Erratum

Faiola B, Doyle C, Gilboa E, Nair S. Influence of CD4 T cells and the source of major histocompatibility complex class II-restricted peptides on cytotoxic T-cell priming by dendritic cells. Immunology 2002; 105:47–55.

A typesetting error resulted in Fig. 3 being omitted from this paper, and Fig. 6 printed (i.e. duplicated) in its place. The correct Fig. 3 is printed below. Also, the key to Fig. 7 was wrongly aligned. The corrected Fig. 7 is printed on the next page.

Figure 3.

Class II deficient and class II mutant DC can not prime a CTL response in vivo. Aβwt mice were immunized with 10⁵ DC which were either pulsed with 10 ng/ml OVA_257–264 class I peptide or unpulsed. Seven days post-immunization, non-adherent splenocytes were restimulated as described in materials and methods. After 5 days of restimulation, effectors were harvested and cytolytic activity was measured against peptide pulsed RMA-S target cells in a 4-hr ⁵¹Cr release assay. Similar results were obtained against EG7.OVA and EL4 targets (data not shown).

Figure 7.

In vivo CTL activity induced by AS DC presenting class II antigen is reduced in CD4-depleted mice. DC were generated in FCS or pooled AS from C57BL/6 mice. On day 8 of culture, 5 mg/ml of BSA was added to some of the AS DC cultures. Mature day 10 DC were pulsed overnight in culture with 0·05 µg/ml of either the OVA or Mut-1 H-2K^b-resticted peptide. DC were washed in PBS and 10⁵ DC were injected i.v. into non-depleted or CD4-depleted mice. Eight days post-immunization, non-adherent splenocytes were restimulated as described in Materials and Methods. BSA was added to the restimulation media of those groups where BSA was used for pulsing DC. After 5 days, CTL activity was measured against specific and non-specific targets in a 4-hr ⁵¹Cr-release assay. The mean and standard deviation of triplicate samples are plotted.

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