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. 1976 May;73(5):1717–1721. doi: 10.1073/pnas.73.5.1717

Effects of concanavalin A and a succinylated derivative on lymphocyte proliferation and cyclic nucleotide levels.

J W Hadden, E M Hadden, J R Sadlik, R G Coffey
PMCID: PMC430371  PMID: 179096

Abstract

To better define cell surface-related changes involved in lymphocyte activation, we studied native concanavalin A (Con A) and succinylated concanavalin A (Suc-Con A) for their effects on proliferation and cyclic nucleotide levels of human peripheral blood lymphocytes. At optimal mitogenic concentrations, the two forms of Con A induce equivalent proliferation; however, the mitogenic activity of Con A progressively decreases above 50 mug/ml. In contrast, the mitogenic activity of Suc-Con A is not decreased even at 250 mug/ml.

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Selected References

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  1. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  2. DeRubertis F. R., Zenser T. V., Adler W. H., Hudson T. Role of cyclic adenosine 3',5'-monophosphate in lymphocyte mitogenesis. J Immunol. 1974 Jul;113(1):151–161. [PubMed] [Google Scholar]
  3. Durham J. P., Baserga R., Butcher F. R. The effect of isoproterenol and its analogs upon adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate levels in mouse parotid gland in vivo. Relationship to the stimulation of DNA synthesis. Biochim Biophys Acta. 1974 Nov 4;372(1):196–217. doi: 10.1016/0304-4165(74)90087-7. [DOI] [PubMed] [Google Scholar]
  4. Folch H., Yoshinaga M., Waksman B. H. Regulation of lymphocyte responses in vitro. 3. Inhibition by adherent cells of the T-lymphocyte response to phytohemagglutinin. J Immunol. 1973 Mar;110(3):835–839. [PubMed] [Google Scholar]
  5. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greaves M. F., Bauminger S. Activation of T and B lymphocytes by insoluble phytomitogens. Nat New Biol. 1972 Jan 19;235(55):67–70. doi: 10.1038/newbio235067a0. [DOI] [PubMed] [Google Scholar]
  7. Greaves M., Janossy G., Doenhoff M. Selective triggering of human T and B lymphocytes in vitro by polyclonal mitogens. J Exp Med. 1974 Jul 1;140(1):1–18. doi: 10.1084/jem.140.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gunther G. R., Wang J. L., Yahara I., Cunningham B. A., Edelman G. M. Concanavalin A derivatives with altered biological activities. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1012–1016. doi: 10.1073/pnas.70.4.1012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hadden J. W., Hadden E. M., Middleton E., Jr Lymphocyte blast transformation. I. Demonstration of adrenergic receptors in human peripheral lymphocytes. Cell Immunol. 1970 Dec;1(6):583–595. doi: 10.1016/0008-8749(70)90024-9. [DOI] [PubMed] [Google Scholar]
  10. Hartzman R. J., Bach M. L., Bach F. H., Thurman G. B., Sell K. W. Precipitation of radioactively labeled samples: a semi-automatic multiple-sample processor. Cell Immunol. 1972 Jun;4(2):182–186. doi: 10.1016/0008-8749(72)90018-4. [DOI] [PubMed] [Google Scholar]
  11. Hirschhorn R., Grossman J., Weissmann G. Effect of cyclic 3',5'-adenosine monophosphate and theophylline on lymphocyte transformation. Proc Soc Exp Biol Med. 1970 Apr;133(4):1361–1365. doi: 10.3181/00379727-133-34690. [DOI] [PubMed] [Google Scholar]
  12. Johnson E. M., Hadden J. W. Phosphorylation of lymphocyte nuclear acidic proteins: regulation by cyclic nucleotides. Science. 1975 Mar 28;187(4182):1198–1200. doi: 10.1126/science.163491. [DOI] [PubMed] [Google Scholar]
  13. Johnson E. M., Inoue A., Crouse L. J., Allfrey V. G., Hadden J. W. Effects of cyclic GMP upon DNA binding by a calf thymus nuclear proteins fraction. Biochem Biophys Res Commun. 1975 Jul 22;65(2):714–721. doi: 10.1016/s0006-291x(75)80204-x. [DOI] [PubMed] [Google Scholar]
  14. Johnson L. D., Hadden J. W. Cyclic GMP and lymphocyte proliferation: effects on DNA-dependent RNA polymerase I and II activities. Biochem Biophys Res Commun. 1975 Oct 27;66(4):1498–1505. doi: 10.1016/0006-291x(75)90528-8. [DOI] [PubMed] [Google Scholar]
  15. Kuehl F. A., Jr, Ham E. A., Zanetti M. E., Sanford C. H., Nicol S. E., Goldberg N. D. Estrogen-related increases in uterine guanosine 3':5'-cyclic momophosphate levels. Proc Natl Acad Sci U S A. 1974 May;71(5):1866–1870. doi: 10.1073/pnas.71.5.1866. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Lohrmann H. P., Novikovs L., Graw R. G., Jr Cellular interactions in the proliferative response of human T and B lymphocytes to phytomitogens and allogeneic lymphocytes. J Exp Med. 1974 Jun 1;139(6):1553–1567. doi: 10.1084/jem.139.6.1553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lyle L. R., Parker C. W. Cylic adenosine 3',5'-monophosphate responses to concanavalin A in human lymphocytes. Evidence that the response involves specific carbohydrate receptors on the cell surface. Biochemistry. 1974 Dec 17;13(26):5415–5420. doi: 10.1021/bi00723a027. [DOI] [PubMed] [Google Scholar]
  19. Makman M. H. Properties of adenylate cyclase of lymphoid cells. Proc Natl Acad Sci U S A. 1971 May;68(5):885–889. doi: 10.1073/pnas.68.5.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Murad F., Manganiello V., Vaughan M. A simple, sensitive protein-binding assay for guanosine 3':5'-monophosphate. Proc Natl Acad Sci U S A. 1971 Apr;68(4):736–739. doi: 10.1073/pnas.68.4.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Novogrodsky A., Katchalski E. Effect of phytohemagglutinin and prostaglandins on cyclic AMP synthesis in rat lymph node lymphocytes. Biochim Biophys Acta. 1970 Aug 14;215(2):291–296. doi: 10.1016/0304-4165(70)90027-9. [DOI] [PubMed] [Google Scholar]
  22. Novogrodsky A., Katchalski E. Lymphocyte transformation induced by concanavalin A and its reversion by methyl-alpha-D-mannopyranoside. Biochim Biophys Acta. 1971 Jan 28;228(2):579–583. doi: 10.1016/0005-2787(71)90064-5. [DOI] [PubMed] [Google Scholar]
  23. Ong S. H., Whitley T. H., Stowe N. W., Steiner A. L. Immunohistochemical localization of 3': 5'-cyclic AMP and 3': 5'-cyclic GMP in rat liver, intestine, and testis. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2022–2026. doi: 10.1073/pnas.72.6.2022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sawyer W. H., Hammarström S., Möller G., Goldstein I. J. Precipitin and mitogenic behavior of dimeric and tetrameric concanavalin A. Eur J Immunol. 1976 Jul;5(7):507–510. doi: 10.1002/eji.1830050717. [DOI] [PubMed] [Google Scholar]
  25. Schreiner G. F., Unanue E. R. The modulation of spontaneous and anti-Ig-stimulated motility of lymphocytes by cyclic nucleotides and adrenergic and cholinergic agents. J Immunol. 1975 Feb;114(2 Pt 2):802–808. [PubMed] [Google Scholar]
  26. Schultz G., Hardman J. G., Schultz K., Davis J. W., Sutherland E. W. A new enzymatic assay for guanosine 3':5'-cyclic monophosphate and its application to the ductus deferens of the rat. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1721–1725. doi: 10.1073/pnas.70.6.1721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Schumm D. E., Morris H. P., Webb T. E. Early biochemical changes in phytohemagglutinin-stimulated peripheral blood lymphocytes from normal and tumor-bearing rats. Eur J Cancer. 1974 Feb;10(2):107–113. doi: 10.1016/0014-2964(74)90061-9. [DOI] [PubMed] [Google Scholar]
  28. Seifert W. E., Rudland P. S. Possible involvement of cyclic GMP in growth control of cultured mouse cells. Nature. 1974 Mar 8;248(5444):138–140. doi: 10.1038/248138a0. [DOI] [PubMed] [Google Scholar]
  29. Sloboda R. D., Rudolph S. A., Rosenbaum J. L., Greengard P. Cyclic AMP-dependent endogenous phosphorylation of a microtubule-associated protein. Proc Natl Acad Sci U S A. 1975 Jan;72(1):177–181. doi: 10.1073/pnas.72.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Smith J. W., Steiner A. L., Parker C. W. Human lymphocytic metabolism. Effects of cyclic and noncyclic nucleotides on stimulation by phytohemagglutinin. J Clin Invest. 1971 Feb;50(2):442–448. doi: 10.1172/JCI106511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Steiner A. L., Parker C. W., Kipnis D. M. The measurement of cyclic nucleotides by radioimmunoassay. Adv Biochem Psychopharmacol. 1970;3:89–111. [PubMed] [Google Scholar]
  32. Sörén L. Variability of the time at which PHA-stimulated lymphocytes initiate DNA synthesis. Exp Cell Res. 1973 Mar 30;78(1):201–208. doi: 10.1016/0014-4827(73)90055-4. [DOI] [PubMed] [Google Scholar]
  33. Wang J. L., McClain D. A., Edelman G. M. Modulation of lymphocyte mitogenesis. Proc Natl Acad Sci U S A. 1975 May;72(5):1917–1921. doi: 10.1073/pnas.72.5.1917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Watson J. The influence of intracellular levels of cyclic nucleotides on cell proliferation and the induction of antibody synthesis. J Exp Med. 1975 Jan 1;141(1):97–111. doi: 10.1084/jem.141.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Webb D. R., Belohradsky B., Hanes D., Stites D. P., Perlman J. D., Fudenberg H. H. Control of mitogen-induced lymphocyte activation. 11. Analysis of cell populations and metabolic events involved in cyclic AMP-mediated recovery of DNA synthesis suppressed by mitogens. Clin Immunol Immunopathol. 1975 Jul;4(2):226–240. doi: 10.1016/0090-1229(75)90058-6. [DOI] [PubMed] [Google Scholar]
  36. Wedner H. J., Dankner R., Parker C. W. Cyclic GMP and lectin-induced lymphocyte activation. J Immunol. 1975 Dec;115(6):1682–1687. [PubMed] [Google Scholar]
  37. Wedner H. J., Parker C. W. Protein phosphorylation in human peripheral lymphocytes - stimulation by phytohemagglutinin and N6 monobutyryl cyclic AMP. Biochem Biophys Res Commun. 1975 Feb 17;62(4):808–815. doi: 10.1016/0006-291x(75)90394-0. [DOI] [PubMed] [Google Scholar]
  38. Whitfield J. F., MacManus J. P., Boynton A. L., Gillan D. J., Isaacs R. J. Concanavalin A and the initiation of thymic lymphoblast DNA synthesis and proliferation by a calcium-dependent increase in cyclic GMP level. J Cell Physiol. 1974 Dec;84(3):445–458. doi: 10.1002/jcp.1040840312. [DOI] [PubMed] [Google Scholar]
  39. Whitfield J. F., Rixon R. H., MacManus J. P., Balk S. D. Calcium, cyclic adenosine 3',5'-monophosphate, and the control of cell proliferation: a review. In Vitro. 1973 Jan-Feb;8(4):257–278. doi: 10.1007/BF02615905. [DOI] [PubMed] [Google Scholar]

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