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Figure 1D, showing the results of RNA pull-down experiments with the GST-MtRBP1 protein purified from E. coli and different regions of the Mtenod40 RNA, included an inappropriately duplicated band (the band corresponding to the full-length enod40 was a duplication of the ∆RNA band). However, the interaction between MtRBP1 and the full-length enod40 was confirmed several times and was presented also in Figure 1B. In the corrected figure, below, panel D has been replaced with the results from a separate experiment, wherein all of the indicated samples were run on a single gel as shown. The correction does not affect the results or conclusions of the manuscript, and the figure legend is unaltered from the original. The authors apologize for any inconvenience this may have caused to the readers.
Figure 1.
Interaction between MtRBP1 and enod40 RNA.
(A) The MtRBP1 cDNA was transformed into yeast strains containing RNA-expressing plasmids carrying MS2 repeats only (MS2), an MS2-enod40 fusion (enod40), and an MS2-stem construct (stem *, corresponding to the enod40 region indicated with an asterisk in [C]). Four independent transformants of each strain were plated on selective medium without His.
(B) RNA pull down analysis of stem (*) and enod40 (E40) RNAs. The input lane contains 20% of translation products used for each binding assay. The lower band may correspond to a subproduct of the in vitro translation of MtRBP1.
(C) Schematic representation of the different regions spanning conserved stem-loops along the enod40 RNA (lines; Girard et al., 2003). The stem-loop indicated with an asterisk corresponds to the one previously predicted by Sousa et al. (2001). Boxes I and II are indicated as rectangular boxes.
(D) RNA pull down experiments with the GST-MtRBP1 protein purified from E. coli and different regions of the Mtenod40 RNA schematized in (C). C- is a control reaction in which no RNA was included.
Footnotes
Editor’s note: the corrected figure and accompanying text were reviewed by members of The Plant Cell editorial board.
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