Murmu J., Bush M.J., DeLong C., Li S., Xu M., Khan M., Malcolmson C., Fobert P.R., Zachgo S., and Hepworth S.R. Arabidopsis Basic Leucine-Zipper Transcription Factors TGA9 and TGA10 Interact with Floral Glutaredoxins ROXY1 and ROXY2 and Are Redundantly Required for Anther Development.
In Figure 8B in this article, the interaction of TGA9 and TGA10 proteins with ROXY1 and ROXY2 proteins was analyzed by yeast two-hybrid and bimolecular fluorescence complementation studies. For the bimolecular fluorescence complementation analysis (Fig. 8B), three of the four images (YN-ROXY1/YC-TGA10, YN-ROXY2/YC-TGA9, YN-ROXY2/YC-TGA10, blue boxes) were erroneously taken from figure 2 in Li et al. (2009), where PERIANTHIA interactions with other TGA proteins were analyzed. These mistakes have been corrected to show the images demonstrating nuclear interactions for all four protein pairs.
Figure 8.
TGA9 and TGA10 directly interact with ROXY1/2. A, Yeast two-hybrid assay. ROXY proteins served as bait. NPR1 and CRUCIFERIN were negative controls. Error bars indicate se (three replicates). B, ROXY proteins interact with TGA9/10 in the nuclei of transiently transformed tobacco leaves. Images show reconstitution of YFP fluorescence 3 to 5 d after coexpression of protein pairs. The N terminus of YFP (YN) was fused in-frame upstream of ROXY1 and ROXY2. The C terminus of YFP (YC) was fused in-frame upstream of TGA9 and TGA10. As a negative control, coexpression of YN-ROXY1 and YC alone failed to reconstitute a fluorescent YFP chromophore (data not shown). Bars = 50 μm.
The original conclusions of this article are unaffected by the errors made in the figure. The corrected Figure 8 and complete legend appear below. Additional information on Figure 8B also is shown.
References
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