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. 1978 Jan;19(1):152–159. doi: 10.1128/iai.19.1.152-159.1978

Extraction of skin test activity from Coccidioides immitis mycelia by water, perchloric acid, and aqueous phenol extraction.

R W Wheat, K S Su Chung, E P Ornellas, E R Scheer
PMCID: PMC414061  PMID: 203531

Abstract

Water, perchloric acid extracts, and fractions of partially defatted whole mycelia of Coccidioides immitis contained delayed skin test activity when tested in C. immitis-infected guinea pits. Aqueous phenol extraction of these fractions resulted in partitioning of activity between aqueous-soluble and phenol-soluble fractions; activity was found to be water soluble after removal of phenol by extensive dialysis. Highest specific activity skin test antigen was invariably found in the phenol-soluble phase, water-soluble fraction. Material of equivalent activity could also be extracted directly from the defatted mycelia. Skin test active fractions contained glucose, mannose, 3-O-methylmannose, glucosamine, and amino acids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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