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. 2003 Jan 31;32(5):249–270. doi: 10.1046/j.1365-2184.1999.3250249.x

Shemin pathway and peroxidase deficiency in a fully habituated and fully heterotrophic non‐organogenic sugarbeet callus: an adaptative strategy or the consequence of modified hormonal balances and sensitivities in these cancerous cells? A review and reassessment

T Gaspar 1,2, C Kevers 1, B Bisbis 1, C Penel 2, H Greppin 2, F Garnier 1,3, M Rideau 3, C Hault 4, J P Billard 4, J ‐M Foidart 5
PMCID: PMC6726336  PMID: 10619488

Abstract

Abstract. There are many arguments for considering a specific fully habituated (auxin and cytokinin‐independent) and fully heterotrophic non‐organogenic (HNO) sugarbeet callus cell line as terminating a neoplastic progression, and thus to be made of cancerous cells. The similarities with animal tumour and cancer cells are recalled. All types of habituated tissues examined in the literature share at least three common biochemical characteristics: low apparent peroxidase activity, high content of polyamines (PAs) and low production of ethylene. However, results concerning their auxin and cytokinin levels are not consistent. Peroxidase synthesis in the achlorophyllous HNO callus appears to arise from aminolevulinic acid (ALA) synthesis through the Shemin pathway, commonly used by animals and fungi. This pathway is limited by disturbed nitrogen metabolism that diverts glutamate (directly used for ALA synthesis in green higher plants) from the Kreb's cycle into PA synthesis. There is no argument to suggest that the low ethylene production is caused by a competition with PAs for their common precursor, S‐adenosylmethionine. The results we report here indicate modified anabolic and catabolic pathways of auxins and cytokinins but also the possibilities of unusual compounds playing similar roles (dehydrodiconiferyl alcohol glucosides, for instance). A higher turnover of PAs is shown in the HNO callus, which could suggest a role for H2O2 and γ‐aminobutyric acid, products or intermediates in the PA catabolic pathway, as secondary messengers. The habituated cells retain some sensitivity towards exogenous auxins and cytokinins. Their increased sensitivity to PAs and ethylene suggests modified hormonal balances for the control of these actively dividing cells.

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Abbreviations ACC, l‐aminocyclopropane‐1‐carboxylic acid; AIB, α‐aminoisobutyric acid; α‐KG, α‐ketoglutarate; ALA, aminolevulinic acid; AOA, aminooxyacetic acid; DCGs, dehydrodiconiferyl alcohol glucosides; GABA, γ‐aminobutyric acid; GC‐ECD, gas chromatography—electron capture detection; HNO, habituated non‐organogenic; HPLC, high‐performance liquid chromatography; IAA, indoleacetic acid; IAAasp, IAa aspartate; PAs, polyamines; SAM, S‐adenosylmethionine.

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