Skip to main content
PMC home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1985 May;44(5):316–320. doi: 10.1136/ard.44.5.316

Prostaglandin (PG) E2 generation by cultured canine synovial fibroblasts exposed to microcrystals containing calcium.

D J McCarty, H S Cheung
PMCID: PMC1001639  PMID: 2988464

Abstract

Immunoreactive prostaglandin (PG) E2 was released into the ambient medium in a dose dependent fashion when either hydroxyapatite (HA) or calcium pyrophosphate dihydrate (CPPD) crystals were added to canine synovial fibroblasts in tissue culture. PGE2 release peaked 6 to 9 hours after HA or CPPD crystals were added in the presence of serum but at 24 hours if they were added in the presence of lactalbumin hydrolysate. PGE2 release correlated with crystal endocytosis estimated qualitatively by serial phase contrast microscopy and time lapse photography. As postulated previously by others for monosodium urate crystals, prostaglandin production by synovial cells may also be related to the pathogenesis of the destructive arthropathies associated with HA or CPPD crystals.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cheung H. S., Halverson P. B., McCarty D. J. Phagocytosis of hydroxyapatite or calcium pyrophosphate dihydrate crystals by rabbit articular chondrocytes stimulates release of collagenase, neutral protease, and prostaglandins E2 and F2 alpha. Proc Soc Exp Biol Med. 1983 Jun;173(2):181–189. doi: 10.3181/00379727-173-41628. [DOI] [PubMed] [Google Scholar]
  2. Cheung H. S., Story M. T., McCarty D. J. Mitogenic effects of hydroxyapatite and calcium pyrophosphate dihydrate crystals on cultured mammalian cells. Arthritis Rheum. 1984 Jun;27(6):668–674. doi: 10.1002/art.1780270610. [DOI] [PubMed] [Google Scholar]
  3. Clarris B. J., Fraser J. R., Moran C. J., Muirden K. D. Rheumatoid synovial cells from intact joints. Morphology, growth, and polykaryocytosis. Ann Rheum Dis. 1977 Aug;36(4):293–301. doi: 10.1136/ard.36.4.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hasselbacher P. Stimulation of synovial fibroblasts by calcium oxalate and monosodium urate monohydrate. A mechanism of connective tissue degradation in oxalosis and gout. J Lab Clin Med. 1982 Dec;100(6):977–985. [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Mandel N. S. Structural changes in sodium urate crystals on heating. Arthritis Rheum. 1980 Jun;23(6):772–776. doi: 10.1002/art.1780230610. [DOI] [PubMed] [Google Scholar]
  7. McCarty D. J., Halverson P. B., Carrera G. F., Brewer B. J., Kozin F. "Milwaukee shoulder"--association of microspheroids containing hydroxyapatite crystals, active collagenase, and neutral protease with rotator cuff defects. I. Clinical aspects. Arthritis Rheum. 1981 Mar;24(3):464–473. doi: 10.1002/art.1780240303. [DOI] [PubMed] [Google Scholar]
  8. McCarty D. J., Jr, Gatter R. A. Recurrent acute inflammation associated with focal apatite crystal deposition. Arthritis Rheum. 1966 Dec;9(6):804–819. doi: 10.1002/art.1780090608. [DOI] [PubMed] [Google Scholar]
  9. McCarty D. J., Palmer D. W., James C. CLearance of calcium pyrophosphate dihydrate crystals in vivo. II. Studies using triclinic crystals doubly labeled with 45Ca and 85Sr. Arthritis Rheum. 1979 Oct;22(10):1122–1131. doi: 10.1002/art.1780221012. [DOI] [PubMed] [Google Scholar]
  10. McMillan R. M., Hasselbacher P., Hahn J. L., Harris E. D., Jr Interactions of murine macrophages with monosodium urate crystals: stimulation of lysosomal enzyme release and prostaglandin synthesis. J Rheumatol. 1981 Jul-Aug;8(4):555–562. [PubMed] [Google Scholar]
  11. Menkes C. J., Simon F., Delrieu F., Forest M., Delbarre F. Destructive arthropathy in chondrocalcinosis articularis. Arthritis Rheum. 1976 May-Jun;19 (Suppl 3):329–348. doi: 10.1002/1529-0131(197605/06)19:3+<329::aid-art1780190706>3.0.co;2-z. [DOI] [PubMed] [Google Scholar]
  12. Moncada S., Vane J. R. Pharmacology and endogenous roles of prostaglandin endoperoxides, thromboxane A2, and prostacyclin. Pharmacol Rev. 1978 Sep;30(3):293–331. [PubMed] [Google Scholar]
  13. Robinson D. R., Tashjian A. H., Jr, Levine L. Prostaglandin-stimulated bone resorption by rheumatoid synovia. A possible mechanism for bone destruction in rheumatoid arthritis. J Clin Invest. 1975 Nov;56(5):1181–1188. doi: 10.1172/JCI108195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schumacher H. R., Smolyo A. P., Tse R. L., Maurer K. Arthritis associated with apatite crystals. Ann Intern Med. 1977 Oct;87(4):411–416. doi: 10.7326/0003-4819-87-4-411. [DOI] [PubMed] [Google Scholar]
  15. Silcox D. C., McCarty D. J., Jr Elevated inorganic pyrophosphate concentrations in synovial fluids in osteoarthritis and pseudogout. J Lab Clin Med. 1974 Apr;83(4):518–531. [PubMed] [Google Scholar]
  16. Werb Z., Reynolds J. J. Stimulation by endocytosis of the secretion of collagenase and neutral proteinase from rabbit synovial fibroblasts. J Exp Med. 1974 Dec 1;140(6):1482–1497. doi: 10.1084/jem.140.6.1482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wigley F. M., Fine I. T., Newcombe D. S. The role of the human synovial fibroblast in monosodium urate crystal-induced synovitis. J Rheumatol. 1983 Aug;10(4):602–611. [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES