Skip to main content
PMC home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1983 Apr;42(2):147–150. doi: 10.1136/ard.42.2.147

Complement-activating rheumatoid-factor-containing complexes in patients with rheumatoid vasculitis.

C J Elson, D G Scott, D R Blake, P A Bacon, P D Holt
PMCID: PMC1001088  PMID: 6601932

Abstract

The role of complement and rheumatoid factor in immune complexes was examined in patients with a variety of rheumatic diseases. This was done by assessing the amount of rheumatoid factor (RF) bound from sera by F(ab)2 anti-C3 attached to a solid matrix. High levels of RF bound to C3 were detected in patients with rheumatoid arthritis complicated by vasculitis but rarely and in lower levels in patients with synovitis, ankylosing spondylitis, and systemic lupus erythematosus. The activity was bound to anti-C3 through anti-C3 antibodies because little was bound by normal F(ab)2 and was evidently complexed in the sera before in-vitro testing, since it was precipitated by 2 . 5% polyethylene glycol and sedimented with high molecular weight material on sucrose density gradient ultracentrifugation. It is considered that RF-containing complexes are present in vasculitic sera and have the potential to bind complement in vivo.

Full text

PDF
147

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen C., Elson C. J., Scott D. G., Bacon P. A., Bucknall R. C. IgG antiglobulins in rheumatoid arthritis and other arthritides: relationship with clinical features and other parameters. Ann Rheum Dis. 1981 Apr;40(2):127–131. doi: 10.1136/ard.40.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cochrane C. G., Hawkins D. Studies on circulating immune complexes. 3. Factors governing the ability of circulating complexes to localize in blood vessels. J Exp Med. 1968 Jan 1;127(1):137–154. doi: 10.1084/jem.127.1.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Creighton W. D., Lambert P. H., Miescher P. A. Detection of antibodies and soluble antigen-antibody complexes by precipitation with polyethylene glycol. J Immunol. 1973 Oct;111(4):1219–1227. [PubMed] [Google Scholar]
  4. Freedman J., Mollison P. L. Preparation of red cells coated with C4 and C3 subcomponents and production of anti-C4d and anti-C3d. Vox Sang. 1976 Oct;31(4):241–257. doi: 10.1111/j.1423-0410.1976.tb04647.x. [DOI] [PubMed] [Google Scholar]
  5. Fruitstone M. J. C3b-sensitized erythrocytes. Transfusion. 1978 Jan-Feb;18(1):125–125. doi: 10.1046/j.1537-2995.1978.18178118556.x. [DOI] [PubMed] [Google Scholar]
  6. Jones V. E., Cowley P. J., Allen C., Elson C. J. The isolation of immune complexes containing IgM rheumatoid factor and recovery of IgG rheumatoid factor from the complexes. J Immunol Methods. 1980;37(1):1–14. doi: 10.1016/0022-1759(80)90176-3. [DOI] [PubMed] [Google Scholar]
  7. Morgenthaler J. J., Baillod P., Friedli H. Fatty acid patterns during plasma fractionation. Vox Sang. 1980;38(5):288–293. doi: 10.1111/j.1423-0410.1980.tb02369.x. [DOI] [PubMed] [Google Scholar]
  8. Scott D. G., Bacon P. A., Allen C., Elson C. J., Wallington T. IgG rheumatoid factor, complement and immune complexes in rheumatoid synovitis and vasculitis: comparative and serial studies during cytotoxic therapy. Clin Exp Immunol. 1981 Jan;43(1):54–63. [PMC free article] [PubMed] [Google Scholar]
  9. Stratton F., Rawlinson V. I. Preparation of test cells for the antiglobulin test. J Clin Pathol. 1974 May;27(5):359–367. doi: 10.1136/jcp.27.5.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wedmore C. V., Williams T. J. Control of vascular permeability by polymorphonuclear leukocytes in inflammation. Nature. 1981 Feb 19;289(5799):646–650. doi: 10.1038/289646a0. [DOI] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES