DOI] [PubMed] [Google Scholar]
  16. Nechushtai R., Schuster G., Nelson N., Ohad I. Photosystem I reaction centers from maize bundle-sheath and mesophyll chloroplasts lack subunit III. Eur J Biochem. 1986 Aug 15;159(1):157–161. doi: 10.1111/j.1432-1033.1986.tb09846.x. [DOI] [PubMed] [Google Scholar]
  17. Oh-oka H., Takahashi Y., Kuriyama K., Saeki K., Matsubara H. The protein responsible for center A/B in spinach photosystem I: isolation with iron-sulfur cluster(s) and complete sequence analysis. J Biochem. 1988 Jun;103(6):962–968. doi: 10.1093/oxfordjournals.jbchem.a122394. [DOI] [PubMed] [Google Scholar]
  18. Steppuhn J., Hermans J., Nechushtai R., Ljungberg U., Thümmler F., Lottspeich F., Herrmann R. G. Nucleotide sequence of cDNA clones encoding the entire precursor polypeptides for subunits IV and V of the photosystem I reaction center from spinach. FEBS Lett. 1988 Sep 12;237(1-2):218–224. doi: 10.1016/0014-5793(88)80205-9. [DOI] [PubMed] [Google Scholar]
  19. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vierling E., Alberte R. S. P(700) Chlorophyll a-Protein : Purification, Characterization, and Antibody Preparation. Plant Physiol. 1983 Jul;72(3):625–633. doi: 10.1104/pp.72.3.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wynn R. M., Malkin R. Interaction of plastocyanin with photosystem I: a chemical cross-linking study of the polypeptide that binds plastocyanin. Biochemistry. 1988 Aug 9;27(16):5863–5869. doi: 10.1021/bi00416a007. [DOI] [PubMed] [Google Scholar]
  22. Zanetti G., Merati G. Interaction between photosystem I and ferredoxin. Identification by chemical cross-linking of the polypeptide which binds ferredoxin. Eur J Biochem. 1987 Nov 16;169(1):143–146. doi: 10.1111/j.1432-1033.1987.tb13591.x. [DOI] [PubMed] [Google Scholar]
  23. Zilber A. L., Malkin R. Ferredoxin Cross-Links to a 22 kD Subunit of Photosystem I. Plant Physiol. 1988 Nov;88(3):810–814. doi: 10.1104/pp.88.3.810. [DOI] [PMC free article] [PubMed] [Google Scholar]

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