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. 1989 Feb;55(2):433–439. doi: 10.1128/aem.55.2.433-439.1989

Survey of the Anaerobic Biodegradation Potential of Organic Chemicals in Digesting Sludge

Nigel S Battersby 1,*, Valerie Wilson 1
PMCID: PMC184127  PMID: 16347851

Abstract

The degradation potential of 77 organic chemicals under methanogenic conditions was examined with an anaerobic digesting sludge from the United Kingdom. Degradation was assessed in terms of net total gas (CH4 plus CO2) produced, expressed as a percentage of the theoretical production (ThGP). The compounds tested were selected from various chemical groups and included substituted phenols and benzoates, pesticides, phthalic acid esters, homocyclic and heterocyclic ring compounds, glycols, and monosubstituted benzenes. The results obtained were in good agreement with published surveys of biodegradability in U.S. digesting sludges and other methanogenic environments. In general, the presence of chloro or nitro groups inhibited anaerobic gas production, while carboxyl and hydroxyl groups facilitated biodegradation. The relationship between substituent position and susceptibility to methanogenic degradation was compound dependent. The following chemicals were completely degraded (≥80% ThGP) at a concentration of 50 mg of carbon per liter: phenol, 2-aminophenol, 4-cresol, catechol, sodium benzoate, 4-aminobenzoic acid, 3-chlorobenzoic acid, phthalic acid, ethylene glycol, diethylene glycol, triethylene glycol, sodium stearate, and quinoline. 3-Cresol, 4-chlorobenzoic acid, dimethyl phthalate, and pyridine were partially degraded. Although the remaining chemicals tested were either persistent or toxic, their behavior may differ at more environmentally realistic chemical-to-biomass ratios. Our findings suggest that biodegradability assessments made with sludge from one source can be extrapolated to sludge from another source with a reasonable degree of confidence and should help in predicting the fate of an organic chemical during the anaerobic digestion of sewage sludge.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berry D. F., Francis A. J., Bollag J. M. Microbial metabolism of homocyclic and heterocyclic aromatic compounds under anaerobic conditions. Microbiol Rev. 1987 Mar;51(1):43–59. doi: 10.1128/mr.51.1.43-59.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyd S. A., Shelton D. R. Anaerobic biodegradation of chlorophenols in fresh and acclimated sludge. Appl Environ Microbiol. 1984 Feb;47(2):272–277. doi: 10.1128/aem.47.2.272-277.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyd S. A., Shelton D. R., Berry D., Tiedje J. M. Anaerobic biodegradation of phenolic compounds in digested sludge. Appl Environ Microbiol. 1983 Jul;46(1):50–54. doi: 10.1128/aem.46.1.50-54.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Grbić-Galić D., Vogel T. M. Transformation of toluene and benzene by mixed methanogenic cultures. Appl Environ Microbiol. 1987 Feb;53(2):254–260. doi: 10.1128/aem.53.2.254-260.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Healy J. B., Jr, Young L. Y. Catechol and phenol degradation by a methanogenic population of bacteria. Appl Environ Microbiol. 1978 Jan;35(1):216–218. doi: 10.1128/aem.35.1.216-218.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Healy J. B., Young L. Y. Anaerobic biodegradation of eleven aromatic compounds to methane. Appl Environ Microbiol. 1979 Jul;38(1):84–89. doi: 10.1128/aem.38.1.84-89.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hill D. W., McCarty P. L. Anaerobic degradation of selected chlorinated hydrocarbon pesticides. J Water Pollut Control Fed. 1967 Aug;39(8):1259–1277. [PubMed] [Google Scholar]
  8. Ruckdeschel G., Renner G., Schwarz K. Effects of pentachlorophenol and some of its known and possible metabolites on different species of bacteria. Appl Environ Microbiol. 1987 Nov;53(11):2689–2692. doi: 10.1128/aem.53.11.2689-2692.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Shelton D. R., Tiedje J. M. General method for determining anaerobic biodegradation potential. Appl Environ Microbiol. 1984 Apr;47(4):850–857. doi: 10.1128/aem.47.4.850-857.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Smolenski W. J., Suflita J. M. Biodegradation of cresol isomers in anoxic aquifers. Appl Environ Microbiol. 1987 Apr;53(4):710–716. doi: 10.1128/aem.53.4.710-716.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Suflita J. M., Horowitz A., Shelton D. R., Tiedje J. M. Dehalogenation: a novel pathway for the anaerobic biodegradation of haloaromatic compounds. Science. 1982 Dec 10;218(4577):1115–1117. doi: 10.1126/science.218.4577.1115. [DOI] [PubMed] [Google Scholar]
  12. Wang Y. T., Suidan M. T., Pfeffer J. T. Anaerobic biodegradation of indole to methane. Appl Environ Microbiol. 1984 Nov;48(5):1058–1060. doi: 10.1128/aem.48.5.1058-1060.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wang Y. T., Suidan M. T., Pfeffer J. T., Najm I. Effects of some alkyl phenols on methanogenic degradation of phenol. Appl Environ Microbiol. 1988 May;54(5):1277–1279. doi: 10.1128/aem.54.5.1277-1279.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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