Figure 6.

Initial and time evolution of autophagy rates depend on rapamycin concentration. (A) Temporal dynamics of R₁ for different concentrations of rapamycin. Data points represent the mean while shaded area represents ± standard deviation. Four independent replicates were performed. (B) R₁ at 10 min after rapamycin addition is plotted as a function of rapamycin concentration. Individual data points represent experimental data while the dotted line represents the model fit. The model used for fitting the data along with the adjusted R² value is also shown. (C) Statistical comparison of R₁ for three different rapamycin concentrations at three different time points. (*) indicates p-value <0.05 and NS indicates not significant. P-values were calculated using an independent two-tail t-test. (D) Temporal evolution of all autophagic rates (R₁, R₂, R₃) for 0.5 nM rapamycin-treated cells. Data points represent the mean while the shaded area represents ± standard deviation. (E) R₁ at 15 h as a function of rapamycin concentration. NS indicates not significant. P-values were calculated using a one-way ANOVA test. (F) AUC for R₃ data for different concentrations of rapamycin. Data represent mean ± standard deviation. P-values were calculated using a one-way ANOVA test followed by Tukey’s post hoc test for pairwise comparison. At least 150–200 cells were imaged for all experiments.