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. 1985 Nov;44(11):742–746. doi: 10.1136/ard.44.11.742

A seroepidemiological study of cytomegalovirus and Epstein-Barr virus in rheumatoid arthritis and sicca syndrome.

P J Venables, M G Ross, P J Charles, R D Melsom, P D Griffiths, R N Maini
PMCID: PMC1001762  PMID: 2998290

Abstract

Antibodies to cytomegalovirus (CMV) and Epstein-Barr virus capsid antigen (EBVCA) were examined in 41 patients with rheumatoid arthritis (RA), 26 patients with primary sicca syndrome, and 26 healthy subjects of similar age and sex. IgG antibody titres to EBVCA and CMV were similar in all three groups, apart from a trivial increase of antibodies to EBVCA in RA. False positive IgM anti-CMV antibodies detected in serum from one patient with sicca syndrome and 20 patients with RA were shown to be due to rheumatoid factors. These data did not support recent suggestions that patients with these diseases showed exaggerated immunological responses to either virus and emphasised the need to incorporate adequate laboratory and disease controls when seroepidemiological studies are performed on sera containing rheumatoid factors and autoantibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akizuki M., Powers R., Holman H. R. A soluble acidic protein of the cell nucleus which reacts with serum from patients with systemic lupus erythermatosus and Sjögren's syndrome. J Clin Invest. 1977 Feb;59(2):264–272. doi: 10.1172/JCI108637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alspaugh M. A., Henle G., Lennette E. T., Henle W. Elevated levels of antibodies to Epstein-Barr virus antigens in sera and synovial fluids of patients with rheumatoid arthritis. J Clin Invest. 1981 Apr;67(4):1134–1140. doi: 10.1172/JCI110127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alspaugh M. A., Jensen F. C., Rabin H., Tan E. M. Lymphocytes transformed by Epstein-Barr virus. Induction of nuclear antigen reactive with antibody in rheumatoid arthritis. J Exp Med. 1978 Apr 1;147(4):1018–1027. doi: 10.1084/jem.147.4.1018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Alspaugh M. A., Talal N., Tan E. M. Differentiation and characterization of autoantibodies and their antigens in Sjögren's syndrome. Arthritis Rheum. 1976 Mar-Apr;19(2):216–222. doi: 10.1002/art.1780190214. [DOI] [PubMed] [Google Scholar]
  5. Aslpaugh M. A., Tan E. M. Serum antibody in rheumatoid arthritis reactive with a cell-associated antigen. Demonstration by precipitation and immunofluorescence. Arthritis Rheum. 1976 Jul-Aug;19(4):711–719. doi: 10.1002/1529-0131(197607/08)19:4<711::aid-art1780190409>3.0.co;2-i. [DOI] [PubMed] [Google Scholar]
  6. Billings P. B., Hoch S. O., White P. J., Carson D. A., Vaughan J. H. Antibodies to the Epstein-Barr virus nuclear antigen and to rheumatoid arthritis nuclear antigen identify the same polypeptide. Proc Natl Acad Sci U S A. 1983 Dec;80(23):7104–7108. doi: 10.1073/pnas.80.23.7104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Booth J. C., Hannington G., Bakir T. M., Stern H., Kangro H., Griffiths P. D., Heath R. B. Comparison of enzyme-linked immunosorbent assay, radioimmunoassay, complement fixation, anticomplement immunofluorescence and passive haemagglutination techniques for detecting cytomegalovirus IgG antibody. J Clin Pathol. 1982 Dec;35(12):1345–1348. doi: 10.1136/jcp.35.12.1345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Douzou P., Keh E., Balny C. Cryoenzymology in aqueous media: Micellar solubilized water clusters. Proc Natl Acad Sci U S A. 1979 Feb;76(2):681–684. doi: 10.1073/pnas.76.2.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ferrell P. B., Aitcheson C. T., Pearson G. R., Tan E. M. Seroepidemiological study of relationships between Epstein-Barr virus and rheumatoid arthritis. J Clin Invest. 1981 Mar;67(3):681–687. doi: 10.1172/JCI110083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Francoeur A. M., Mathews M. B. Interaction between VA RNA and the lupus antigen La: formation of a ribonucleoprotein particle in vitro. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6772–6776. doi: 10.1073/pnas.79.22.6772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Griffiths P. D., Kangro H. O. A user's guide to the indirect solid-phase radioimmunoassay for the detection of cytomegalovirus-specific IgM antibodies. J Virol Methods. 1984 Jul;8(4):271–282. doi: 10.1016/0166-0934(84)90064-8. [DOI] [PubMed] [Google Scholar]
  12. Griffiths P. D., Stagno S., Pass R. F., Smith R. J., Alford C. A., Jr Congenital cytomegalovirus infection: diagnostic and prognostic significance of the detection of specific immunoglobulin M antibodies in cord serum. Pediatrics. 1982 May;69(5):544–549. [PubMed] [Google Scholar]
  13. Hennessy K., Heller M., van Santen V., Kieff E. Simple repeat array in Epstein-Barr virus DNA encodes part of the Epstein-Barr nuclear antigen. Science. 1983 Jun 24;220(4604):1396–1398. doi: 10.1126/science.6304878. [DOI] [PubMed] [Google Scholar]
  14. Kurilla M. G., Keene J. D. The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodies. Cell. 1983 Oct;34(3):837–845. doi: 10.1016/0092-8674(83)90541-x. [DOI] [PubMed] [Google Scholar]
  15. Lerner M. R., Andrews N. C., Miller G., Steitz J. A. Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1981 Feb;78(2):805–809. doi: 10.1073/pnas.78.2.805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Luka J., Kreofsky T., Pearson G. R., Hennessy K., Kieff E. Identification and characterization of a cellular protein that cross-reacts with the Epstein-Barr virus nuclear antigen. J Virol. 1984 Dec;52(3):833–838. doi: 10.1128/jvi.52.3.833-838.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mattioli M., Reichlin M. Heterogeneity of RNA protein antigens reactive with sera of patients with systemic lupus erythematosus. Description of a cytoplasmic nonribosomal antigen. Arthritis Rheum. 1974 Jul-Aug;17(4):421–429. doi: 10.1002/art.1780170413. [DOI] [PubMed] [Google Scholar]
  18. ROPES M. W., BENNETT G. A., COBB S., JACOX R., JESSAR R. A. 1958 Revision of diagnostic criteria for rheumatoid arthritis. Bull Rheum Dis. 1958 Dec;9(4):175–176. [PubMed] [Google Scholar]
  19. Rheumatoid arthritis nuclear antigen and Epstein-Barr virus. Arthritis Rheum. 1984 Apr;27(4):476–478. doi: 10.1002/art.1780270419. [DOI] [PubMed] [Google Scholar]
  20. Shillitoe E. J., Daniels T. E., Whitcher J. P., Vibeke Strand C., Talal N., Greenspan J. S. Antibody to cytomegalovirus in patients with Sjögren's syndrome. As determined by an enzyme-linked immunosorbent assay. Arthritis Rheum. 1982 Mar;25(3):260–265. doi: 10.1002/art.1780250303. [DOI] [PubMed] [Google Scholar]
  21. Smith P. R., Williams D. G., Venables P. J., Maini R. N. Monoclonal antibodies to the Sjögren's syndrome associated antigen SS-B (La). J Immunol Methods. 1985 Feb 28;77(1):63–76. doi: 10.1016/0022-1759(85)90184-x. [DOI] [PubMed] [Google Scholar]
  22. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]
  23. Venables P. J., Charles P. J., Buchanan R. R., Yi T., Mumford P. A., Schrieber L., Room G. R., Maini R. N. Quantitation and detection of isotypes of anti-SS-B antibodies by ELISA and Farr assays using affinity purified antigens. An approach to the investigation of Sjögren's syndrome and systemic lupus erythematosus. Arthritis Rheum. 1983 Feb;26(2):146–155. doi: 10.1002/art.1780260205. [DOI] [PubMed] [Google Scholar]
  24. Venables P. J., Roffe L. M., Erhardt C. C., Maini R. N., Edwards J. M., Porter A. D. Titers of antibodies to RANA in rheumatoid arthritis and normal sera. Relationship to Epstein-Barr virus infection. Arthritis Rheum. 1981 Dec;24(12):1459–1468. doi: 10.1002/art.1780241201. [DOI] [PubMed] [Google Scholar]

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