Peng, L., Ma, J., Chi, W., Guo, J., Zhu, S., Lu, Q., Lu, C., and Zhang, L. (2006). LOW PSII ACCUMULATION1 is involved in efficient assembly of photosystem II in Arabidopsis thaliana. Plant Cell 18: 955–969.
The authors of the above article request the publication of corrected Figures 1, 6, 8, 10, and 11, as described below. The original results and conclusions are unaffected by these corrections. The authors apologize for the inconvenience caused. All authors approve this correction.
Figure 1.
Corrected: Phenotypes of lpa1 Mutant and Wild-Type Plants.
(A) Four-week-old plants grown in the growth chamber.
Figure 6.
In Vivo Protein Synthesis of Plastid-Encoded Membrane Proteins and Immunoblot Analysis after Second Dimension BN-PAGE.
(A) Incorporation of [35S]Met into thylakoid membrane proteins in young seedlings. After a 20-min pulse in Arabidopsis young seedlings in the presence of cycloheximide, the thylakoid membranes were isolated, and the proteins were separated by SDS-urea-PAGE and visualized autoradiographically.
(B) Pulse and chase of thylakoid membrane proteins. The 20-min pulse in Arabidopsis young seedlings was followed by a chase of cold Met for 1, 2, and 4 h. The thylakoid membranes were then isolated, separated by SDS-urea-PAGE, and visualized autoradiographically. A black bar indicates noncontiguous lanes from the same gel.
(C) Incorporation of [35S]Met into thylakoid membrane proteins in mature leaves. After a 30-min pulse in Arabidopsis mature leaves in the presence of cycloheximide, the thylakoid membranes were isolated, and the proteins were separated by SDS-urea-PAGE and visualized by autoradiogram.
Figure 8.
Corrected: RT-PCR Analysis of Mutant Plants.
RT-PCR was performed with specific primers for At1g02900, At1g02910, and At1g02920 and actin-specific primers.
Figure 10.
Immunolocalization of LPA1 and Light-Induced Accumulation of LPA1 and Plastid Proteins.
(A) Immunoblot analysis of LPA1. Samples from wild-type and lpa1 plants consisting of total leaf proteins, the thylakoids (equivalent to 5 mg chlorophyll), RNCs, and RNCs treated with 1 mM puromycin or 10 mM EDTA (equivalent to 50 mg chlorophyll) were separated by SDS-PAGE and immunodetected with the antibody raised against LPA1. A black bar separates independent immunoblots obtained from two independent experiments.
(B) Salt washing of the membranes. The membrane preparations were incubated with 1 M NaCl, 200 mM Na2CO3, 1 M CaCl2, and 6 M urea for 30 min at 0°C (top panel) or sonicated in the presence of these salts (bottom panel) and incubated for another 30 min at 0°C. PsbO, the 33-kD luminal protein of PSII, was used as a marker. CK, the membrane preparations without treatments were used as a control.
(C) Immunoblot analysis of protein accumulation during light-induced greening of etiolated Arabidopsis seedlings. After growth in the dark for 5 d, the etiolated seedlings were illuminated for 4, 8, 24, and 48 h. The samples were harvested and processed for immunoblot analysis. Protein loadings were based on equal amounts of seedling fresh weight. The antibodies used for the analysis are indicated at the right.
Figure 11.
Sucrose Density Gradient Analysis of LPA1 in Thylakoids.
Thylakoids (0.5 mg/mL) were solubilized with 1% DM and separated in a linear 0.1 to 1 M sucrose gradient. Twenty fractions were collected from the gradients, and the proteins from each fraction were separated by SDS-PAGE. After electrophoresis, the separated proteins were immunodetected with the antibody directed against LPA1. To identify the positions of the major photosynthetic complexes, blots were also probed with anti-D2, anti-CP47, anti-LHCII, and anti-PsaA/B antibodies. The positions of 669-kD (thyroglobulin), 440-kD (ferritin), 232-kD (catalase), 140-kD (lactate dehydrogenase), and 66-kD (BSA) molecular size markers are indicated. Black bars separate independent immunoblots obtained from two different gels in the same experiment.
Figure 1.
Original: Phenotypes of lpa1 Mutant and Wild-Type Plants.
(A) Five-week-old plants grown in the growth chamber.
Figure 1A: In the original figure, the images of plants were pasted into a duplicated image of soil in a pot. In the corrected figure, an image of 4-week-old plants is shown without any alteration. The figure legend has been changed accordingly.
Figures 6B and 6C: The background was improperly cloned in the original Figures 6B and 6C. Because the original Figure 6C could not be found, we repeated the experiments and reproduced the corrected Figure 6C, and the results and conclusions were consistent with the original Figure 6C. The corrected Figure 6B was produced from unmanipulated original images. The wild-type samples of 0, 1, 2, and 4 h were analyzed in the same gel but in noncontiguous lanes. The corrected Figure 6B indicates the splicing of these lanes by a black vertical bar. The legend has been changed accordingly for clarity (sentence added highlighted in bold).
Figure 8: In the original Figure 8, actin 20-cycle and 25-cycle control lanes from a single experiment were reused because the RT-PCR analyses of At1g02900, At1g02920, and actin were performed in the same experiment. The figures were rearranged more logically in the corrected Figure 8. The figure legend has been changed accordingly.
Figure 8.
Original: RT-PCR Analysis of Mutant Plants.
RT-PCR was performed with specific primers for At1g02900, At1g02910, and At1g02920 (left gel) and actin-specific primers (right gel).
Figure 10A: This figure contains two images from two independent gels and it has been corrected to indicate the splicing of the wild-type thylakoids and RNC lanes by a black vertical bar. The legend has been changed accordingly for clarity (sentence added highlighted in bold).
Figure 10C: In the original figure, for the bands corresponding to LHCII, the minor LHCII bands were removed and covered by the blank background, and the image was flipped vertically (upside down). The corrected figure was produced from the original immunoblot images. The figure legend has not been changed.
Figure 11: In the corrected figure, several black vertical bars have been inserted between the 10 and 11 lanes to indicate the splicing of separate lanes from the different gels but in the same experiment. The image corresponding to LHCII was flipped vertically (upside down) and cropped too closely to show minor bands. It is shown in the correct orientation in the corrected image. The legend has been changed accordingly for clarity (sentenced added highlighted in bold).
Footnotes
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Editor’s note: The corrected figures and accompanying text were reviewed by members of The Plant Cell editorial board based on the information provided by the authors. The authors are responsible for providing a complete listing and accurate explanations for all known errors or instances of inappropriate data handling or image manipulation associated with the original publication.