CORRECTION

Chi, W., He, B., Manavski, N., Mao, J., Ji, D., Lu, C., Rochaix, J.D., Meurer, J., and Zhang, L. (2014). RHON1 mediates a Rho-like activity for transcription termination in plastids of Arabidopsis thaliana. Plant Cell 26: 4918–4932.

The authors of the above article request the publication of corrections to Figures 1 to 3, as described below, for clarification of the methods used and the manner in which gel images were arranged. The original results and conclusions are unaffected by these corrections. The authors apologize and accept responsibility for not detecting these errors prior to publication. Both original and corrected figures are shown for ease of comparison.

Figure 1.

Original: Phenotype and Gene Cloning of rhon1-2.

(D) RT-PCR (left) and immunoblot analysis (right) of RHON1 expression in wild-type, rhon1-2 mutant, and complemented plants.

Figure 2.

Original: Plastid Gene Expression of the rhon1-2 Mutants.

(A) RNA gel blot analysis of rbcL polycistronic transcript. A diagram of the rbcL polycistronic operon and the locations of the probes (a to f) are shown at the top. Blots with reduced exposure time to x-ray film for probes a and b are also included (indicated as a’ and b’). The mature forms of RNA are indicated with asterisks to the left of each blot.

(B) Run-on transcription assay of plastid genes in rhon1-2 and wild-type plants. DNA probe (200 ng) was used for hybridization with labeled transcripts. Another run-on experiment with 100 ng DNA probe was also performed and similar results were observed (Supplemental Figure 2).

Figure 2.

Corrected: Plastid Gene Expression of the rhon1-2 Mutants.

(A) RNA gel blot analysis of rbcL polycistronic transcript. A diagram of the rbcL polycistronic operon and the locations of the probes (a to f) are shown at the top. Blots with reduced exposure time to x-ray film for probes a and b are also included (indicated as a’ and b’). The mature forms of RNA are indicated with asterisks at the left of each blot. The blot of probe a was stripped and reprobed with probe f, while that of probe b was stripped and reprobed with probe d. Ethidium bromide (EtBr) staining of the RNA served as loading control.

(B) Run-on transcription assay of plastid genes in rhon1-2 and wild-type plants. A DNA probe (200 ng) was used for hybridization with labeled transcripts. Another run-on experiment with a 100 ng DNA probe was also performed, and similar results were observed (Supplemental Figure 2). The petA signal of the wild-type sample, separated by a black bar, was obtained from a different slot-blot, but all of the blots were developed and processed in parallel.

Figure 3.

Original: The Transcription Initiation Sites of Plastid Genes in Wild-Type and rhon1-2 Plants.

(A) Identification of the transcription initiation site of rbcL by 5′-RACE. Total RNA from green (without spectinomycin treatment) and white (with spectinomycin treatment) leaf tissue was ligated to an RNA linker after TAP treatment and, along with untreated RNA as a control, was subjected to RT-PCR with specific primers. The transcription initiation sites identified by sequencing of cloned RT-PCR products are underlined and marked by arrows in the diagram of the 5′ region of rbcL. The expression of the smallest PCR product in TAP-treated leaves was lower than that in leaves without TAP treatment. Therefore, this product most likely corresponds to a processing product rather than to a primary transcript (Hanaoka et al., 2003; also refer to the −70 band of [C]).

(B) Identification of the transcription initiation sites of accD. The procedure was similar to that used for rbcL.

(C) Primer extension analysis of rbcL and accD from wild-type and rhon1-2 plants. The positions of bands corresponding to transcripts with distinct 5′-ends are indicated at the right.

Figure 3.

Corrected: The Transcription Initiation Sites of Plastid Genes in Wild-Type and rhon1-2 Plants.

Figure 1D: In the corrected figure, two white vertical bars have been inserted between the rhon1-2 and rhon1-2 complemented lanes for the RHON1 and ACTIN panels, respectively, to indicate the splicing of separate lanes from the same gel in the figure. The legend of the figure has been changed accordingly for clarity.

Figure 2: In Figure 2A, the RNA gel blots with probes a and f used the same gel and nylon membrane (the first blot was stripped and reprobed with another probe); therefore, their ethidium bromide staining bands used as the loading controls were duplicated. This was also applied to the RNA gel blots of probes b and d. This duplication of loading controls is explained in the corrected legend. Images of Figure 2A have not been changed. Figure 2B has been corrected to indicate the splicing of the cemA and petA lanes of the wild-type panel by a black vertical bar. The legend of Figure 2B has been changed accordingly for clarity.

Figure 1.

Corrected: Phenotype and Gene Cloning of rhon1-2.

(D) RT-PCR (left) and immunoblot analysis (right) of RHON1 expression in wild-type, rhon1-2 mutant, and complemented plants. In the RT-PCR analysis, samples were run side-by-side on the same gel. White bars indicate noncontiguous lanes in the gel.

Figure 3B: The (+) spec and (−) spec samples were run on two separate gels and combined in the published figure. This combination was not necessary and affected the accuracy of data presentation due to the lack of a marker for this combination. Because the original data could not be found, Figure 3B was reconstituted with the data from duplicate experiments. Two separate gels from the duplicated experiments are displayed independently in the new version of Figure 3B shown below. The figure legend has not been changed.