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. 1986 May;45(5):353–358. doi: 10.1136/ard.45.5.353

The SL autoantibody-antigen system: clinical and biochemical studies.

R M Bernstein, S H Morgan, C C Bunn, R C Gainey, G R Hughes, M B Mathews
PMCID: PMC1001891  PMID: 3487291

Abstract

A recently described autoantibody, SL, was found in serum from 27 patients with autoimmune disease, including 20 with systemic lupus erythematosus (SLE) where the frequently was 7%. Analysis of clinical, serological, and HLA data from 119 SLE patients showed no positive associations with anti-SL antibody apart from a higher frequency of non-infective fever. Most SL positive sera contained other precipitins, notably antibodies to Ro(SS-A) and the proliferating cell nuclear antigen, PCNA. Anti-SL IgG recognised a protein of 32 000 daltons without associated RNA. This polypeptide was distinguished from a similarly sized component of the Sm and RNP ribonucleoprotein particles by demonstrating different products of partial proteolysis. Although anti-SL antibody is of limited clinical importance, it occurs with twice the frequency of anti-SM antibody in white patients with SLE. Preliminary studies indicate that SL and the Japanese Ki system are identical.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alarcon-Segovia D. Mixed connective tissue disease - a decade of growing pains. J Rheumatol. 1981 Jul-Aug;8(4):535–540. [PubMed] [Google Scholar]
  2. Bernstein R. M., Bunn C. C., Hughes G. R., Francoeur A. M., Mathews M. B. Cellular protein and RNA antigens in autoimmune disease. Mol Biol Med. 1984 Apr;2(2):105–120. [PubMed] [Google Scholar]
  3. Bernstein R. M., Bunn C. C., Hughes G. R. Identification of antibodies to acidic antigens by counterimmunoelectrophoresis. Ann Rheum Dis. 1982 Oct;41(5):554–555. doi: 10.1136/ard.41.5.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bernstein R. M., Steigerwald J. C., Tan E. M. Association of antinuclear and antinucleolar antibodies in progressive systemic sclerosis. Clin Exp Immunol. 1982 Apr;48(1):43–51. [PMC free article] [PubMed] [Google Scholar]
  5. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  6. Francoeur A. M., Mathews M. B. Interaction between VA RNA and the lupus antigen La: formation of a ribonucleoprotein particle in vitro. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6772–6776. doi: 10.1073/pnas.79.22.6772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Mathews M. B., Bernstein R. M., Franza B. R., Jr, Garrels J. I. Identity of the proliferating cell nuclear antigen and cyclin. Nature. 1984 May 24;309(5966):374–376. doi: 10.1038/309374a0. [DOI] [PubMed] [Google Scholar]
  9. Mathews M. B., Reichlin M., Hughes G. R., Bernstein R. M. Anti-threonyl-tRNA synthetase, a second myositis-related autoantibody. J Exp Med. 1984 Aug 1;160(2):420–434. doi: 10.1084/jem.160.2.420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Notman D. D., Kurata N., Tan E. M. Profiles of antinuclear antibodies in systemic rheumatic diseases. Ann Intern Med. 1975 Oct;83(4):464–469. doi: 10.7326/0003-4819-83-4-464. [DOI] [PubMed] [Google Scholar]
  11. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]
  12. Tojo T., Kaburaki J., Hayakawa M., Okamoto T., Tomii M., Homma M. Precipitating antibody to a soluble nuclear antigen "Ki" with specificity for systemic lupus erythematosus. Ryumachi. 1981;21 (Suppl):129–140. [PubMed] [Google Scholar]
  13. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Treadwell E. L., Alspaugh M. A., Sharp G. C. Characterization of a new antigen-antibody system (Su) in patients with systemic lupus erythematosus. Arthritis Rheum. 1984 Nov;27(11):1263–1271. doi: 10.1002/art.1780271108. [DOI] [PubMed] [Google Scholar]
  15. Venables P. J., Smith P. R., Maini R. N. Purification and characterization of the Sjögren's syndrome A and B antigens. Clin Exp Immunol. 1983 Dec;54(3):731–738. [PMC free article] [PubMed] [Google Scholar]
  16. Wooley P. H., Griffin J., Panayi G. S., Batchelor J. R., Welsh K. I., Gibson T. J. HLA-DR antigens and toxic reaction to sodium aurothiomalate and D-penicillamine in patients with rheumatoid arthritis. N Engl J Med. 1980 Aug 7;303(6):300–302. doi: 10.1056/NEJM198008073030602. [DOI] [PubMed] [Google Scholar]

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