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. 1990 Dec 15;272(3):721–726. doi: 10.1042/bj2720721

Isolation and characterization of BanLec-I, a mannoside-binding lectin from Musa paradisiac (banana).

V L Koshte 1, W van Dijk 1, M E van der Stelt 1, R C Aalberse 1
PMCID: PMC1149768  PMID: 2268297

Abstract

A lectin (BanLec-I) from banana (Musa paradisiac) with a binding specificity for oligomannosidic glycans of size classes higher than (Man)6GlcNAc was isolated and purified by affinity chromatography on a Sephadex G-75 column. It did not agglutinate untreated human or sheep erythrocytes, but it did agglutinate rabbit erythrocytes. BanLec-I stimulated T-cell proliferation. On size-exclusion chromatography, BanLec-I has a molecular mass of approx. 27 kDa, and on SDS/PAGE the molecular mass is approx. 13 kDa. The isoelectric point is 7.2-7.5. BanLec-I was found to be very effective as a probe in detecting glycoproteins, e.g. on nitrocellulose blots.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aalberse R. C., Koshte V., Clemens J. G. Immunoglobulin E antibodies that crossreact with vegetable foods, pollen, and Hymenoptera venom. J Allergy Clin Immunol. 1981 Nov;68(5):356–364. doi: 10.1016/0091-6749(81)90133-0. [DOI] [PubMed] [Google Scholar]
  2. Björkstén F., Halmepuro L., Hannuksela M., Lahti A. Extraction and properties of apple allergens. Allergy. 1980 Dec;35(8):671–677. doi: 10.1111/j.1398-9995.1980.tb02020.x. [DOI] [PubMed] [Google Scholar]
  3. Chapman M. D., Aalberse R. C., Brown M. J., Platts-Mills T. A. Monoclonal antibodies to the major feline allergen Fel d I. II. Single step affinity purification of Fel d I, N-terminal sequence analysis, and development of a sensitive two-site immunoassay to assess Fel d I exposure. J Immunol. 1988 Feb 1;140(3):812–818. [PubMed] [Google Scholar]
  4. Chapman M. D., Platts-Mills T. A. Purification and characterization of the major allergen from Dermatophagoides pteronyssinus-antigen P1. J Immunol. 1980 Aug;125(2):587–592. [PubMed] [Google Scholar]
  5. Cottam G. P., Moran D. M., Standring R. Physicochemical and immunochemical characterization of allergenic proteins from rye-grass (Lolium perenne) pollen prepared by a rapid and efficient purification method. Biochem J. 1986 Mar 1;234(2):305–310. doi: 10.1042/bj2340305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Diabaté S., Geyer R., Stirm S. Structure of the major oligosaccharides in the fusion glycoprotein of Newcastle disease virus. Eur J Biochem. 1984 Mar 1;139(2):329–336. doi: 10.1111/j.1432-1033.1984.tb08011.x. [DOI] [PubMed] [Google Scholar]
  7. Ford S. A., Baldo B. A. A re-examination of ryegrass (Lolium perenne) pollen allergens. Int Arch Allergy Appl Immunol. 1986;81(3):193–203. doi: 10.1159/000234134. [DOI] [PubMed] [Google Scholar]
  8. Hoffman D. R., Shipman W. H. Allergens in bee venom. I. Separation and identification of the major allergens. J Allergy Clin Immunol. 1976 Nov;58(5):551–562. doi: 10.1016/0091-6749(76)90201-3. [DOI] [PubMed] [Google Scholar]
  9. Judd W. J., Issitt P. D., Pavone B. G., Anderson J., Aminoff D. Antibodies that define NANA-independent MN-system antigens. Transfusion. 1979 Jan-Feb;19(1):12–18. doi: 10.1046/j.1537-2995.1979.19179160260.x. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Roosnek E. E., Brouwer M. C., Aarden L. A. T cell triggering by lectins. II. Stimuli for induction of interleukin 2 responsiveness and interleukin 2 production differ only in quantitative aspects. Eur J Immunol. 1985 Jul;15(7):657–661. doi: 10.1002/eji.1830150704. [DOI] [PubMed] [Google Scholar]
  12. Shipolini R. A., Callewaert G. L., Cottrell R. C., Vernon C. A. The amino-acid sequence and carbohydrate content of phospholipase A2 from bee venom. Eur J Biochem. 1974 Oct 2;48(2):465–476. doi: 10.1111/j.1432-1033.1974.tb03787.x. [DOI] [PubMed] [Google Scholar]
  13. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

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