Skip to main content
PMC home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1988 Sep;47(9):717–724. doi: 10.1136/ard.47.9.717

Disease activity in systemic lupus erythematosus related to a range of antibodies binding DNA and synthetic polynucleotides.

D A Isenberg 1, C Dudeney 1, W Williams 1, A Todd-Pokropek 1, B D Stollar 1
PMCID: PMC1003587  PMID: 3263086

Abstract

Antibodies to dDNA, nDNA, Z-DNA, poly(dT), poly(I), poly(dG.dC), poly(dA.dT), and total IgG and IgM were measured in five serial bleeds from 39 patients with systemic lupus erythematosus (SLE). The main findings were that those patients with renal disease form a distinct subset whose antibody levels correlate well with disease activity; anti-poly(dT) antibodies showed the best overall correlation with disease activity; and discriminant functional analysis demonstrated a major improvement in correlation of disease activity with combinations of antibodies to dDNA/nDNA/Z-DNA/poly(dT) (generally 50% or more were correctly classified) than with dDNA or nDNA alone (generally less than 25% correct). Serum IgG (but not IgM) correlated significantly (p less than 0.01) with six antibodies, suggesting that polyclonal activation plays a part in the development of these antibodies, though antibody cross reactivity is not excluded.

Full text

PDF
717

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alarcón-Segovia D., Fishbein E. Immunochemical characterization of the anti-RNA antibodies found in scleroderma and systemic lupus erythematosus. I. Differences in reactivity with Poly (U) and Poly-(A) Poly (U). J Immunol. 1975 Jul;115(1):28–31. [PubMed] [Google Scholar]
  2. CEPPELLINI R., POLLI E., CELADA F. A DNA-reacting factor in serum of a patient with lupus erythematosus diffusus. Proc Soc Exp Biol Med. 1957 Dec;96(3):572–574. doi: 10.3181/00379727-96-23544. [DOI] [PubMed] [Google Scholar]
  3. Davis P., Percy J. S., Russell A. S. Correlation between levels of DNA antibodies and clinical disease activity in SLE. Ann Rheum Dis. 1977 Apr;36(2):157–159. doi: 10.1136/ard.36.2.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Isenberg D. A., Dudeney C., Williams W., Addison I., Charles S., Clarke J., Todd-Pokropek A. Measurement of anti-DNA antibodies: a reappraisal using five different methods. Ann Rheum Dis. 1987 Jun;46(6):448–456. doi: 10.1136/ard.46.6.448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Isenberg D. A., Shoenfeld Y., Schwartz R. S. Multiple serologic reactions and their relationship to clinical activity in systemic lupus erythematosus. Arthritis Rheum. 1984 Feb;27(2):132–138. doi: 10.1002/art.1780270203. [DOI] [PubMed] [Google Scholar]
  6. Koffler D., Carr R., Agnello V., Thoburn R., Kunkel H. G. Antibodies to polynucleotides in human sera: antigenic specificity and relation to disease. J Exp Med. 1971 Jul 1;134(1):294–312. doi: 10.1084/jem.134.1.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lafer E. M., Rauch J., Andrzejewski C., Jr, Mudd D., Furie B., Furie B., Schwartz R. S., Stollar B. D. Polyspecific monoclonal lupus autoantibodies reactive with both polynucleotides and phospholipids. J Exp Med. 1981 Apr 1;153(4):897–909. doi: 10.1084/jem.153.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lange A. Evaluation of the simultaneous estimation of anti-dsDNA and anti-ssDNA antibodies for clinical purposes. Clin Exp Immunol. 1978 Mar;31(3):472–481. [PMC free article] [PubMed] [Google Scholar]
  9. Ludivico C. L., Zweiman B., Myers A. R., Hebert J., Green P. A. Predictive value of anti-DNA antibody and selected laboratory studies in systemic lupus erythematosus. J Rheumatol. 1980 Nov-Dec;7(6):843–849. [PubMed] [Google Scholar]
  10. MIESCHER P., STRASSLE R. New serological methods for the detection of the L.E. factor. Vox Sang. 1957 Sep;2(4):283–287. doi: 10.1111/j.1423-0410.1957.tb03704.x. [DOI] [PubMed] [Google Scholar]
