Skip to main content
NCBI home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1984 Oct;43(5):710–715. doi: 10.1136/ard.43.5.710

The presence of abnormal lysosomes in lymphocytes and neutrophils during chloroquine therapy: a quantitative ultrastructural study.

C J Jones, R S Salisbury, M I Jayson
PMCID: PMC1001513  PMID: 6093716

Abstract

Lysosomal ultrastructure in circulating lymphocytes and neutrophils from the blood of 22 patients with rheumatoid arthritis or systemic lupus erythematosus undergoing chloroquine therapy has been examined and compared with that of rheumatoid cases receiving alternative forms of treatment and non-rheumatoid controls. The lymphocytes of all 22 patients receiving chloroquine showed a highly significant number of lamellar inclusion bodies (mean 9.3%) compared with only occasional inclusions in six of the 14 rheumatoid and four of the 12 non-rheumatoid controls (means 0.86% and 0.83% respectively). Neutrophils from eight chloroquine-treated patients also showed abnormal granules which were absent from both control groups. These findings confirm our earlier in vitro work which indicated that lymphocytes, compared with neutrophils, had a greater susceptibility to chloroquine action with respect to alterations in lysosomal structure.

Full text

PDF
710

Images in this article

712Image
on p.712
713Image
on p.713

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLISON A. C., YOUNG M. R. UPTAKE OF DYES AND DRUGS BY LIVING CELLS IN CULTURE. Life Sci. 1964 Dec;3:1407–1414. doi: 10.1016/0024-3205(64)90082-7. [DOI] [PubMed] [Google Scholar]
  2. Abraham R., Hendy R., Grasso P. Formation of myeloid bodies in rat liver lysosomes after chloroquine administration. Exp Mol Pathol. 1968 Oct;9(2):212–229. doi: 10.1016/0014-4800(68)90037-3. [DOI] [PubMed] [Google Scholar]
  3. Fedorko M. E. Effect of chloroquine on morphology of leukocytes and pancreatic exocrine cells from the rat. Lab Invest. 1968 Jan;18(1):27–37. [PubMed] [Google Scholar]
  4. Fedorko M. Effect of chloroquine on morphology of cytoplasmic granules in maturing human leukocytes--an ultrastructural study. J Clin Invest. 1967 Dec;46(12):1932–1942. doi: 10.1172/JCI105683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jones C. J., Jayson M. I. Chloroquine: its effect on leucocyte auto- and heterophagocytosis. Ann Rheum Dis. 1984 Apr;43(2):205–212. doi: 10.1136/ard.43.2.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jones C. J., Morris K. J., Jayson M. I. Prednisolone inhibits phagocytosis by polymorphonuclear leucocytes via steroid receptor mediated events. Ann Rheum Dis. 1983 Feb;42(1):56–62. doi: 10.1136/ard.42.1.56. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Matsuzawa Y., Hostetler K. Y. Inhibition of lysosomal phospholipase A and phospholipase C by chloroquine and 4,4'-bis(diethylaminoethoxy) alpha, beta-diethyldiphenylethane. J Biol Chem. 1980 Jun 10;255(11):5190–5194. [PubMed] [Google Scholar]
  8. Panayi G. S., Neill W. A., Duthie J. J., McCormick J. N. Action of chloroquine phosphate in rheumatoid arthritis. 1. Immunosuppressive effect. Ann Rheum Dis. 1973 Jul;32(4):316–318. doi: 10.1136/ard.32.4.316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Read W. K., Bay W. W. Basic cellular lesion in chloroquine toxicity. Lab Invest. 1971 Mar;24(3):246–259. [PubMed] [Google Scholar]
  10. Wibo M., Poole B. Protein degradation in cultured cells. II. The uptake of chloroquine by rat fibroblasts and the inhibition of cellular protein degradation and cathepsin B1. J Cell Biol. 1974 Nov;63(2 Pt 1):430–440. doi: 10.1083/jcb.63.2.430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Zvaifler N. J. Antimalarial treatment of rheumatoid arthritis. Med Clin North Am. 1968 May;52(3):759–764. [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES