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. 2022 Dec 23;72(4):520–531. doi: 10.2337/db22-0744

Figure 2.

Figure 2

Effect of HG on mtDNA sensitivity to MNase digestion and occupancy of LncCytB on mtDNA. A: Nucleosome remodeling on mtDNA was assessed by MNase-assisted nucleosomal DNA sequencing, showing the nucleosomal DNA remodeling patterns in the mtDNA. B: Packaging of mtDNA was accessed in the isolated mitochondria by staining with MTR and SYBR Green and flow cytometry under FL1 488-nm and FL3 640-nm wavelength excitation. Raw Flow Cytometry Standard files were analyzed using WinList 3D version 9. C: Log ratio of LncCytB occupancy on mtDNA in NG and HG in the CYTB region of the mtDNA (13,000–16,000 bp), performed by ChIRP using fluorescein-12-dUTP–incorporated LncCytB followed by next-generation sequencing. Arrowheads indicate blunted or missing peaks. Cont, unstained mitochondria.