  11. Miniter M. F., Stollar B. D., Agnello V. Reassessment of the clinical significance of native DNA antibodies in systemic lupus erythematosus. Arthritis Rheum. 1979 Sep;22(9):959–968. doi: 10.1002/art.1780220903. [DOI] [PubMed] [Google Scholar]
  12. Morrow W. J., Isenberg D. A., Todd-Pokropek A., Parry H. F., Snaith M. L. Useful laboratory measurements in the management of systemic lupus erythematosus. Q J Med. 1982 Spring;51(202):125–138. [PubMed] [Google Scholar]
  13. ROBBINS W. C., HOLMAN H. R., DEICHER H., KUNKEL H. G. Complement fixation with cell nuclei and DNA in lupus erythematosus. Proc Soc Exp Biol Med. 1957 Dec;96(3):575–579. doi: 10.3181/00379727-96-23545. [DOI] [PubMed] [Google Scholar]
  14. Rothfield N. F., Stollar B. D. The relation of immunoglobulin class, pattern of anti-nuclear antibody, and complement-fixing antibodies to DNA in sera from patients with systemic lupus erythematosus. J Clin Invest. 1967 Nov;46(11):1785–1794. doi: 10.1172/JCI105669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SELIGMANN M. Mise en évidence dans le sérum de malades atteints de lupus erythémateux disséminé d'une substance déterminant une réaction de précipitation avec l'acide désoxyribonucléique. C R Hebd Seances Acad Sci. 1957 Jul 8;245(2):243–245. [PubMed] [Google Scholar]
  16. Schur P. H., Monroe M. Antibodies to ribonucleic acid in systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1108–1112. doi: 10.1073/pnas.63.4.1108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schur P. H., Sandson J. Immunologic factors and clinical activity in systemic lupus erythematosus. N Engl J Med. 1968 Mar 7;278(10):533–538. doi: 10.1056/NEJM196803072781004. [DOI] [PubMed] [Google Scholar]
  18. Schwartz R. S., Stollar B. D. Origins of anti-DNA autoantibodies. J Clin Invest. 1985 Feb;75(2):321–327. doi: 10.1172/JCI111704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Scott J. S., Maddison P. J., Taylor P. V., Esscher E., Scott O., Skinner R. P. Connective-tissue disease, antibodies to ribonucleoprotein, and congenital heart block. N Engl J Med. 1983 Jul 28;309(4):209–212. doi: 10.1056/NEJM198307283090403. [DOI] [PubMed] [Google Scholar]
  20. Shoenfeld Y., Smorodinsky N. I., Lavie G., Hazaz B., Joshua H., Pinkhas J. Human lupus monoclonal autoantibodies bind to Raji cells. Immunol Lett. 1985;11(2):121–126. doi: 10.1016/0165-2478(85)90154-3. [DOI] [PubMed] [Google Scholar]
  21. Sibley J. T., Lee J. S., Decoteau W. E. Left-handed "Z" DNA antibodies in rheumatoid arthritis and systemic lupus erythematosus. J Rheumatol. 1984 Oct;11(5):633–637. [PubMed] [Google Scholar]
  22. Stollar B. D. Antibodies to DNA. CRC Crit Rev Biochem. 1986;20(1):1–36. doi: 10.3109/10409238609115899. [DOI] [PubMed] [Google Scholar]
  23. Swaak A. J., Aarden L. A., Statius van Eps L. W., Feltkamp T. E. Anti-dsDNA and complement profiles as prognostic guides in systemic lupus erythematosus. Arthritis Rheum. 1979 Mar;22(3):226–235. doi: 10.1002/art.1780220304. [DOI] [PubMed] [Google Scholar]
  24. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]
  25. Tan E. M., Schur P. H., Carr R. I., Kunkel H. G. Deoxybonucleic acid (DNA) and antibodies to DNA in the serum of patients with systemic lupus erythematosus. J Clin Invest. 1966 Nov;45(11):1732–1740. doi: 10.1172/JCI105479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Weinstein A., Bordwell B., Stone B., Tibbetts C., Rothfield N. F. Antibodies to native DNA and serum complement (C3) levels. Application to diagnosis and classification of systemic lupus erythematosus. Am J Med. 1983 Feb;74(2):206–216. doi: 10.1016/0002-9343(83)90613-7. [DOI] [PubMed] [Google Scholar]
  27. Zouali M., Stollar B. D. A rapid ELISA for measurement of antibodies to nucleic acid antigens using UV-treated polystyrene microplates. J Immunol Methods. 1986 Jun 10;90(1):105–110. doi: 10.1016/0022-1759(86)90390-x. [DOI] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES