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. 1987 Feb;46(2):110–113. doi: 10.1136/ard.46.2.110

Sensitivity of lymphocytes from patients with systemic lupus erythematosus to the induction of sister chromatid exchanges by alkylating agents and bromodeoxyuridine.

R G Palmer, C A Smith-Burchnell, C J Doré, A M Denman
PMCID: PMC1002075  PMID: 3827332

Abstract

Several authors have suggested that the cells of patients with systemic lupus erythematosus (SLE) have defects of DNA repair. Cells from patients with the classical chromosomal instability syndromes, thought to be due to defects in DNA repair, are hypersensitive to the chromosome damaging effects of some DNA damaging agents, measured as sister chromatid exchange (SCE). We have observed that lymphocytes of patients with SLE are not more sensitive than lymphocytes from healthy individuals to the SCE inducing effects of the DNA damaging agents methyl nitrosourea, methyl methanesulphonate, chlorambucil, and bromodeoxyuridine. These observations do not support the suggestion that cells of patients with SLE have an underlying defect of DNA repair.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe S., Sasaki M. Chromosome aberrations and sister chromatid exchanges in Chinese hamster cells exposed to various chemicals. J Natl Cancer Inst. 1977 Jun;58(6):1635–1641. doi: 10.1093/jnci/58.6.1635. [DOI] [PubMed] [Google Scholar]
  2. BLOOM D. Congenital telangiectatic erythema resembling lupus erythematosus in dwarfs; probably a syndrome entity. AMA Am J Dis Child. 1954 Dec;88(6):754–758. [PubMed] [Google Scholar]
  3. Beighlie D. J., Teplitz R. L. Repair of UV damaged DNA in systemic lupus erythematosus. J Rheumatol. 1975 Jun;2(2):149–160. [PubMed] [Google Scholar]
  4. Brat S. V. Sister chromatid exchange and cell cycle in fibroblasts of Bloom's syndrome. Hum Genet. 1979 Apr 17;48(1):73–79. doi: 10.1007/BF00273277. [DOI] [PubMed] [Google Scholar]
  5. Chaganti R. S., Schonberg S., German J. A manyfold increase in sister chromatid exchanges in Bloom's syndrome lymphocytes. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4508–4512. doi: 10.1073/pnas.71.11.4508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cunlift P. N., Mann J. R., Cameron A. H., Roberts K. D., Ward H. N. Radiosensitivity in ataxia-telangiectasia. Br J Radiol. 1975 May;48(569):374–376. doi: 10.1259/0007-1285-48-569-374. [DOI] [PubMed] [Google Scholar]
  7. Davidson R. L., Kaufman E. R., Dougherty C. P., Ouellette A. M., DiFolco C. M., Latt S. A. Induction of sister chromatid exchanges by BUdR is largely independent of the BUdR content of DNA. Nature. 1980 Mar 6;284(5751):74–76. doi: 10.1038/284074a0. [DOI] [PubMed] [Google Scholar]
  8. Goth-Goldstein R. Repair of DNA damaged by alkylating carcinogens is defective in xeroderma pigmentosum-derived fibroblasts. Nature. 1977 May 5;267(5606):81–82. doi: 10.1038/267081a0. [DOI] [PubMed] [Google Scholar]
  9. Goto K., Maeda S., Kano Y., Sugiyama T. Factors involved in differential Giemsa-staining of sister chromatids. Chromosoma. 1978 May 16;66(4):351–359. doi: 10.1007/BF00328535. [DOI] [PubMed] [Google Scholar]
  10. Harris G., Asbery L., Lawley P. D., Denman A. M., Hylton W. Defective repair of 0(6)-methylguanine in autoimmune diseases. Lancet. 1982 Oct 30;2(8305):952–956. doi: 10.1016/s0140-6736(82)90159-3. [DOI] [PubMed] [Google Scholar]
  11. Miura K., Morimoto K., Koizumi A. Proliferative kinetics and mitomycin C-induced chromosome damage in Fanconi's anemia lymphocytes. Hum Genet. 1983;63(1):19–23. doi: 10.1007/BF00285391. [DOI] [PubMed] [Google Scholar]
  12. Oikawa A., Sakai S., Horaguchi K., Tohda H. Sensitivities of peripheral lymphocytes from healthy humans to induction of sister chromatid exchanges by chemicals. Cancer Res. 1983 Jan;43(1):439–442. [PubMed] [Google Scholar]
  13. Palmer R. G., Doré C. J., Denman A. M. Chlorambucil-induced chromosome damage to human lymphocytes is dose-dependent and cumulative. Lancet. 1984 Feb 4;1(8371):246–249. doi: 10.1016/s0140-6736(84)90125-9. [DOI] [PubMed] [Google Scholar]
  14. Pedersen F. K., Hertz H., Lundsteen C., Platz P., Thomsen M. Indication of primary immune deficiency in Fanconi's anemia. Acta Paediatr Scand. 1977 Nov;66(6):745–751. doi: 10.1111/j.1651-2227.1977.tb07983.x. [DOI] [PubMed] [Google Scholar]
  15. Perry P., Wolff S. New Giemsa method for the differential staining of sister chromatids. Nature. 1974 Sep 13;251(5471):156–158. doi: 10.1038/251156a0. [DOI] [PubMed] [Google Scholar]
  16. Shiraishi Y., Freeman A. I., Sandberg A. A. Increased sister chromatid exchange in bone marrow and blood cells from Bloom's syndrome. Cytogenet Cell Genet. 1976;17(4):162–173. doi: 10.1159/000130710. [DOI] [PubMed] [Google Scholar]
  17. Solomon E., Bobrow M. Sister chromatid exchanges--a sensitive assay of agents damaging human chromosomes. Mutat Res. 1975 Nov;30(2):273–278. [PubMed] [Google Scholar]
  18. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]
  19. Taniguchi N., Mukai M., Nagaoki T., Miyawaki T., Moriya N., Takahashi H., Kondo N. Impaired B-cell differentiation and T-cell regulatory function in four patients with Bloom's syndrome. Clin Immunol Immunopathol. 1982 Feb;22(2):247–258. doi: 10.1016/0090-1229(82)90041-1. [DOI] [PubMed] [Google Scholar]
  20. Taylor A. M., Rosney C. M., Campbell J. B. Unusual sensitivity of ataxia telangiectasia cells to bleomycin. Cancer Res. 1979 Mar;39(3):1046–1050. [PubMed] [Google Scholar]
  21. Tuschl H., Kovac R., Wolf A., Smolen J. S. SCE frequencies in lymphocytes of systemic lupus erythematosus patients. Mutat Res. 1984 Sep;128(2):167–171. doi: 10.1016/0027-5107(84)90104-0. [DOI] [PubMed] [Google Scholar]
  22. Wolff S., Rodin B., Cleaver J. E. Sister chromatid exchanges induced by mutagenic carcinogens in normal and xeroderma pigmentosum cells. Nature. 1977 Jan 27;265(5592):347–349. doi: 10.1038/265347a0. [DOI] [PubMed] [Google Scholar]
  23. Zamansky G. B., Minka D. F., Deal C. L., Hendricks K. The in vitro photosensitivity of systemic lupus erythematosus skin fibroblasts. J Immunol. 1985 Mar;134(3):1571–1576. [PubMed] [Google Scholar]

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