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. 1992 May;99(1):324–328. doi: 10.1104/pp.99.1.324

Enzymic Components of Sucrose Accumulation in the Wild Tomato Species Lycopersicon peruvianum

John R Stommel 1,2
PMCID: PMC1080443  PMID: 16668869

Abstract

Sugar and soluble solids content and invertase (EC 3.2.1.26), sucrose synthase (EC 2.4.1.13), and sucrose phosphate synthase (EC 2.4.1.14) enzyme activities were measured throughout fruit development in tomato (Lycopersicon esculentum Mill.) and the green fruited species Lycopersicon peruvianum. Fruit of L. peruvianum accumulated predominantly sucrose, in contrast with hexose accumulation, which is characteristic of L. esculentum. The percentage of soluble solids in ripe L. peruvianum fruit was more than twice that present in L. esculentum and attributed primarily to the high level of sucrose accumulated in L. peruvianum. Low levels of invertase and sucrose synthase activity were associated with the period of significant sucrose accumulation and storage in L. peruvianum. Increased sucrose phosphate synthase activity was observed during the latter stages of fruit development in sucrose-accumulating fruit but was not coincident with maximum rates of sucrose accumulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Damon S., Hewitt J., Nieder M., Bennett A. B. Sink Metabolism in Tomato Fruit : II. Phloem Unloading and Sugar Uptake. Plant Physiol. 1988 Jul;87(3):731–736. doi: 10.1104/pp.87.3.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Giaquinta R. T. Sucrose translocation and storage in the sugar beet. Plant Physiol. 1979 May;63(5):828–832. doi: 10.1104/pp.63.5.828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hatch M. D., Glasziou K. T. Sugar Accumulation Cycle in Sugar Cane. II. Relationship of Invertase Activity to Sugar Content & Growth Rate in Storage Tissue of Plants Grown in Controlled Environments. Plant Physiol. 1963 May;38(3):344–348. doi: 10.1104/pp.38.3.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Helmerhorst E., Stokes G. B. Microcentrifuge desalting: a rapid, quantitative method for desalting small amounts of protein. Anal Biochem. 1980 May 1;104(1):130–135. doi: 10.1016/0003-2697(80)90287-0. [DOI] [PubMed] [Google Scholar]
  5. Hubbard N. L., Huber S. C., Pharr D. M. Sucrose Phosphate Synthase and Acid Invertase as Determinants of Sucrose Concentration in Developing Muskmelon (Cucumis melo L.) Fruits. Plant Physiol. 1989 Dec;91(4):1527–1534. doi: 10.1104/pp.91.4.1527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Miron D., Schaffer A. A. Sucrose Phosphate Synthase, Sucrose Synthase, and Invertase Activities in Developing Fruit of Lycopersicon esculentum Mill. and the Sucrose Accumulating Lycopersicon hirsutum Humb. and Bonpl. Plant Physiol. 1991 Feb;95(2):623–627. doi: 10.1104/pp.95.2.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Van Handel E. Direct microdetermination of sucrose. Anal Biochem. 1968 Feb;22(2):280–283. doi: 10.1016/0003-2697(68)90317-5. [DOI] [PubMed] [Google Scholar]
  8. Yelle S., Chetelat R. T., Dorais M., Deverna J. W., Bennett A. B. Sink Metabolism in Tomato Fruit : IV. Genetic and Biochemical Analysis of Sucrose Accumulation. Plant Physiol. 1991 Apr;95(4):1026–1035. doi: 10.1104/pp.95.4.1026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Yelle S., Hewitt J. D., Robinson N. L., Damon S., Bennett A. B. Sink Metabolism in Tomato Fruit : III. Analysis of Carbohydrate Assimilation in a Wild Species. Plant Physiol. 1988 Jul;87(3):737–740. doi: 10.1104/pp.87.3.737. [DOI] [PMC free article] [PubMed] [Google Scholar]

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