Abstract
A rapid method has been developed for measuring the binding capacity of asbestos and other mineral fibres for environmental carcinogens. Benzo(alpha)pyrene (B(alpha)P), nitrosonornicotine (NNN), and N-acetyl-2-aminofluorene (NAAF) were assayed in the presence of Canadian grade 4T30 chrysotile, chrysotile A, amosite, crocidolite, glass microfibres, glasswool, attapulgite, and titanium dioxide. Chrysotile binds significantly more carcinogens than the other mineral fibres. This binding assay is reproducible with coefficients of variation of less than 8% and 6% respectively for inter and intra assay. The influence of pH was also studied, and there is good correlation between the carcinogen binding and the charge of the tested mineral fibres. The in vitro cytotoxicity on macrophage like cell line P388D1 and the haemolytic activity of various mineral fibres were also measured; a good correlation was found between the binding capacity and the cytotoxicity of tested mineral fibres on P388D1 cells. These results give some explanations for the reported synergism between exposure to asbestos and the smoking habits of workers.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Eastman A., Mossman B. T., Bresnick E. Formation and removal of benzo(a)pyrene adducts of DNA in hamster tracheal epithelial cells. Cancer Res. 1981 Jul;41(7):2605–2610. [PubMed] [Google Scholar]
- Kandaswami C., O'Brien P. J. Effects of asbestos on membrane transport and metabolism of benzo(a)pyrene. Biochem Biophys Res Commun. 1980 Nov 28;97(2):794–801. doi: 10.1016/0006-291x(80)90334-4. [DOI] [PubMed] [Google Scholar]
- Langer A. M., Rohl A. N., Selikoff I. J., Harlow G. E., Prinz M. Asbestos as a cofactor in carcinogenesis among nickel-processing workers. Science. 1980 Jul 18;209(4454):420–422. doi: 10.1126/science.7384817. [DOI] [PubMed] [Google Scholar]
- McMillan G. H., Pethybridge R. J., Sheers G. Effect of smoking on attack rates of pulmonary and pleural lesions related to exposure to asbestos dust. Br J Ind Med. 1980 Aug;37(3):268–272. doi: 10.1136/oem.37.3.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller L., Smith W. E., Berliner S. W. Tests for effect of asbestos on benzo[a]pyrene carcinogenesis in the respiratory tract. Ann N Y Acad Sci. 1965 Dec 31;132(1):489–500. doi: 10.1111/j.1749-6632.1965.tb41128.x. [DOI] [PubMed] [Google Scholar]
- Moore G. E., Gerner R. E., Franklin H. A. Culture of normal human leukocytes. JAMA. 1967 Feb 20;199(8):519–524. [PubMed] [Google Scholar]
- Morgan A., Holmes A. Concentrations and dimensions of coated and uncoated asbestos fibres in the human lung. Br J Ind Med. 1980 Feb;37(1):25–32. doi: 10.1136/oem.37.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roy-Chowdhury A. K., Mooney T. F., Jr, Reeves A. L. Trace metals in asbestos carcinogenesis. Arch Environ Health. 1973 May;26(5):253–255. doi: 10.1080/00039896.1973.10666269. [DOI] [PubMed] [Google Scholar]
- SELIKOFF I. J., CHURG J., HAMMOND E. C. RELATION BETWEEN EXPOSURE TO ASBESTOS AND MESOTHELIOMA. N Engl J Med. 1965 Mar 18;272:560–565. doi: 10.1056/NEJM196503182721104. [DOI] [PubMed] [Google Scholar]
- Shabad L. M., Pylev L. N., Krivosheeva L. V., Kulagina T. F., Nemenko B. A. Experimental studies on asbestos carcinogenicity. J Natl Cancer Inst. 1974 Apr;52(4):1175–1187. doi: 10.1093/jnci/52.4.1175. [DOI] [PubMed] [Google Scholar]
- Speil S., Leineweber J. P. Asbestos minerals in modern technology. Environ Res. 1969 Apr;2(3):166–208. doi: 10.1016/0013-9351(69)90036-x. [DOI] [PubMed] [Google Scholar]
- Stanton M. F., Wrench C. Mechanisms of mesothelioma induction with asbestos and fibrous glass. J Natl Cancer Inst. 1972 Mar;48(3):797–821. [PubMed] [Google Scholar]