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. 1978 Nov;5(11):4423–4430. doi: 10.1093/nar/5.11.4423

Reverse transcription of turnip yellow mosaic virus RNA primed with calf-thymus DNA hydrolysate: characterization of the purified cDNA product.

J Kummert, R Kettmann
PMCID: PMC342759  PMID: 82938

Abstract

Complementary DNA was transcribed from turnip yellow mosaic virus RNA, using the method of Taylor et al. (1). The purified cDNA thus obtained sedimented between 2 and 4 S and was a mostly uniform transcript of template RNA. It hybridized with a sharp transition to homologous TYMV-RNA (Crt 1/2 = 2.7 x 10(-2)), but showed a low level of hybridization (less than 5%) to the RNAs of two other tymoviruses, namely Andean potato latent virus and eggplant mosaic virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crossley J. R. Possible heterogeneity of 5'-terminal of turnip yellow mosaic virus RNA. Virology. 1973 Apr;52(2):578–580. doi: 10.1016/0042-6822(73)90354-1. [DOI] [PubMed] [Google Scholar]
  2. Demeure M., Kummert J., Portetelle D., Semal J. Properties of the DNA product of reverse transcription of turnip yellow mosaic virus RNA preparations. Virology. 1977 Sep;81(2):449–454. doi: 10.1016/0042-6822(77)90159-3. [DOI] [PubMed] [Google Scholar]
  3. Drohan W., Kettmann R., Colcher D., Schlom J. Isolation of the mouse mammary tumor virus sequences not transmitted as germinal provirus in the C3H and RIII mouse strains. J Virol. 1977 Mar;21(3):986–995. doi: 10.1128/jvi.21.3.986-995.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Evans R. M., Baluda M. A., Shoyab M. Differences between the integration of avian myeloblastosis virus DNA in leukemic cells and of endogenous viral DNA in normal chicken cells. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3152–3156. doi: 10.1073/pnas.71.8.3152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gould A. R., Palukaitis P., Symons R. H., Mossop D. W. Characterization of a satellite RNA associated with cucumber mosaic virus. Virology. 1978 Feb;84(2):443–455. doi: 10.1016/0042-6822(78)90261-1. [DOI] [PubMed] [Google Scholar]
  6. Gould A. R., Symons R. H. Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA. Nucleic Acids Res. 1977 Nov;4(11):3787–3802. doi: 10.1093/nar/4.11.3787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kummert J. In vitro pulse-labeling of the replicative forms of bromegrass mosaic virus RNA. Virology. 1974 Feb;57(2):314–320. doi: 10.1016/0042-6822(74)90171-8. [DOI] [PubMed] [Google Scholar]
  8. MATTHEWS R. E. Properties of nucleoprotein fractions isolated from turnip yellow mosaic virus preparations. Virology. 1960 Dec;12:521–539. doi: 10.1016/0042-6822(60)90176-8. [DOI] [PubMed] [Google Scholar]
  9. Taylor J. M., Illmensee R., Summers J. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase. Biochim Biophys Acta. 1976 Sep 6;442(3):324–330. doi: 10.1016/0005-2787(76)90307-5. [DOI] [PubMed] [Google Scholar]
  10. Vandewalle M. J., Siegel A. A study of nucleotide sequence homology between strains of tobacco mosaic virus. Virology. 1976 Sep;73(2):413–418. doi: 10.1016/0042-6822(76)90402-5. [DOI] [PubMed] [Google Scholar]
  11. Vogt V. M. Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae. Eur J Biochem. 1973 Feb 15;33(1):192–200. doi: 10.1111/j.1432-1033.1973.tb02669.x. [DOI] [PubMed] [Google Scholar]
  12. Zaitlin M., Beachy R. N., Bruening G. Lack of molecular hybridization between RNAs of two strains of TMV: a reconsideration of the criteria for strain relationships. Virology. 1977 Oct 1;82(1):237–241. doi: 10.1016/0042-6822(77)90047-2. [DOI] [PubMed] [Google Scholar]

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