Abstract
Plasma from patients with rheumatoid arthritis shows inhibitory activity towards the random and stimulated movement of human polymorphonuclear leucocytes and peripheral phagocytic monocytes in vitro. The inhibitory activity is heat-labile, not present in plasma from patients with noninflammatory diseases, and appears to exert its inhibitory effects by occupying sites on the cell cell membrane from where it may be removed by washing. It is suggested that this factor(s) is responsible for the diminished accumulation of polymorphonuclear leucocytes into skin chambers with occurs in patients with rheumatoid arthritis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ferrante A., Thong Y. H. A rapid one-step procedure for purification of mononuclear and polymorphonuclear leukocytes from human blood using a modification of the Hypaque-Ficoll technique. J Immunol Methods. 1978;24(3-4):389–393. doi: 10.1016/0022-1759(78)90143-6. [DOI] [PubMed] [Google Scholar]
- Keller H. U., Wilkinson P. C., Abercrombie M., Becker E. L., Hirsch J. G., Miller M. E., Ramsey W. S., Zigmond S. H. A proposal for the definition of terms related to locomotion of leukocytes and other cells. J Immunol. 1977 May;118(5):1912–1914. [PubMed] [Google Scholar]
- Mowat A. G., Baum J. Chemotaxis of polymorphonuclear leukocytes from patients with rheumatoid arthritis. J Clin Invest. 1971 Dec;50(12):2541–2549. doi: 10.1172/JCI106754. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson R. D., Quie P. G., Simmons R. L. Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes. J Immunol. 1975 Dec;115(6):1650–1656. [PubMed] [Google Scholar]
- Repine J. E., Clawson C. C. Influence of surface proteins and separation techniques on neutrophil unstimulated and stimulated locomotion in vitro. J Reticuloendothel Soc. 1978 Sep;24(3):217–226. [PubMed] [Google Scholar]
- Walker J. R., James D. W., Smith M. J. Directed migration of circulating polymorphonuclear leucocytes in patients with rheumatoid arthritis: a defect in the plasma. Ann Rheum Dis. 1979 Jun;38(3):215–218. doi: 10.1136/ard.38.3.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Walker J. R., Smith M. J., James D. W. A comparison of two in vitro methods for studying a defect in leucocyte movement in rheumatoid arthritis. Int Arch Allergy Appl Immunol. 1979;59(3):343–348. doi: 10.1159/000232279. [DOI] [PubMed] [Google Scholar]