In the article “Frontline Science: Myeloid cell‐specific deletion of Cebpb decreases sepsis‐induced immunosuppression in mice,” by Melissa B. McPeak, Dima Youssef, Danielle A. Williams, Christopher L. Pritchett, Zhi Q. Yao, Charles E. McCall, Mohamed El Gazzar, appearing in the Journal of Leukocyte Biology, August 2017, 102(2):191‐200; doi: https://doi.org/10.1189/jlb.4HI1216-537R, the authors would like to provide further clarification to Figure 7 with the revised legend:
Figure 7. Adoptive transfer of late sepsis Gr1 + CD11b + cells from C/EBPβ conditional knockout mice into mice undergoing early sepsis enhances the early proinflammatory responses and increases mortality, whereas cells from the control mice exhibit opposite effects. Gr1+CD11b+ cells were isolated from the bone marrow of late septic mice at days 8–16 after CLP using magnetic cell sorting and i.v. injected (4 × 106 cells) into naive (wild‐type) mice that have just underwent CLP. Control mice received 100 μl saline. Because the aim of this experiment was to induce severe sepsis with high mortality so that we could observe the maximal effect of the injected cells, sepsis was induced by 3 punctures, and mice did not receive antibiotic treatment. (A) Survival was recorded for 5 days. Each group included 6 mice. (B) Circulating levels of TNFα and IL‐10. In order to collect blood at specified time points for cytokine measurements, blood was drawn from moribund mice only. Plasma was collected, and levels of TNFα and IL‐10 were determined by ELISA. Data are expressed as mean ± SD (*p < 0.05) of 3 mice per time point pooled from two experiments. These mice are not included in A, which includes mice that died spontaneously (i.e., found dead). *, compared with saline or Gr1+CD11b+ cells from cKO mice. cKO, conditional knockout.
In addition, the size of the needle used for the CLP procedure is stated correctly (23‐gauge) in the legend of Figure 1, but was mistyped (21‐gauge) in the Methods under “Sepsis”. With the Erratum the authors would like to correct the typographic error and clarify that they used a 23‐gauge needle for CLP.
The authors regret any inconvenience caused by this error.
