Skip to main content
NCBI home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1985 Jul;44(7):477–484. doi: 10.1136/ard.44.7.477

Carrageenin-induced arthritis. VI. Alterations in amino acid transport by articular cartilage in acute inflammatory arthritis.

S Byers, C J Handley, D A Lowther, A Sriratana
PMCID: PMC1001679  PMID: 4026408

Abstract

The mechanism of transport of alanine and aminoisobutyric acid into chondrocytes in rabbit articular cartilage was shown to be mediated by transport systems similar to that described for other eukaryotic cells namely the A, ASC, and L systems. Three days after the initiation of an acute inflammatory arthritis by the intra-articular injection of carrageenin into one knee joint the rate of transport of both these amino acids was decreased. Although all three transport systems were depressed, it appeared that the A and ASC systems were partially susceptible to damage by the induced inflammation. The rate of amino acid transport by the affected cartilage had recovered by 28 days after carrageenin treatment. This depression in amino acid transport is discussed in relation to a decrease in general metabolic processes in chondrocytes as a consequence of inflammation.

Full text

PDF
477

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartholomew J. S., Lowther D. A., Handley C. J. Changes in proteoglycan biosynthesis following leukocyte elastase treatment of bovine articular cartilage in culture. Arthritis Rheum. 1984 Aug;27(8):905–912. doi: 10.1002/art.1780270810. [DOI] [PubMed] [Google Scholar]
  2. Bass R., Hedegaard H. B., Dillehay L., Moffett J., Englesberg E. The A, ASC, and L systems for the transport of amino acids in Chinese hamster ovary cells (CHO-K1). J Biol Chem. 1981 Oct 25;256(20):10259–10266. [PubMed] [Google Scholar]
  3. Byers S., Handley C. J., Lowther D. A. Transport of neutral amino acids by adult articular cartilage. Connect Tissue Res. 1983;11(4):321–330. doi: 10.3109/03008208309004864. [DOI] [PubMed] [Google Scholar]
  4. Carmichael D. J., Gillard G. C., Lowther D. A., Handley C. J., Santer V. B. Carrageenin-induced arthritis. IV. Rate changes in cartilage matrix proteoglycan synthesis. Arthritis Rheum. 1977 Apr;20(3):834–840. doi: 10.1002/art.1780200313. [DOI] [PubMed] [Google Scholar]
  5. Catanzaro P. J., Schwartz H. J., Graham R. C., Jr Spectrum and possible mechanism of carrageenan cytotoxicity. Am J Pathol. 1971 Aug;64(2):387–404. [PMC free article] [PubMed] [Google Scholar]
  6. Christensen H. N., Liang M., Archer E. G. A distinct Na+-requiring transport system for alanine, serine, cysteine, and similar amino acids. J Biol Chem. 1967 Nov 25;242(22):5237–5246. [PubMed] [Google Scholar]
  7. Christensen H. N., Oxender D. L., Liang M., Vatz K. A. The use of N-methylation to direct route of mediated transport of amino acids. J Biol Chem. 1965 Sep;240(9):3609–3616. [PubMed] [Google Scholar]
  8. Di Rosa M. Biological properties of carrageenan. J Pharm Pharmacol. 1972 Feb;24(2):89–102. doi: 10.1111/j.2042-7158.1972.tb08940.x. [DOI] [PubMed] [Google Scholar]
  9. Edmondson J. W., Lumeng L., Li T. K. Direct measurement of active transport systems for alanine in freshly isolated rat liver cells. Biochem Biophys Res Commun. 1977 Jun 6;76(3):751–757. doi: 10.1016/0006-291x(77)91564-9. [DOI] [PubMed] [Google Scholar]
  10. Gazzola G. C., Dall'Asta V., Guidotti G. G. The transport of neutral amino acids in cultured human fibroblasts. J Biol Chem. 1980 Feb 10;255(3):929–936. [PubMed] [Google Scholar]
  11. Gazzola G. C., Franchi R., Saibene V., Ronchi P., Guidotti G. G. Regulation of amino acid transport in chick embryo heart cells. I. Adaptive system of mediation for neutral amino acids. Biochim Biophys Acta. 1972 May 9;266(2):407–421. doi: 10.1016/0005-2736(72)90097-1. [DOI] [PubMed] [Google Scholar]
  12. Gillard G. C., Lowther D. A. Carrageenin-induced arthritis. II. Effect of intraarticular injection of carrageenin on the synthesis of proteoglycan in articular cartilage. Arthritis Rheum. 1976 Sep-Oct;19(5):918–922. doi: 10.1002/art.1780190513. [DOI] [PubMed] [Google Scholar]
  13. Handley C. J., Lowther D. A. Extracellular matrix metabolism by chondrocytes. III. Modulation of proteoglycan synthesis by extracellular levels of proteoglycan in cartilage cells in culture. Biochim Biophys Acta. 1977 Nov 7;500(1):132–139. doi: 10.1016/0304-4165(77)90053-8. [DOI] [PubMed] [Google Scholar]
  14. Herman J. H., Nutman T. B., Nozoe M., Mowery C. S., Dennis M. V. Lymphokine-mediated suppression of chondrocyte glycosaminoglycan and protein synthesis. Arthritis Rheum. 1981 Jun;24(6):824–834. doi: 10.1002/art.1780240610. [DOI] [PubMed] [Google Scholar]
  15. Le Cam A., Freychet P. Neutral amino acid transport. Characterization of the A and L systems in isolated rat hepatocytes. J Biol Chem. 1977 Jan 10;252(1):148–156. [PubMed] [Google Scholar]
  16. Lippiello L., Yamamoto K., Robinson D., Mankin H. J. Involvement of prostaglandins from rheumatoid synovium in inhibition of articular cartilage metabolism. Arthritis Rheum. 1978 Nov-Dec;21(8):909–917. doi: 10.1002/art.1780210807. [DOI] [PubMed] [Google Scholar]
  17. Lowther D. A., Gillard G. C., Baxter E., Handley C. J., Rich K. A. Carrageenin-induced arthritis. III. Proteolytic enzymes present in rabbit knee joints after a single intraarticular injection of carrageenin. Arthritis Rheum. 1976 Nov-Dec;19(6):1287–1294. doi: 10.1002/art.1780190609. [DOI] [PubMed] [Google Scholar]
  18. Lowther D. A., Gillard G. C. Carrageenin-induced arthritis. I. The effect of intraarticular carrageenin on the chemical composition of articular cartilage. Arthritis Rheum. 1976 Jul-Aug;19(4):769–776. doi: 10.1002/1529-0131(197607/08)19:4<769::aid-art1780190419>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]
  19. Lowther D. A., Sandy J. D., Santer V. B., Brown H. L. Antigen-induced arthritis. Decreased proteoglycan content and inhibition of proteoglycan synthesis in articular cartilage. Arthritis Rheum. 1978 Jul-Aug;21(6):675–680. doi: 10.1002/art.1780210611. [DOI] [PubMed] [Google Scholar]
  20. OXENDER D. L., CHRISTENSEN H. N. DISTINCT MEDIATING SYSTEMS FOR THE TRANSPORT OF NEUTRAL AMINO ACIDS BY THE EHRLICH CELL. J Biol Chem. 1963 Nov;238:3686–3699. [PubMed] [Google Scholar]
  21. Sandy J. D., Sriratana A., Brown H. L., Lowther D. A. Evidence for polymorphonuclear-leucocyte-derived proteinases in arthritic cartilage. Biochem J. 1981 Jan 1;193(1):193–202. doi: 10.1042/bj1930193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Santer V., Sriratana A., Lowther D. A. Carrageenin-induced arthritis: V. A morphologic study of the development of inflammation in acute arthritis. Semin Arthritis Rheum. 1983 Nov;13(2):160–168. doi: 10.1016/0049-0172(83)90002-1. [DOI] [PubMed] [Google Scholar]
  23. Stevens R. L., Nissley S. P., Kimura J. H., Rechler M. M., Caplan A. I., Hascall V. C. Effects of insulin and multiplication-stimulating activity on proteoglycan biosynthesis in chondrocytes from the Swarm rat chondrosarcoma. J Biol Chem. 1981 Feb 25;256(4):2045–2052. [PubMed] [Google Scholar]
  24. Tager H. S., Christensen H. N. Transport of the four isomers of 2-aminonorbornane-2-carboxylic acid in selected mammalian systems and in Escherichia coli. J Biol Chem. 1971 Dec 25;246(24):7572–7580. [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES