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The original publication of this article included errors in Figures 2, 9, and 10, as described below. The original results and conclusions are unaffected by these corrections. The authors apologize and accept responsibility for not detecting these errors prior to publication.
Figure 2: Figure 2A shows the means ± se from three aliquots of a single RNA sample obtained from leaf tissue, pooled from four individual plants, not independent lines as stated in the published version. In Figure 2C, the DXS image appears to have been flipped and bands in the PRK immunoblot have been duplicated. These issues were not detected prior to publication. The original blots could not be found, so to resolve this, the experiment was repeated. A new set of plants was grown and immunoblot analysis performed with samples as stated in the legend. A new version of Figure 2 containing these data is shown below.
Figure 2.
Original: Molecular and Biochemical Characterization of the TKox Plants.
(A) Quantitative RT-PCR of the introduced plastid TK transgene.
(B) Total extractable TK activity in TKox and wild-type tobacco plants. The values in (A) and (B) represent the mean of four individual plants (se ± 4) (*P > 0.05; **P < 0.01).
(C) Immunoblot analysis of protein extracts from the newest fully expanded leaf of TKox and wild-type plants. M, mesophyll; V, vein tissue extracts. Proteins (5 μg) were separated on a 12% polyacrylamide gel and blotted onto nylon membrane and probed with polyclonal antibodies against TK, DXS, and PRK proteins.
Figure 2.
Corrected: Molecular and biochemical characterization of the TKox plants.
(A) Quantitative RT-PCR of the introduced plastid TK transgene. Values represent the mean of TK transcripts ± se of three technical replicates from a pooled single sample of RNA from four independent plants.
(B) Total extractable TK activity in TKox and wild-type tobacco plants. The values represents the mean activity level ± se of four individual plants (**P < 0.01).
(C) Immunoblot analysis of protein extracts from the newest fully expanded leaf of TKox and wild-type plants. M, mesophyll; V, vein tissue extracts. Proteins (5 μg) were separated on a 12% polyacrylamide gel, blotted onto nylon membrane, and probed initially with the polyclonal antibodies against TK and PRK proteins. The membrane was washed in stripping buffer for 30 min, blocked in 6% milk, and then probed with the DXS antibody.
Figure 9: Errors were made in the calculations to produce Figure 9, and the y axis is labeled as thiamine in μg g−1 fresh weight. The calculations were redone and the axis labeled correctly as thiamine in nmol g−1 fresh weight. The original and corrected figures are shown. The legend is unchanged from the original.
Figure 9.
Thiamine Levels Determined in TKox and Wild-Type Seeds.
Seeds were analyzed for thiamine content when dry or following 24 h in thiamine (50 mg L−1). The parental +/+ plants were watered with thiamine (50 mg L−1) throughout the life cycle. Statistical comparisons to wild-type values were made using a one-way ANOVA (95% sig) and Tukey post hoc test. Asterisk indicates significance at P < 0.05. Results are the mean ± se (n = 4).
Figure 10: The y axis in panels A and B is labeled as nmol g−1 fresh weight (TTP in A and thiamine in B), whereas for both graphs, the values are pmol g−1 fresh weight. Otherwise, both the calculations and appearance of the graph are identical to the original.
Editor's note: the corrected figures and accompanying text were reviewed by members of The Plant Cell editorial board.