In the article, “Coregulation in human leukocytes of the long pentraxin PTX3 and TSG‐6,” by V. Maina, A. Cotena, A. Doni, M. Nebuloni, F. Pasqualini, C. M. Milner, A. J. Day, A. Mantovani, and C. Garlanda, which appeared in the July 2009 issue of the Journal of Leukocyte Biology (doi:10.1189/jlb.0608345), the legend of Figure 3 has been revised by the authors for clarification. The revised legend is below:
Figure 3: Comparison of TSG‐6 and PTX3 expression in human leukocytes stimulated with pro‐ and anti‐inflammatory stimuli. (A) MoDC were differentiated from monocytes and incubated for 4 h with the stimuli indicated; two different concentrations of LPS were used (1 and 10 ng/ml), where this was added to the cultures after 30 min preincubation with IL‐10, IL‐4 (20 ng/ml) or IFN‐γ (5000 U/ml). After total mRNA extraction, TSG‐6 and PTX3 expression were determined by Northern blot analysis. TGS‐6 expression data are the result of rehybridization of a blot originally hybridized with a PTX3 probe, (reported in ref. [40], and shown here by way of comparison). The lower panels show the ethidium bromide‐stained membranes. The figure shows PTX3 and TSG‐6 expression in MoDC in one donor out of the three tested, where these all gave similar results. (B) hPMN were incubated for 4 h or 20 h with LPS (100 ng/ml), alone or in combination with IFN‐γ (5000 U/ml), IL‐10, IL‐4 or IL‐13 (20 ng/ml), where the latter were added to cultures 30 min prior to LPS. Ctrl, Control.
This has been corrected online.
