Abstract
We studied the reaction of rat hippocampal microgliocytes to hyperbaric oxygen at a pressure of 5 ata (absolute atmosphere). Immunohistochemical analysis with selective macrophage marker CD68 (ED1) and microglial marker Iba-1 allowed separate analysis of these two cell populations. It was shown that macrophages do not significantly contribute to reactive changes in the total pool of Iba-1+ hippocampal cells induced by hyperbaric oxygen.
Key Words: hyperbaric oxygen, microglia, macrophages, hippocampus, rat
Footnotes
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 173, No. 5, pp. 626-631, May, 2022
References
- 1.Deng Z, Chen W, Jin J, Zhao J, Xu H. The neuroprotection effect of oxygen therapy: A systematic review and meta-analysis. Niger J. Clin. Pract. 2018;21(4):401–416. doi: 10.4103/njcp.njcp_315_16. [DOI] [PubMed] [Google Scholar]
- 2.Sun L, Zhou W, Mueller C, Sommer C, Heiland S, Bauer AT, Marti HH, Veltkamp R. Oxygen therapy reduces secondary hemorrhage after thrombolysis in thromboembolic cerebral ischemia. J. Cereb. Blood Flow Metab. 2010;30(9):1651–1660. doi: 10.1038/jcbfm.2010.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3.Thiankhaw K, Chattipakorn N, Chattipakorn SC. The effects of hyperbaric oxygen therapy on the brain with middle cerebral artery occlusion. J. Cell. Physiol. 2021;236(3):1677–1694. doi: 10.1002/jcp.29955. [DOI] [PubMed] [Google Scholar]
- 4.Stępień K, Ostrowski RP, Matyja E. Hyperbaric oxygen as an adjunctive therapy in treatment of malignancies, including brain tumours. Med. Oncol. 2016;33(9):101. doi: 10.1007/s12032-016-0814-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5.Kirby J. Hyperbaric oxygen therapy for patients with COVID-19. Emerg. Med. J. 2022;39(2):86–87. doi: 10.1136/emermed-2021-212015. [DOI] [PubMed] [Google Scholar]
- 6.Demchenko IT, Gasier HG, Zhilyaev SY, Moskvin AN, Krivchenko AI, Piantadosi CA, Allen BW. Baroreceptor afferents modulate brain excitation and influence susceptibility to toxic effects of hyperbaric oxygen. J. Appl. Physiol. (1985). 2014;117(5):525-534. doi: 10.1152/japplphysiol.00435.2014 [DOI] [PubMed]
- 7.Koch AE, Kähler W, Wegner-Bröse H, Weyer D, Kuhtz-Buschbeck J, Deuschl G, Eschenfelder CC. Monitoring of CBFV and time characteristics of oxygen-induced acute CNS toxicity in humans. Eur. J. Neurol. 2008;15(7):746–748. doi: 10.1111/j.1468-1331.2008.02158.x. [DOI] [PubMed] [Google Scholar]
- 8.Kobylarek D, Iwanowski P, Lewandowska Z, Limphaibool N, Szafranek S, Labrzycka A, Kozubski W. Advances in the potential biomarkers of epilepsy. Front. Neurol. 2019;10:685. doi: 10.3389/fneur.2019.00685. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Vasilev DS, Tumanova NL, Kim KK, Lavrentyeva VV, Lukomskaya NY, Zhuravin IA, Magazanik LG, Zaitsev AV. Transient morphological alterations in the hippocampus after pentylenetetrazole-induced seizures in rats. Neurochem. Res. 2018;43(8):1671–1682. doi: 10.1007/s11064-018-2583-y. [DOI] [PubMed] [Google Scholar]
- 10.Qiu M, Xu E, Zhan L. Epigenetic regulations of microglia/macrophage polarization in ischemic stroke. Front. Mol. Neurosci. 2021;14:697416. doi: 10.3389/fnmol.2021.697416. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.Xiong XY, Liu L, Yang QW. Functions and mechanisms of microglia/macrophages in neuroinflammation and neurogenesis after stroke. Prog. Neurobiol. 2016;142:23–44. doi: 10.1016/j.pneurobio.2016.05.001. [DOI] [PubMed] [Google Scholar]
- 12.Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates. San Diego, 1998.
- 13.Korzhevskii DE, Kirik OV. Brain microglia and microglial markers. Neurosci. Behav. Physiol. 2016;46(3):284–290. doi: 10.1007/s11055-016-0231-z. [DOI] [Google Scholar]
- 14.Alekseeva OS, Zhilyaev SYu, Platonova TF, Tsyba DL, Kirik OV, Korzhevskii DE, Demchenko IT. Involvement of glutamine synthetase in the development of hyperbaric oxygen seizures. Ross. Fiziol. Zh. 2022;108(1):109-120. Russian. doi: 10.31857/S0869813922010022
- 15.Cevik NG, Orhan N, Yilmaz CU, Arican N, Ahishali B, Kucuk M, Kaya M, Toklu AS. The effects of hyperbaric air and hyperbaric oxygen on blood-brain barrier integrity in rats. Brain Res. 2013;1531:113–121. doi: 10.1016/j.brainres.2013.07.052. [DOI] [PubMed] [Google Scholar]