Skip to main content
NCBI home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1983 Apr;42(2):187–191. doi: 10.1136/ard.42.2.187

Effects of prostaglandins E1, E2, and F2 alpha on N-acetyl-beta-glucosaminidase activities of human synovial cells in culture.

B J Clarris, L P Malcolm
PMCID: PMC1001097  PMID: 6573873

Abstract

The acid hydrolase N-acetyl-beta-glucosaminidase (NAG) was used to examine the effects of prostaglandins E1 (PGE1), E2 (PGE2), and F2 alpha (PGF2 alpha) on the lysosomal system of human synovial cells in vitro. A spontaneous release of the enzyme occurred from control cultures, which was accelerated by each of the prostaglandins in a concentration-dependent manner, within the range of 10(-8)-10(-6) moles per litre (M). No clear order of potency could be established. The effects of the prostaglandins on release of NAG were less consistent and of smaller magnitude when human serum was replaced by bovine serum albumin in the medium. In the presence of serum small increases also occurred in intracellular NAG activity, but only the effect of PGE1 was statistically significant. The prostaglandins did not appreciably affect the previously established pattern of increased intracellular activity of NAG and reduced release produced by sucrose.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARLAND P., NOVIKOFF A. B., HAMERMAN D. Electron microscopy of the human synovial membrane. J Cell Biol. 1962 Aug;14:207–220. doi: 10.1083/jcb.14.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bockman R. S. Prostaglandin production by human blood monocytes and mouse peritoneal macrophages: synthesis dependent on in vitro culture conditions. Prostaglandins. 1981 Jan;21(1):9–31. doi: 10.1016/0090-6980(81)90192-1. [DOI] [PubMed] [Google Scholar]
  3. Burleigh M. C., Barrett A. J., Lazarus G. S. Cathepsin B1. A lysosomal enzyme that degrades native collagen. Biochem J. 1974 Feb;137(2):387–398. doi: 10.1042/bj1370387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Castor C. W. Connective tissue activation. VII. Evidence supporting a role for prostaglandins and cyclic nucleotides. J Lab Clin Med. 1975 Mar;85(3):392–404. [PubMed] [Google Scholar]
  5. Clarris B. J., Fraser J. R. The effects of homologous and heterologous whole serum upon multiplication of recently-isolated human synovial cells in culture. Aust J Exp Biol Med Sci. 1967 Oct;45(5):549–560. doi: 10.1038/icb.1967.54. [DOI] [PubMed] [Google Scholar]
  6. Clarris B. J. Morphological effects of prostaglandins E1, E2 and F2 alpha on fibroblast-like cultures of human synovial cells. Experientia. 1982 Mar 15;38(3):350–351. doi: 10.1007/BF01949386. [DOI] [PubMed] [Google Scholar]
  7. Dayer J. M., Krane S. M., Russell R. G., Robinson D. R. Production of collagenase and prostaglandins by isolated adherent rheumatoid synovial cells. Proc Natl Acad Sci U S A. 1976 Mar;73(3):945–949. doi: 10.1073/pnas.73.3.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eeckhout Y., Vaes G. Further studies on the activation of procollagenase, the latent precursor of bone collagenase. Effects of lysosomal cathepsin B, plasmin and kallikrein, and spontaneous activation. Biochem J. 1977 Jul 15;166(1):21–31. doi: 10.1042/bj1660021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Egg D., Günther R., Herold M., Kerschbaumer F. Prostaglandin E2 und F2 alpha Konzentrationen in der Synovia bei rheumatischen und traumatischen Kniegelenkerkrankungen. Z Rheumatol. 1980 May-Jun;39(5-6):170–175. [PubMed] [Google Scholar]
  10. FRASER J. R., CATT K. J. Human synovial-cell culture use of a new method in a study of rheumatoid arthritis. Lancet. 1961 Dec 30;2(7218):1437–1439. doi: 10.1016/s0140-6736(61)91252-1. [DOI] [PubMed] [Google Scholar]
  11. Fraser J. R., Clarris B. J., Baxter E. Patterns of induced variation in the morphology, hyaluronic acid secretion, and lysosomal enzyme activity of cultured human synovial cells. Ann Rheum Dis. 1979 Jun;38(3):287–294. doi: 10.1136/ard.38.3.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fraser J. R., McCall J. F. Culture of synovial cells in vitro. Notes on isolation and propagation. Ann Rheum Dis. 1965 Jul;24(4):351–359. doi: 10.1136/ard.24.4.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Marshall J. L., Fraser J. R., Muirden K. D. Lysosomal activation by neutral saccharides in cell cultures of synovium. Ann Rheum Dis. 1977 Apr;36(2):130–138. doi: 10.1136/ard.36.2.130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oropeza-Rendon R. L., Speth V., Fischer H., Quintart J., Baudhuin P. Action of prostaglandin E1 and F2 alpha on the pinocytic activity of horseradish peroxidase in bone-marrow derived macrophages. Eur J Cell Biol. 1980 Oct;22(2):761–765. [PubMed] [Google Scholar]
  15. Robinson D. R., McGuire M. B., Levine L. Prostaglandins in the rheumatic diseases. Ann N Y Acad Sci. 1975 Jun 13;256:318–329. doi: 10.1111/j.1749-6632.1975.tb36058.x. [DOI] [PubMed] [Google Scholar]
  16. Robinson D. R., Tashjian A. H., Jr, Levine L. Prostaglandin-stimulated bone resorption by rheumatoid synovia. A possible mechanism for bone destruction in rheumatoid arthritis. J Clin Invest. 1975 Nov;56(5):1181–1188. doi: 10.1172/JCI108195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rouzer C. A., Scott W. A., Kempe J., Cohn Z. A. Prostaglandin synthesis by macrophages requires a specific receptor-ligand interaction. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4279–4282. doi: 10.1073/pnas.77.7.4279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Roy S., Ghadially F. N., Crane W. A. Synovial membrane in traumatic effusion. Ultrastructure and autoradiography with tritiated leucine. Ann Rheum Dis. 1966 May;25(3):259–271. doi: 10.1136/ard.25.3.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weidemann M. J., Peskar B. A., Wrogemann K., Rietschel E. T., Staudinger H., Fischer H. Prostaglandin and thromboxane synthesis in a pure macrophage population and the inhibition, by E-type prostaglandins, of chemiluminescence. FEBS Lett. 1978 May 1;89(1):136–140. doi: 10.1016/0014-5793(78)80539-0. [DOI] [PubMed] [Google Scholar]
  20. Zurier R. B., Hoffstein S., Weissmann G. Mechanisms of lysosomal enzyme release from human leukocytes. I. Effect of cyclic nucleotides and colchicine. J Cell Biol. 1973 Jul;58(1):27–41. doi: 10.1083/jcb.58.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES