Skip to main content
NCBI home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1984 Feb;43(1):83–90. doi: 10.1136/ard.43.1.83

Biomechanical and biochemical properties of dog cartilage in experimentally induced osteoarthritis.

R D Altman, J Tenenbaum, L Latta, W Riskin, L N Blanco, D S Howell
PMCID: PMC1001229  PMID: 6696526

Abstract

The finding of other investigators that increased water content is often associated with signs of a torn collagen network in human osteoarthritic (OA) cartilage led to this study. In the Pond-Nuki model of post-traumatic OA experimental but not control femoral condylar cartilage showed evidence of breakdown and stiffening of collagen network as assessed by measurement of swelling properties and indentation behaviour respectively. These changes in the unstable knees occurred despite lack of erosion of that surface cartilage ascertained from carbon black mapping and history. The stiffening rather than softening change was therefore attributed to cartilage oedema of the middle and deep certilagenous zones, wherein breakdown of collagen network has been postulated to occur. Because of insignificant reduction of total hexuronate in these cartilages, a proteoglycan (PG) profile of sedimentation coefficients for aggregate (PGA) and subunit species (PGS) was analysed to see if collagen network changes in the dog preceded PG alteration. Despite minimal histological changes our results confirmed previous findings in the tibial plateau cartilage in this model, that PGA was reduced in size and PGS increased in amount. Slight enzymatic breakdown of PGs, or altered synthesis due to cellular responses to either the injury directly or to synovial inflammation, seems necessary to explain such changes in the absence of cartilage erosion.

Full text

PDF
83

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altman R. D., Pita J. C., Howell D. S. Degradation of proteoglycans in human osteoarthritic cartilage. Arthritis Rheum. 1973 Mar-Apr;16(2):179–185. doi: 10.1002/art.1780160207. [DOI] [PubMed] [Google Scholar]
  2. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  3. Bollet A. J., Nance J. L. Biochemical Findings in Normal and Osteoarthritic Articular Cartilage. II. Chondroitin Sulfate Concentration and Chain Length, Water, and Ash Content. J Clin Invest. 1966 Jul;45(7):1170–1177. doi: 10.1172/JCI105423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brandt K. D. Enhanced extractability of articular cartilage protoglycans in osteoarthrosis. Biochem J. 1974 Nov;143(2):475–478. doi: 10.1042/bj1430475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gilbertson E. M. Development of periarticular osteophytes in experimentally induced osteoarthritis in the dog. A study using microradiographic, microangiographic, and fluorescent bone-labelling techniques. Ann Rheum Dis. 1975 Feb;34(1):12–25. doi: 10.1136/ard.34.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hascall V. C., Heinegård D. Aggregation of cartilage proteoglycans. I. The role of hyaluronic acid. J Biol Chem. 1974 Jul 10;249(13):4232–4241. [PubMed] [Google Scholar]
  7. Kempson G. E., Spivey C. J., Swanson S. A., Freeman M. A. Patterns of cartilage stiffness on normal and degenerate human femoral heads. J Biomech. 1971 Dec;4(6):597–609. doi: 10.1016/0021-9290(71)90049-2. [DOI] [PubMed] [Google Scholar]
  8. Lemperg R., Larsson S. E., Hjertquist S. O. The glycosaminoglycans of bovine articular cartilage. I. Concentration and distribution in different layers in relation to age. Calcif Tissue Res. 1974;15(3):237–251. doi: 10.1007/BF02059060. [DOI] [PubMed] [Google Scholar]
  9. Mankin H. J., Thrasher A. Z. Water content and binding in normal and osteoarthritic human cartilage. J Bone Joint Surg Am. 1975 Jan;57(1):76–80. [PubMed] [Google Scholar]
  10. Maroudas A., Venn M. Chemical composition and swelling of normal and osteoarthrotic femoral head cartilage. II. Swelling. Ann Rheum Dis. 1977 Oct;36(5):399–406. doi: 10.1136/ard.36.5.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McDevitt C. A., Muir H. Biochemical changes in the cartilage of the knee in experimental and natural osteoarthritis in the dog. J Bone Joint Surg Br. 1976 Feb;58(1):94–101. doi: 10.1302/0301-620X.58B1.131804. [DOI] [PubMed] [Google Scholar]
  12. Meachim G. Light microscopy of Indian ink preparations of fibrillated cartilage. Ann Rheum Dis. 1972 Nov;31(6):457–464. doi: 10.1136/ard.31.6.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moskowitz R. W., Howell D. S., Goldberg V. M., Muniz O., Pita J. C. Cartilage proteoglycan alterations in an experimentally induced model of rabbit osteoarthritis. Arthritis Rheum. 1979 Feb;22(2):155–163. doi: 10.1002/art.1780220208. [DOI] [PubMed] [Google Scholar]
  14. Mow V. C. Biphasic rheological properties of cartilage [proceedings]. Bull Hosp Joint Dis. 1977 Oct;38(2):121–124. [PubMed] [Google Scholar]
  15. Parsons J. R., Black J. Mechanical behavior of articular cartilage: quantitative changes with alteration of ionic environment. J Biomech. 1979;12(10):765–773. doi: 10.1016/0021-9290(79)90162-3. [DOI] [PubMed] [Google Scholar]
  16. Pearson J. P., Mason R. M. The stability of bovine nasal cartilage proteoglycans during isolation and storage. Biochim Biophys Acta. 1977 Jun 23;498(1):176–188. doi: 10.1016/0304-4165(77)90098-8. [DOI] [PubMed] [Google Scholar]
  17. Pita J. C., Muller F. J., Oegema T., Hascall V. C. Determination os sedimentation coefficient distributions for cartilage proteoglycans. Arch Biochem Biophys. 1978 Feb;186(1):66–76. doi: 10.1016/0003-9861(78)90464-2. [DOI] [PubMed] [Google Scholar]
  18. Pita J. C., Müller F. J. Ultracentrifugal study of polydisperse and paucidisperse biological systems using capillary microcells. Biochemistry. 1973 Jul 3;12(14):2656–2665. doi: 10.1021/bi00738a017. [DOI] [PubMed] [Google Scholar]
  19. Pond M. J., Nuki G. Experimentally-induced osteoarthritis in the dog. Ann Rheum Dis. 1973 Jul;32(4):387–388. doi: 10.1136/ard.32.4.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sajdera S. W., Hascall V. C. Proteinpolysaccharide complex from bovine nasal cartilage. A comparison of low and high shear extraction procedures. J Biol Chem. 1969 Jan 10;244(1):77–87. [PubMed] [Google Scholar]
  21. Wu H. C., Yao R. F. Mechanical behavior of the human annulus fibrosus. J Biomech. 1976;9(1):1–7. doi: 10.1016/0021-9290(76)90132-9. [DOI] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES