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. 1986 Nov;45(11):892–898. doi: 10.1136/ard.45.11.892

Analysis of circulating immune complexes from patients with ankylosing spondylitis by gel electrophoresis and immunoblotting using antiserum against a psoriasis associated retrovirus-like particle.

E Rødahl, O J Iversen
PMCID: PMC1002016  PMID: 3539036

Abstract

Circulating immune complexes (CIC) were isolated from patients with ankylosing spondylitis (AS) and healthy blood donors by isopycnic ultracentrifugation in sucrose gradients. The CIC were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The major components of the CIC were identified as albumin, immunoglobulins, and complement factors. A 70 kD component and several low molecular weight components (Mr 19 kD and 14 kD (doublet] were detectable only in CIC from patients with AS. An antiserum raised against the envelope glycoprotein, gp70, of a psoriasis associated retrovirus-like particle was applied to check for cross reacting activity. This antiserum reacted with both a 70 kD and a 40-45 kD component in CIC from three out of six patients but not with CIC from any of the blood donors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crawford T. B., Adams D. S., Cheevers W. P., Cork L. C. Chronic arthritis in goats caused by a retrovirus. Science. 1980 Feb 29;207(4434):997–999. doi: 10.1126/science.6153243. [DOI] [PubMed] [Google Scholar]
  2. Dalen A. B., Hellgren L., Iversen O. J., Vincent J. A virus-like particle associated with psoriasis. Acta Pathol Microbiol Immunol Scand B. 1983 Aug;91(4):221–229. doi: 10.1111/j.1699-0463.1983.tb00037.x. [DOI] [PubMed] [Google Scholar]
  3. Espinoza L. R., Gaylord S. W., Bocanegra T. S., Vasey F. B., Germain B. F. Circulating immune complexes in the seronegative spondyloarthropathies. Clin Immunol Immunopathol. 1982 Mar;22(3):384–393. doi: 10.1016/0090-1229(82)90055-1. [DOI] [PubMed] [Google Scholar]
  4. Gessain A., Barin F., Vernant J. C., Gout O., Maurs L., Calender A., de Thé G. Antibodies to human T-lymphotropic virus type-I in patients with tropical spastic paraparesis. Lancet. 1985 Aug 24;2(8452):407–410. doi: 10.1016/s0140-6736(85)92734-5. [DOI] [PubMed] [Google Scholar]
  5. Hang L., Theofilopoulos A. N., Dixon F. J. A spontaneous rheumatoid arthritis-like disease in MRL/l mice. J Exp Med. 1982 Jun 1;155(6):1690–1701. doi: 10.1084/jem.155.6.1690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Iversen O. J., Dalen A. B. The major internal protein, p27, of a retrovirus-like particle is expressed in blood lymphocytes from psoriatic patients. Arch Virol. 1985;85(3-4):197–207. doi: 10.1007/BF01314231. [DOI] [PubMed] [Google Scholar]
  7. Iversen O. J., Dalen A. B. Urine proteins cross-reacting with antiserum against virus-like particles from cultured psoriatic epithelial cells. Acta Pathol Microbiol Immunol Scand B. 1983 Oct;91(5):343–349. doi: 10.1111/j.1699-0463.1983.tb00058.x. [DOI] [PubMed] [Google Scholar]
  8. Iversen O. J. Isolation of virus-like particles in urine from a psoriatic patient. Acta Pathol Microbiol Immunol Scand B. 1983 Dec;91(6):407–412. doi: 10.1111/j.1699-0463.1983.tb00068.x. [DOI] [PubMed] [Google Scholar]
  9. Iversen O. J., Rødahl E., Dalen A. B. Rabbit antibodies against the major internal protein of a retrovirus-like particle bind to epidermal cells in psoriatic skin. Brief report. Arch Virol. 1985;86(3-4):341–346. doi: 10.1007/BF01309838. [DOI] [PubMed] [Google Scholar]
  10. Iversen O. J., Rødahl E. The major internal protein, p27, of a retrovirus-like particle participates in immune complex formation in psoriasis. Arch Virol. 1985;86(1-2):37–45. doi: 10.1007/BF01314112. [DOI] [PubMed] [Google Scholar]
