Skip to main content
NCBI home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1980 Jun;39(3):285–290. doi: 10.1136/ard.39.3.285

Native type II collagen-induced arthritis in the rat. I. Incidence and humoral response to collagen.

K Morgan, R B Clague, M J Shaw, P J Holt
PMCID: PMC1000533  PMID: 7416820

Abstract

An acute inflammatory arthritis has been induced in 76% of rats injected intradermally with native bovine type II collagen emulsified in Freund's complete (CFA) or incomplete (ICFA) adjuvant. The arthritis became chronic in 14 out of 31 rats, and ear and tail lesions were noted in some rats. No arthritis was induced by native type I collagen, denatured type II collagen, rabbit IgG, or buffer alone injected intradermally with adjuvant. Using a solid-phase radioimmunoassay for serum antibodies we have shown that IgM and IgG levels to native bovine type II collagen were significantly higher in arthritic than nonarthritic rats. Antiglobulin antibody levels were not raised in arthritic rats. Equivalent antibody levels to native type II collgen were obtained whether this antigen was emulsified in ICFA or CFA. However, native type I collagen produced high antibody levels only when emulsified in CFA, indicating a difference in the immunogenicity of these collagens. These studies suggest that native type II collagen possesses arthritogenic properties in the rat and that humoral immunity may play a role in the induction of this arthritis.

Full text

PDF
285

Images in this article

286Image
on p.286

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andriopoulos N. A., Mestecky J., Miller E. J., Bradley E. L. Antibodies to native and denatured collagens in sera of patients with rheumatoid arthritis. Arthritis Rheum. 1976 May-Jun;19(3):613–617. doi: 10.1002/art.1780190314. [DOI] [PubMed] [Google Scholar]
  2. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  3. Brackertz D., Mitchell G. F., Mackay I. R. Antigen-induced arthritis in mice. I. Induction of arthritis in various strains of mice. Arthritis Rheum. 1977 Apr;20(3):841–850. doi: 10.1002/art.1780200314. [DOI] [PubMed] [Google Scholar]
  4. Brackertz D., Mitchell G. F., Vadas M. A., Mackay I. R., Miller J. F. Studies on antigen-induced arthritis in mice. II. Immunologic correlates of arthritis susceptibility in mice. J Immunol. 1977 May;118(5):1639–1644. [PubMed] [Google Scholar]
  5. Cooke T. D., Jasin H. E. The pathogenesis of chronic inflammation in experimental antigen-induced arthritis. I. The role of antigen on the local immune response. Arthritis Rheum. 1972 Jul-Aug;15(4):327–337. doi: 10.1002/art.1780150402. [DOI] [PubMed] [Google Scholar]
  6. DUMONDE D. C., GLYNN L. E. The production of arthritis in rabbits by an immunological reaction to fibrin. Br J Exp Pathol. 1962 Aug;43:373–383. [PMC free article] [PubMed] [Google Scholar]
  7. Eyre D. R., Muir H. Types I and II collagens in intervertebral disc. Interchanging radial distributions in annulus fibrosus. Biochem J. 1976 Jul 1;157(1):267–270. doi: 10.1042/bj1570267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Furthmayr H., Timpl R. Immunochemistry of collagens and procollagens. Int Rev Connect Tissue Res. 1976;7:61–99. doi: 10.1016/b978-0-12-363707-9.50008-3. [DOI] [PubMed] [Google Scholar]
  9. Hay F. C., Nineham L. J., Roitt I. M. Routine assay for detection of IgG and IgM antiglobulins in seronegative and seropositive rheumatoid arthritis. Br Med J. 1975 Jul 26;3(5977):203–204. doi: 10.1136/bmj.3.5977.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miller E. J., Matukas V. J. Biosynthesis of collagen. The biochemist's view. Fed Proc. 1974 May;33(5):1197–1204. [PubMed] [Google Scholar]
  11. Miller E. J. Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin. Biochemistry. 1972 Dec 19;11(26):4903–4909. doi: 10.1021/bi00776a005. [DOI] [PubMed] [Google Scholar]
  12. PEARSON C. M., WOOD F. D. Studies of arthritis and other lesions induced in rats by the injection of mycobacterial adjuvant. VII. Pathologic details of the arthritis and spondylitis. Am J Pathol. 1963 Jan;42:73–95. [PMC free article] [PubMed] [Google Scholar]
  13. Trelstad R. L., Rubin D., Gross J. Osteogenesis imperfecta congenita: evidence for a generalized molecular disorder of collagen. Lab Invest. 1977 May;36(5):501–508. [PubMed] [Google Scholar]
  14. Trentham D. E., Townes A. S., Kang A. H. Autoimmunity to type II collagen an experimental model of arthritis. J Exp Med. 1977 Sep 1;146(3):857–868. doi: 10.1084/jem.146.3.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Trentham D. E., Townes A. S., Kang A. H., David J. R. Humoral and cellular sensitivity to collagen in type II collagen-induced arthritis in rats. J Clin Invest. 1978 Jan;61(1):89–96. doi: 10.1172/JCI108929. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES