FIGURE 5.

Human apolipoprotein F (ApoF) favors remnant particle clearance by increasing hepatic uptake. (A) Baseline‐corrected plasma triglyceride (TG) after oral olive oil gavage and calculation of area under the curve in 5 h fasted adenovirus‐expressed green fluorescent protein (Ad‐GFP) or adenoviral overexpression of human ApoF (Ad‐hApoF) mice (n = 9–10 per group). (B) Postheparin plasma lipoprotein lipase (LPL) and hepatic lipase (HL) activities in overnight fasted Ad‐GFP or Ad‐hApoF mice (n = 8 per group). (C) [³H] disappearance from plasma and organ uptake after intravenous (i.v.) injection of very low density lipoprotein (VLDL)‐like particles, double‐labeled with glycerol tri[³H]oleate (TO) and [¹⁴C]cholesteryl oleate (CO) (n = 7–8 per group). (D) [¹⁴C] disappearance from plasma and tissue uptake after i.v. injection of VLDL‐like particles, double‐labeled with TO and [¹⁴C]CO (n = 8 per group). AUC, area under the curve; gWAT, gognadal white adipose tissue; HL, hepatic lipase; iBAT, interscapular brown adipose tissue; iWAT, inguinal white adipose tissue; LPL, lipoprotein lipase; sBAT, subscapular brown adipose tissue. *p < 0.05, **p < 0.01, ***p < 0.001 as compared with the control by t test, Mann–Whitney test or two‐way ANOVA as appropriate. Multiple comparisons were assessed by Fisher's least significant difference post hoc test for (A). All data are shown as the means ± SEM.