  11. Izui S., McConahey P. J., Theofilopoulos A. N., Dixon F. J. Association of circulating retroviral gp70-anti-gp70 immune complexes with murine systemic lupus erythematosus. J Exp Med. 1979 May 1;149(5):1099–1116. doi: 10.1084/jem.149.5.1099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Levy J. A., Shimabukuro J., Hollander H., Mills J., Kaminsky L. Isolation of AIDS-associated retroviruses from cerebrospinal fluid and brain of patients with neurological symptoms. Lancet. 1985 Sep 14;2(8455):586–588. [PubMed] [Google Scholar]
  13. Maire M. A., Barnet M., Carpentier N., Miescher P. A., Lambert P. H. Identification of components of IC purified from human sera. I. Immune complexes purified from sera of patients with SLE. Clin Exp Immunol. 1983 Feb;51(2):215–224. [PMC free article] [PubMed] [Google Scholar]
  14. Male D., Roitt I. M., Hay F. C. Analysis of immune complexes in synovial effusions of patients with rheumatoid arthritis. Clin Exp Immunol. 1980 Feb;39(2):297–306. [PMC free article] [PubMed] [Google Scholar]
  15. Markenson J. A., Snyder H. W., Jr Reactivity of antisera to endogenous primate retrovirus with a human T cell membrane protein: recognition of a nonviral glycoprotein by antibodies directed only against carbohydrate components. J Immunol. 1984 Feb;132(2):772–779. [PubMed] [Google Scholar]
  16. McGuire T. C., Crawford T. B., Henson J. B. Equine infectious anemia: detection of infections virus-antibody complexes in the serum. Immunol Commun. 1972;1(6):545–551. doi: 10.3109/08820137209022963. [DOI] [PubMed] [Google Scholar]
  17. Munthe E., Natvig J. B. Complement-fixing intracellular complexes of IgG rheumatoid factor in rheumatoid plasma cells. Scand J Immunol. 1972;1(3):217–229. doi: 10.1111/j.1365-3083.1972.tb01813.x. [DOI] [PubMed] [Google Scholar]
  18. Panem S., Reynolds J. T. Retrovirus expression in normal and pathogenic processes of man. Fed Proc. 1979 Dec;38(13):2674–2678. [PubMed] [Google Scholar]
  19. Pedersen N. C., Pool R. R., O'Brien T. Feline chronic progressive polyarthritis. Am J Vet Res. 1980 Apr;41(4):522–535. [PubMed] [Google Scholar]
  20. Per S. R., Abruzzo J. L., Heimer R. Analysis of immune complexes by two-dimensional gel electrophoresis. Clin Immunol Immunopathol. 1985 Feb;34(2):165–173. doi: 10.1016/0090-1229(85)90021-2. [DOI] [PubMed] [Google Scholar]
  21. Rødahl E. Isopycnic ultracentrifugation of immune complexes. Scand J Immunol. 1985 Jan;21(1):93–98. doi: 10.1111/j.1365-3083.1985.tb01407.x. [DOI] [PubMed] [Google Scholar]
  22. Rødahl E., Iversen O. J. Antigens related to the major internal protein, p27, of a psoriasis associated retrovirus-like particle are expressed in patients with chronic arthritis. Ann Rheum Dis. 1985 Nov;44(11):761–765. doi: 10.1136/ard.44.11.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rødahl E., Iversen O. J., Dalen A. B. Preparative isolation of immune complexes from serum by sucrose gradient ultracentrifugation. Scand J Immunol. 1984 Jul;20(1):21–26. doi: 10.1111/j.1365-3083.1984.tb00973.x. [DOI] [PubMed] [Google Scholar]
  24. Schüpbach J., Kalyanaraman V. S., Sarngadharan M. G., Bunn P. A., Blayney D. W., Gallo R. C. Demonstration of viral antigen p24 in circulating immune complexes of two patients with human T-cell leukaemia/lymphoma virus (HTLV) positive lymphoma. Lancet. 1984 Feb 11;1(8372):302–305. doi: 10.1016/s0140-6736(84)90358-1. [DOI] [PubMed] [Google Scholar]
  25. Sparrow R. L., Purcell D. F., McKenzie I. F. Cross-reactivity of a normal human cell surface antigen with primate retrovirus glycoproteins. Hum Immunol. 1985 Jun;13(2):83–93. doi: 10.1016/0198-8859(85)90015-1. [DOI] [PubMed] [Google Scholar]
  26. Tucker D. F., Begent R. H., Hogg N. M. Characterization of immune complexes in serum by adsorption on staphylococcal protein A: model studies and application to sera of rats bearing a gross virus-induced lymphoma. J Immunol. 1978 Nov;121(5):1644–1651. [PubMed] [Google Scholar]

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