Skip to main content
NCBI home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1985 Oct;44(10):647–657. doi: 10.1136/ard.44.10.647

Hyaluronic acid production in vitro by synovial lining cells from normal and rheumatoid joints.

I M Dahl, G Husby
PMCID: PMC1001731  PMID: 4051585

Abstract

Organ cultures and primary cell cultures were established from synovial tissue collected from patients with rheumatoid arthritis. Hyaluronic acid measured by the incorporation of [3H]glucosamine into the polysaccharide was found to be synthesised in the cultures immediately after transfer from in-vivo to in-vitro conditions. This was in contrast to the primary cultures established from cells isolated from normal joints. The latter cells did not synthesise any detectable hyaluronate. 90-100% of the cells in primary culture were found to be esterase positive, indicating their macrophage nature. The molecular weight of the hyaluronate produced by the pathological cells was low (approximately 50 000) compared with the molecular weight of hyaluronate found in joint fluid from normal or rheumatoid joints. Cell lines of fibroblasts established from rheumatoid joints and studied after four or seven passages also produced hyaluronate of low molecular weight. It is known that similar cell lines from normal joints produce a high molecular weight polymer.

Full text

PDF
647

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abrahamsen T. G., Johnson P. M., Natvig J. B. Membrane characteristics of adherent cells dissociated from rheumatoid synovial tissue. Clin Exp Immunol. 1977 Jun;28(3):474–483. [PMC free article] [PubMed] [Google Scholar]
  2. Anastassiades T. P., Ley J., Wood A. Glycosaminoglycan synthesis and glucose uptake by rheumatoid and nonrheumatoid fibroblastic cells in culture. The effect of nutritional factors. Arthritis Rheum. 1979 Aug;22(8):871–876. doi: 10.1002/art.1780220810. [DOI] [PubMed] [Google Scholar]
  3. BARLAND P., NOVIKOFF A. B., HAMERMAN D. Electron microscopy of the human synovial membrane. J Cell Biol. 1962 Aug;14:207–220. doi: 10.1083/jcb.14.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baker D. G., Dayer J. M., Roelke M., Schumacher H. R., Krane S. M. Rheumatoid synovial cell morphologic changes induced by a mononuclear cell factor in culture. Arthritis Rheum. 1983 Jan;26(1):8–14. doi: 10.1002/art.1780260102. [DOI] [PubMed] [Google Scholar]
  5. Castor C. W., Dorstewitz E. L., Rowe K., Ritchie J. C. Abnormalities of connective tissue cells cultured from patients with rheumatoid arthritis. II. Defective regulation of hyaluronate and collagen formation. J Lab Clin Med. 1971 Jan;77(1):65–75. [PubMed] [Google Scholar]
  6. Clarris B. J., Baxter E., Michelangeli V. P. Activation of human synovial cells by cholera enterotoxin: correlation of morphological responses with adenylate cyclase activities, and the reversing effects of hyaluronidase. Connect Tissue Res. 1982;10(2):173–186. doi: 10.3109/03008208209034417. [DOI] [PubMed] [Google Scholar]
  7. Dahl I. M., Cöster L. Proteoglycan biosynthesis in cultures of corneas and corneal stroma cells from adult rabbits. Exp Eye Res. 1978 Aug;27(2):175–190. doi: 10.1016/0014-4835(78)90087-8. [DOI] [PubMed] [Google Scholar]
  8. Dahl I. M., Johnsen W., Anseth A., Prydz H. The synthesis of glycosaminoglycans by corneal stroma cells in culture. Exp Cell Res. 1974 Sep;88(1):193–197. doi: 10.1016/0014-4827(74)90634-x. [DOI] [PubMed] [Google Scholar]
  9. Dayer J. M., Krane S. M., Russell R. G., Robinson D. R. Production of collagenase and prostaglandins by isolated adherent rheumatoid synovial cells. Proc Natl Acad Sci U S A. 1976 Mar;73(3):945–949. doi: 10.1073/pnas.73.3.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Engström-Laurent A., Hällgren R. Circulating hyaluronate in rheumatoid arthritis: relationship to inflammatory activity and the effect of corticosteroid therapy. Ann Rheum Dis. 1985 Feb;44(2):83–88. doi: 10.1136/ard.44.2.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fantone J. C., Ward P. A. Role of oxygen-derived free radicals and metabolites in leukocyte-dependent inflammatory reactions. Am J Pathol. 1982 Jun;107(3):395–418. [PMC free article] [PubMed] [Google Scholar]
  12. Fraser J. R., Clarris B. J., Baxter E. Patterns of induced variation in the morphology, hyaluronic acid secretion, and lysosomal enzyme activity of cultured human synovial cells. Ann Rheum Dis. 1979 Jun;38(3):287–294. doi: 10.1136/ard.38.3.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fraser J. R., McCall J. F. Culture of synovial cells in vitro. Notes on isolation and propagation. Ann Rheum Dis. 1965 Jul;24(4):351–359. doi: 10.1136/ard.24.4.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hamerman D., Smith C., Keiser H. D., Craig R. Glycosaminoglycans produced by human synovial cell cultures. Coll Relat Res. 1982 Jul;2(4):313–329. doi: 10.1016/s0174-173x(82)80023-x. [DOI] [PubMed] [Google Scholar]
  15. Ikegami S., Taguchi T., Ohashi M., Oguro M., Nagano H., Mano Y. Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-alpha. Nature. 1978 Oct 5;275(5679):458–460. doi: 10.1038/275458a0. [DOI] [PubMed] [Google Scholar]
  16. Janossy G., Panayi G., Duke O., Bofill M., Poulter L. W., Goldstein G. Rheumatoid arthritis: a disease of T-lymphocyte/macrophage immunoregulation. Lancet. 1981 Oct 17;2(8251):839–842. doi: 10.1016/s0140-6736(81)91107-7. [DOI] [PubMed] [Google Scholar]
  17. Klareskog L., Forsum U., Kabelitz D., Plöen L., Sundström C., Nilsson K., Wigren A., Wigzell H. Immune functions of human synovial cells. Phenotypic and T cell regulatory properties of macrophage-like cells that express HLA-DR. Arthritis Rheum. 1982 May;25(5):488–501. doi: 10.1002/art.1780250502. [DOI] [PubMed] [Google Scholar]
  18. Klareskog L., Forsum U., Malmnäs Tjernlund U. K., Kabelitz D., Wigren A. Appearance of anti-HLA-DR-reactive cells in normal and rheumatoid synovial tissue. Scand J Immunol. 1981 Aug;14(2):183–192. doi: 10.1111/j.1365-3083.1981.tb00198.x. [DOI] [PubMed] [Google Scholar]
  19. Klareskog L., Forsum U., Wigzell H. Murine synovial intima contains I-A-, I-E/C-positive bone-marrow-derived cells. Scand J Immunol. 1981 May;15(5):509–514. doi: 10.1111/j.1365-3083.1982.tb00677.x. [DOI] [PubMed] [Google Scholar]
  20. Laurent U. B., Granath K. A. The molecular weight of hyaluronate in the aqueous humour and vitreous body of rabbit and cattle eyes. Exp Eye Res. 1983 Apr;36(4):481–492. doi: 10.1016/0014-4835(83)90042-8. [DOI] [PubMed] [Google Scholar]
  21. Laurent U. B., Tengblad A. Determination of hyaluronate in biological samples by a specific radioassay technique. Anal Biochem. 1980 Dec;109(2):386–394. doi: 10.1016/0003-2697(80)90665-x. [DOI] [PubMed] [Google Scholar]
  22. Mohr W., Beneke G. Synovialzellkulturen für die Erforschung der Pathogenese der Rheumatoiden Arthritis. Beitr Pathol. 1974;153(3):225–243. [PubMed] [Google Scholar]
  23. Myers S. L., Christine T. A. Hyaluronate synthesis by synovial villi in organ culture. Arthritis Rheum. 1983 Jun;26(6):764–770. doi: 10.1002/art.1780260610. [DOI] [PubMed] [Google Scholar]
  24. ROPES M. W., BENNETT G. A., COBB S., JACOX R., JESSAR R. A. 1958 Revision of diagnostic criteria for rheumatoid arthritis. Bull Rheum Dis. 1958 Dec;9(4):175–176. [PubMed] [Google Scholar]
  25. Rubin K., Johansson S., Pettersson I., Ocklind C., Obrink B., Hök M. Attachment of rat hepatocytes to collagen and fibronectin; a study using antibodies directed against cell surface components. Biochem Biophys Res Commun. 1979 Nov 14;91(1):86–94. doi: 10.1016/0006-291x(79)90586-2. [DOI] [PubMed] [Google Scholar]
  26. Rubin K., Oldberg A., Hök M., Obrink B. Adhesion of rat hepatocytes to collagen. Exp Cell Res. 1978 Nov;117(1):165–177. doi: 10.1016/0014-4827(78)90439-1. [DOI] [PubMed] [Google Scholar]
  27. Saarni H., Tammi M., Vuorio E., Penttinen R. Distribution of glycosaminoglycans in rheumatoid cultures and effects of cortisol on it. Scand J Rheumatol. 1980;9(1):11–16. [PubMed] [Google Scholar]
  28. Vuorio E., Einola S., Hakkarainen S., Penttinen R. Synthesis of underpolymerized hyaluronic acid by fibroblasts cultured from rheumatoid and non-rheumatoid synovitis. Rheumatol Int. 1982;2(3):97–102. doi: 10.1007/BF00541160. [DOI] [PubMed] [Google Scholar]
  29. Vuorio E., Takala I., Pulkki K., Einola S. Effects of sodium aurothiomalate on hyaluronic acid synthesis in normal and rheumatoid synovial fibroblast cultures. Scand J Rheumatol. 1979;8(3):173–176. doi: 10.3109/03009747909114451. [DOI] [PubMed] [Google Scholar]
  30. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]
  31. Yaron M., Yaron I., Smetana O., Eylan E., Herzberg M. Hyaluronic acid produced by human synovial fibroblasts. Effect of polyinosinic-polycytidylic acid (poly I:C) and interferon. Arthritis Rheum. 1976 Nov-Dec;19(6):1315–1320. doi: 10.1002/art.1780190612. [DOI] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES