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. 1981 Feb;40(1):60–63. doi: 10.1136/ard.40.1.60

In-vitro T cell mediated function in patients with active rheumatoid arthritis.

S Slavin, S Strober
PMCID: PMC1000657  PMID: 6970551

Abstract

In-vitro synthesis of peripheral blood lymphocytes from patients with rheumatoid arthritis was measured after stimulation with phytohaemagglutinin (PHA) in a short-term, serum-free culture system. Diminished responses were found in 16 out of 17 consecutive patients with active disease. Normal PHA responsiveness was recovered by assaying Ficoll-Hypaque isolated E rosette forming cells in serum-free medium, indicating basically normal T cell function in RA. Preincubation of normal peripheral blood lymphocytes (or isolated E rosette forming cells) with sera obtained from patients with active RA for 30 minutes at 4 degrees C or 37 degrees C blocked PHA responsiveness in 34 out of 43 tests. This suggests that serum blocking factors may be responsible for reduced T cell reactivity in RA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bentwich Z., Douglas S. D., Siegal F. P., Kunkel H. G. Human lymphocyte-sheep erythrocyte rosette formation: some characteristics of the interaction. Clin Immunol Immunopathol. 1973 Jul;1(4):511–522. doi: 10.1016/0090-1229(73)90007-x. [DOI] [PubMed] [Google Scholar]
  2. Bobrove A. M., Strober S., Herzenberg L. A., DePamphilis J. D. Identification and quantitation of thymus-derived lymphocytes in human peripheral blood. J Immunol. 1974 Feb;112(2):520–527. [PubMed] [Google Scholar]
  3. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  4. Lance E. M., Knight S. C. Immunologic reactivity in rheumatoid arthritis. Response to mitogens. Arthritis Rheum. 1974 Sep-Oct;17(5):513–520. doi: 10.1002/art.1780170504. [DOI] [PubMed] [Google Scholar]
  5. Levy R., Kaplan H. S. Impaired lymphocyte function in untreated Hodgkin's disease. N Engl J Med. 1974 Jan 24;290(4):181–186. doi: 10.1056/NEJM197401242900402. [DOI] [PubMed] [Google Scholar]
  6. Lockshin M. D., Eisenhauer A. C., Kohn R., Weksler M., Block S., Mushlin S. B. Cell-mediated immunity in rheumatic diseases. II. Mitogen responses in RA, SLE, and other illnesses: correlation with T- and B-lymphocyte populations. Arthritis Rheum. 1975 May-Jun;18(3):245–250. doi: 10.1002/art.1780180308. [DOI] [PubMed] [Google Scholar]
  7. Reynolds M. D., Abdou N. I. Comparative study of the in vitro proliferative responses of blood and synovial fluid leukocytes of rheumatoid arthritis patients. J Clin Invest. 1973 Jul;52(7):1627–1631. doi: 10.1172/JCI107341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Silverman H. A., Johnson J. S., Vaughan J. H., McGlamory J. C. Altered lymphocyte reactivity in rheumatoid arthritis. Arthritis Rheum. 1976 May-Jun;19(3):509–515. doi: 10.1002/art.1780190301. [DOI] [PubMed] [Google Scholar]
  9. Waxman J., Lockshin M. D., Schnapp J. J., Doneson I. N. Cellular immunity in rheumatic diseases. I. Rheumatoid arthritis. Arthritis Rheum. 1973 Jul-Aug;16(4):499–506. doi: 10.1002/art.1780160410. [DOI] [PubMed] [Google Scholar]
  10. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

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