Skip to main content
NCBI home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1989 Sep;48(9):753–759. doi: 10.1136/ard.48.9.753

Anionic salivary proteins associated with connective tissue disorders: sialated tissue kallikreins.

D Greaves 1, J T Whicher 1, K D Bhoola 1, C Scully 1, S Flint 1, S R Porter 1, R E Chambers 1, P A Maddison 1, J A Beeley 1, R Matthews 1
PMCID: PMC1003869  PMID: 2802797

Abstract

Parotid saliva was collected from 32 patients with rheumatoid arthritis, 10 with systemic lupus erythematosus, three with mixed connective tissue disease, 12 with progressive systemic sclerosis, two with primary Sjögren's syndrome, and four with Raynaud's syndrome. Tissue kallikreins were measured by radioimmunoassay, and saliva samples were subjected to isoelectric focusing followed by immunoblotting or silver staining. The results showed that the saliva of patients with connective tissue diseases contained increased amounts of immunoreactive tissue kallikrein. In addition, there was an increase in the multiple forms of anionic tissue kallikreins, resulting mainly from a shift in their distribution towards that of higher sialic acid content and lower isoelectric point. These changes were most obvious in patients with systemic lupus erythematosus. Novel or unusual glycosylation may explain the occurrence of increased amounts of anionic salivary proteins in connective tissue diseases.

Full text

PDF
753

Images in this article

755Image
on p.755

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benedek-Spät E., Puskás M., Rajki J. Electrophoretic protein pattern of human parotid saliva in Sjögren's syndrome and sialosis. Acta Otolaryngol. 1980 Sep-Oct;90(3-4):275–282. doi: 10.3109/00016488009131726. [DOI] [PubMed] [Google Scholar]
  2. Bhoola K. D., McNicol M. W., Oliver S., Foran J. Changes in salivary enzymes in patients with sarcoidosis. N Engl J Med. 1969 Oct 16;281(16):877–879. doi: 10.1056/NEJM196910162811605. [DOI] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Chisholm D. M., Beeley J. A., Mason D. K. Salivary proteins in Sjögren's syndrome: separation by isoelectric focusing in acrylamide gels. Oral Surg Oral Med Oral Pathol. 1973 May;35(5):620–630. doi: 10.1016/0030-4220(73)90027-3. [DOI] [PubMed] [Google Scholar]
  5. Evans B. A., Yun Z. X., Close J. A., Tregear G. W., Kitamura N., Nakanishi S., Callen D. F., Baker E., Hyland V. J., Sutherland G. R. Structure and chromosomal localization of the human renal kallikrein gene. Biochemistry. 1988 May 3;27(9):3124–3129. doi: 10.1021/bi00409a003. [DOI] [PubMed] [Google Scholar]
  6. Fischer C. J., Wyshak G. H., Weisberger D. Sjögren's syndrome. Electrophoretic and immunological observations on serum and salivary proteins of man. Arch Oral Biol. 1968 Mar;13(3):257–270. doi: 10.1016/0003-9969(68)90125-8. [DOI] [PubMed] [Google Scholar]
  7. Geiger R., Stuckstedte U., Fritz H. Isolation and characterization of human urinary kallikrein. Hoppe Seylers Z Physiol Chem. 1980 Jul;361(7):1003–1016. doi: 10.1515/bchm2.1980.361.2.1003. [DOI] [PubMed] [Google Scholar]
  8. Grimaldi M. G., Preda A., Scanni A., Dell'Aquila F. Scialografia, tasso di callicreina salivare e scialobiopsia in corso di malattia reumatoide. Reumatismo. 1971 Sep-Oct;23(5):252–256. [PubMed] [Google Scholar]
  9. Herzberg S. M., White C., Wolf R. O. Characterization of salivary proteins in patients with Sjögren's syndrome. Oral Surg Oral Med Oral Pathol. 1973 Dec;36(6):814–817. doi: 10.1016/0030-4220(73)90332-0. [DOI] [PubMed] [Google Scholar]
  10. Hial V., Diniz C. R., Mares-Guia M. Purification and properties of a human urinary kallikrein (kininogenase). Biochemistry. 1974 Oct 8;13(21):4311–4318. doi: 10.1021/bi00718a011. [DOI] [PubMed] [Google Scholar]
  11. Kerby G. P., Taylor S. M. Salivary kallikrein levels in normal and in rheumatoid individuals. J Lab Clin Med. 1968 Apr;71(4):704–708. [PubMed] [Google Scholar]
  12. Lemon M., Fiedler F., Förg-Brey B., Hirschauer C., Leysath G., Fritz H. The isolation and properties of pig submandibular kallikrein. Biochem J. 1979 Jan 1;177(1):159–168. doi: 10.1042/bj1770159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mairs R. J., Beeley J. A. Partial characterization of abnormal salivary proteins associated with rheumatoid arthritis. Clin Sci (Lond) 1985 Dec;69(6):727–735. doi: 10.1042/cs0690727. [DOI] [PubMed] [Google Scholar]
  14. Matsuda Y., Miyazaki K., Moriya H., Fujimoto Y. Human renal kallirein: purification and some properties. Chem Pharm Bull (Tokyo) 1981 Jul;29(7):2106–2108. doi: 10.1248/cpb.29.2106. [DOI] [PubMed] [Google Scholar]
  15. Matthews R. W., Bhoola K. D., Rasker J. J., Jayson M. I. Salivary secretion and connective tissue disease in man. Ann Rheum Dis. 1985 Jan;44(1):20–26. doi: 10.1136/ard.44.1.20. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morrissey J. H. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981 Nov 1;117(2):307–310. doi: 10.1016/0003-2697(81)90783-1. [DOI] [PubMed] [Google Scholar]
  17. Moutsopoulos H. M., Chused T. M., Mann D. L., Klippel J. H., Fauci A. S., Frank M. M., Lawley T. J., Hamburger M. I. Sjögren's syndrome (Sicca syndrome): current issues. Ann Intern Med. 1980 Feb;92(2 Pt 1):212–226. doi: 10.7326/0003-4819-92-2-212. [DOI] [PubMed] [Google Scholar]
  18. Oppenheim F. G., Hay D. I., Franzblau C. Proline-rich proteins from human parotid saliva. I. Isolation and partial characterization. Biochemistry. 1971 Nov;10(23):4233–4238. doi: 10.1021/bi00799a013. [DOI] [PubMed] [Google Scholar]
  19. Preliminary criteria for the classification of systemic sclerosis (scleroderma). Bull Rheum Dis. 1981;31(1):1–6. [PubMed] [Google Scholar]
  20. Shine J., Mason A. J., Evans B. A., Richards R. I. The kallikrein multigene family: specific processing of biologically active peptides. Cold Spring Harb Symp Quant Biol. 1983;48(Pt 1):419–426. doi: 10.1101/sqb.1983.048.01.046. [DOI] [PubMed] [Google Scholar]
  21. Strand V., Talal N. Advances in the diagnosis and concept of Sjögren's syndrome (autoimmune exocrinopathy). Bull Rheum Dis. 1979;30(9):1046–1052. [PubMed] [Google Scholar]
  22. Vío C. P., Figueroa C. D. Subcellular localization of renal kallikrein by ultrastructural immunocytochemistry. Kidney Int. 1985 Jul;28(1):36–42. doi: 10.1038/ki.1985.115. [DOI] [PubMed] [Google Scholar]
  23. van Leeuwen B. H., Grinblat S. M., Johnston C. I. Tissue-specific control of glandular kallikreins. Am J Physiol. 1984 Nov;247(5 Pt 2):F760–F764. doi: 10.1152/ajprenal.1984.247.5.F760. [DOI] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES