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. 1980 Feb;39(1):82–86. doi: 10.1136/ard.39.1.82

NK and K cell activity of human blood: differences according to sex, age, and disease.

J Penschow, I R Mackay
PMCID: PMC1000475  PMID: 7377864

Abstract

We report a study on the activity of NK cells ('natural' killer cells) and K cells (antibody-dependent killer cells) in human peripheral blood in health and disease. The 'targets' used were cells of the Chang cell-line, sensitised with rabbit anti-Chang cell antibody for K cell activity, and killing was assessed by release of radiochromium at effector: target ratios of 50:1 and 100:1. The positive findings were that NK cell activity, but not K cell activity, was greater in males and in youth, that NK cell activity was reduced in systemic lupus erythematosus, that neither NK nor K cell activity was altered in rheumatoid arthritis, and that K cell activity was reduced in chronic active hepatitis.

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Selected References

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  1. Altman A., Rapp H. J. Natural cell-mediated cytotoxicity in guinea pigs: properties and specificity of natural killer cells. J Immunol. 1978 Dec;121(6):2244–2252. [PubMed] [Google Scholar]
  2. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  3. Cochrane A. M., Moussouros A., Smith A., Portmann B., Eddleston A. L., Williams R. Lymphocyte cytotoxicity in chronic active hepatitis: effect of therapy and correlations with clinical and histological changes. Gut. 1978 Apr;19(4):308–314. doi: 10.1136/gut.19.4.308. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diaz-Jouanen E., Bankhurst A. D., Williams R. C., Jr Antibody-mediated lymphocytotoxicity in rheumatoid arthritis and systemic lupus erythematosus. Arthritis Rheum. 1976 Mar-Apr;19(2):133–141. doi: 10.1002/art.1780190202. [DOI] [PubMed] [Google Scholar]
  5. Eckhardt R., Kloos P., Dierich M. P., Meyer zum Büschenfelde K. H. K-lymphocytes (killer-cells) in Crohn's disease and acute virus B-hepatitis. Gut. 1977 Dec;18(12):1010–1016. doi: 10.1136/gut.18.12.1010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Herberman R. B., Holden H. T. Natural cell-mediated immunity. Adv Cancer Res. 1978;27:305–377. doi: 10.1016/s0065-230x(08)60936-7. [DOI] [PubMed] [Google Scholar]
  7. Horwitz D. A., Juul-Nielsen K. Human blood L lymphocytes in patients with active systemic lupus erythematosus, rheumatoid arthritis and scleroderma: a comparison with T and B cells. Clin Exp Immunol. 1977 Dec;30(3):370–378. [PMC free article] [PubMed] [Google Scholar]
  8. Isturiz M. A., De Bracco M. M., Pizzi A. M., Manni J. A. Antibody-dependent cell-mediated cytotoxicity in rheumatoid arthritis. Effect of rheumatoid serum fractions on normal lymphocytes. Arthritis Rheum. 1976 Jul-Aug;19(4):725–730. doi: 10.1002/1529-0131(197607/08)19:4<725::aid-art1780190411>3.0.co;2-y. [DOI] [PubMed] [Google Scholar]
  9. Jondal M., Pross H. Surface markers on human b and t lymphocytes. VI. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations. Int J Cancer. 1975 Apr 15;15(4):596–605. doi: 10.1002/ijc.2910150409. [DOI] [PubMed] [Google Scholar]
  10. Kakumu S., Hara T., Goji H., Sakamoto N. Lymphocyte cytotoxicity against Chang liver cells in chronic active hepatitis. Cell Immunol. 1978 Mar 1;36(1):46–53. doi: 10.1016/0008-8749(78)90249-6. [DOI] [PubMed] [Google Scholar]
  11. Kiessling R., Klein E., Wigzell H. "Natural" killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype. Eur J Immunol. 1975 Feb;5(2):112–117. doi: 10.1002/eji.1830050208. [DOI] [PubMed] [Google Scholar]
  12. MacLennan I. C., Loewi G., Howard A. A human serum immunoglobulin with specificity for certain homologous target cells, which induces target cell damage by normal human lymphocytes. Immunology. 1969 Dec;17(6):897–910. [PMC free article] [PubMed] [Google Scholar]
  13. McGill P. E., Twinn I. Antibody-mediated cytotoxicity in rheumatoid arthritis. Ann Rheum Dis. 1977 Jun;36(3):268–270. doi: 10.1136/ard.36.3.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morris P. J., Vaughan H., Tait B. D., Mackay I. R. Histocompatibility antigens (HLA): associations with immunopathic diseases and with responses to microbial antigens. Aust N Z J Med. 1977 Dec;7(6):616–624. doi: 10.1111/j.1445-5994.1977.tb02318.x. [DOI] [PubMed] [Google Scholar]
  15. Panayi G. S., Corrigall V. Functional assay of cytotoxic lymphocytes involved in antibody-mediated cytotoxicity in normal and rheumatoid subjects. Ann Rheum Dis. 1977 Jun;36(3):257–260. doi: 10.1136/ard.36.3.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
  17. Perlmann P., Perlmann H., Larsson A., Wåhlin B. Antibody dependent cytolytic effector lymphocytes (K cells) in human blood. J Reticuloendothel Soc. 1975 Apr;17(4):241–250. [PubMed] [Google Scholar]
  18. Petrányi G. G., Benczur M., Onody C. E., Hollán S. R. Letter: HL-A 3,7 and lymphocyte cytotoxic activity. Lancet. 1974 Apr 20;1(7860):736–736. doi: 10.1016/s0140-6736(74)92943-2. [DOI] [PubMed] [Google Scholar]
  19. Roder J. C., Kiessling R. Target--effector interaction in the natural killer cell system. I. Covariance and genetic control of cytolytic and target-cell-binding subpopulations in the mouse. Scand J Immunol. 1978;8(2):135–144. doi: 10.1111/j.1365-3083.1978.tb00505.x. [DOI] [PubMed] [Google Scholar]
  20. Rosenberg E. B., McCoy J. L., Green S. S., Donnelly F. C., Siwarski D. F., Levine P. H., Herberman R. B. Destruction of human lymphoid tissue-culture cell lines by human peripheral lymphocytes in 51Cr-release cellular cytotoxicity assays. J Natl Cancer Inst. 1974 Feb;52(2):345–352. doi: 10.1093/jnci/52.2.345. [DOI] [PubMed] [Google Scholar]
  21. Santoli D., Trinchieri G., Moretta L., Zmijewski C. M., Koprowski H. Spontaneous cell-mediated cytotoxicity in humans. Distribution and characterization of the effector cell. Clin Exp Immunol. 1978 Aug;33(2):309–318. [PMC free article] [PubMed] [Google Scholar]
  22. Santoli D., Trinchieri G., Zmijewski C. M., Koprowski H. HLA-related control of spontaneous and antibody-dependent cell-mediated cytotoxic activity in humans. J Immunol. 1976 Sep;117(3):765–770. [PubMed] [Google Scholar]
  23. Schneider J., Chin W., Friou G. J., Cooper S. M., Harding B., Hill R. L., Quismorio F. P. Reduced antibody-dependent cell-mediated cytotoxicity in systemic lupus erythematosus. Clin Exp Immunol. 1975 May;20(2):187–192. [PMC free article] [PubMed] [Google Scholar]
  24. Toh B. H., Roberts-Thomson I. C., Mathews J. D., Whittingham S., Mackay I. R. Depression of cell-mediated immunity in old age and the immunopathic diseases, lupus erythematosus, chronic hepatitis and rheumatoid arthritis. Clin Exp Immunol. 1973 Jun;14(2):193–202. [PMC free article] [PubMed] [Google Scholar]
  25. Vierling J. M., Nelson D. L., Strober W., Bundy D. M., Jones E. A. In vitro cell-mediated cytotoxicity in primary biliary cirrhosis and chronic hepatitis. Dysfunction of spontaneous cell-mediated cytotoxicity in primary biliary cirrhosis. J Clin Invest. 1977 Nov;60(5):1116–1128. doi: 10.1172/JCI108863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. West W. H., Cannon G. B., Kay H. D., Bonnard G. D., Herberman R. B. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. J Immunol. 1977 Jan;118(1):355–361. [PubMed] [Google Scholar]
  27. Whittingham S., Pitt D. B., Sharma D. L., Mackay I. R. Stress deficiency of the T-lymphocyte system exemplified by Down syndrome. Lancet. 1977 Jan 22;1(8004):163–166. doi: 10.1016/s0140-6736(77)91763-9. [DOI] [PubMed] [Google Scholar]

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