Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1990 Oct;94(2):485–491. doi: 10.1104/pp.94.2.485

In Vitro Oxidation of Indoleacetic Acid by Soluble Auxin-Oxidases and Peroxidases from Maize Roots

Roland Beffa 1,1, Hilary V Martin 1, Paul-Emile Pilet 1
PMCID: PMC1077258  PMID: 16667738

Abstract

Soluble auxin-oxidases were extracted from Zea mays L. cv LG11 apical root segments and partially separated from peroxidases (EC 1.11.1.7) by size-exclusion chromatography. Auxin-oxidases were resolved into one main peak corresponding to a molecular mass of 32.5 kilodaltons and a minor peak at 54.5 kilodaltons. Peroxidases were separated into at least four peaks, with molecular masses from 32.5 to 78 kilodaltons. In vitro activity of indoleacetic acid-oxidases was dependent on the presence of MnCl2 and p-coumaric acid. Compound(s) present in the crude extract and several synthetic auxin transport inhibitors (including 2,3,5-triiodobenzoic acid and N-1-naphthylphthalamic acid) inhibited auxin-oxidase activity, but had no effect on peroxidases. The products resulting from the in vitro enzymatic oxidation of [3H] indoleacetic acid were separated by HPLC and the major metabolite was found to cochromatograph with indol-3yl-methanol.

Full text

PDF
485

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. HINMAN R. L., LANG J. PEROXIDASE-CATALYZED OXIDATION OF INDOLE-3-ACETIC ACID. Biochemistry. 1965 Jan;4:144–158. doi: 10.1021/bi00877a023. [DOI] [PubMed] [Google Scholar]
  3. Hoyle M. C. High resolution of peroxidase-indoleacetic Acid oxidase isoenzymes from horseradish by isoelectric focusing. Plant Physiol. 1977 Nov;60(5):787–793. doi: 10.1104/pp.60.5.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jacobs M., Rubery P. H. Naturally occurring auxin transport regulators. Science. 1988 Jul 15;241(4863):346–349. doi: 10.1126/science.241.4863.346. [DOI] [PubMed] [Google Scholar]
  5. Kokkinakis D. M., Brooks J. L. Hydrogen Peroxide-mediated Oxidation of Indole-3-acetic Acid by Tomato Peroxidase and Molecular Oxygen. Plant Physiol. 1979 Aug;64(2):220–223. doi: 10.1104/pp.64.2.220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kokkinakis D. M., Brooks J. L. Tomato peroxidase: purification, characterization, and catalytic properties. Plant Physiol. 1979 Jan;63(1):93–99. doi: 10.1104/pp.63.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Martin H. V., Pilet P. E. Saturable uptake of indol-3yl-acetic Acid by maize roots. Plant Physiol. 1986 Jul;81(3):889–895. doi: 10.1104/pp.81.3.889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mumford F. E., Smith D. H., Castle J. E. An inhibitor of indoleacetic acid oxidase from pea tips. Plant Physiol. 1961 Nov;36(6):752–756. doi: 10.1104/pp.36.6.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Reinecke D. M., Bandurski R. S. Oxidation of indole-3-acetic acid to oxindole-3-acetic acid by an enzyme preparation from Zea mays. Plant Physiol. 1988;86:868–872. doi: 10.1104/pp.86.3.868. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rubery P. H. Studies on indoleacetic acid oxidation by liquid medium from crown gall tissue culture cells: the role of malic acid and related compounds. Biochim Biophys Acta. 1972 Jan 28;261(1):21–34. doi: 10.1016/0304-4165(72)90308-x. [DOI] [PubMed] [Google Scholar]
  11. Rutschmann M., Kuehn L., Dahlmann B., Reinauer H. High resolution gel chromatography of proteins. Anal Biochem. 1982 Jul 15;124(1):134–138. doi: 10.1016/0003-2697(82)90230-5. [DOI] [PubMed] [Google Scholar]
  12. Sabater F., Sánchez-Bravo J., Acosta M. Effects of enzyme/substrate ratio and of cofactors on the oxidation products of indole-3-acetic acid catalyzed by peroxidase. Rev Esp Fisiol. 1983 Jun;39(2):169–173. [PubMed] [Google Scholar]
  13. Sequeira L., Mineo L. Partial purification and kinetics of indoleacetic Acid oxidase from tobacco roots. Plant Physiol. 1966 Sep;41(7):1200–1208. doi: 10.1104/pp.41.7.1200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sundberg B., Sandberg G., Jensen E. Identification and Quantification of Indole-3-methanol in Etiolated Seedlings of Scots Pine (Pinus sylvestris L.). Plant Physiol. 1985 Apr;77(4):952–955. doi: 10.1104/pp.77.4.952. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES