Skip to main content
PMC home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1990 May;49(5):310–315. doi: 10.1136/ard.49.5.310

Detection of enterovirus specific RNA sequences in muscle biopsy specimens from patients with adult onset myositis.

G E Yousef 1, D A Isenberg 1, J F Mowbray 1
PMCID: PMC1004075  PMID: 2160802

Abstract

A subgenomic cDNA probe with broad specificity for a range of enteroviruses was used to test by an in situ hybridisation technique for the presence of enterovirus specific genomic sequences in muscle biopsy samples obtained from patients with chronic adult myositis. Virus specific RNA sequences were detected in 6/13 (46%) patients with idiopathic polymyositis or dermatomyositis. Control samples obtained from an equal number of patients suffering from other muscle disorders were negative. A monoclonal antibody specific for an enterovirus group was used to probe for viral antigens by indirect immunoperoxidase staining; all biopsy samples from test and control groups were negative.

Full text

PDF
310

Images in this article

312Image
on p.312
312Image
on p.312
312Image
on p.312
313Image
on p.313
313Image
on p.313

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Bassat M., Machtey I. Picornavirus-like structures in acute dermatomyositis. Am J Clin Pathol. 1972 Sep;58(3):245–249. doi: 10.1093/ajcp/58.3.245. [DOI] [PubMed] [Google Scholar]
  2. Bohan A., Peter J. B., Bowman R. L., Pearson C. M. Computer-assisted analysis of 153 patients with polymyositis and dermatomyositis. Medicine (Baltimore) 1977 Jul;56(4):255–286. doi: 10.1097/00005792-197707000-00001. [DOI] [PubMed] [Google Scholar]
  3. Bohan A., Peter J. B. Polymyositis and dermatomyositis (first of two parts). N Engl J Med. 1975 Feb 13;292(7):344–347. doi: 10.1056/NEJM197502132920706. [DOI] [PubMed] [Google Scholar]
  4. Bowles N. E., Dubowitz V., Sewry C. A., Archard L. C. Dermatomyositis, polymyositis, and Coxsackie-B-virus infection. Lancet. 1987 May 2;1(8540):1004–1007. doi: 10.1016/s0140-6736(87)92271-9. [DOI] [PubMed] [Google Scholar]
  5. Cambridge G. Aetiology and pathogenesis of inflammatory muscle disease. Clin Exp Rheumatol. 1984 Jul-Sep;2(3):263–269. [PubMed] [Google Scholar]
  6. Chou S. M., Gutmann L. Picornavirus-like crystals in subacute polymyositis. Neurology. 1970 Mar;20(3):205–213. doi: 10.1212/wnl.20.3.205. [DOI] [PubMed] [Google Scholar]
  7. Christensen M. L., Pachman L. M., Schneiderman R., Patel D. C., Friedman J. M. Prevalence of Coxsackie B virus antibodies in patients with juvenile dermatomyositis. Arthritis Rheum. 1986 Nov;29(11):1365–1370. doi: 10.1002/art.1780291109. [DOI] [PubMed] [Google Scholar]
  8. DALLDORF G. Neuropathogenicity of group A Coxsackie viruses. J Exp Med. 1957 Jul 1;106(1):69–76. doi: 10.1084/jem.106.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dawkins R. L., Mastaglia F. L. Cell-mediated cytotoxicity to muscle in polymyositis. Effect of immunosuppression. N Engl J Med. 1973 Mar 1;288(9):434–438. doi: 10.1056/NEJM197303012880903. [DOI] [PubMed] [Google Scholar]
  10. Heyderman E. Immunoperoxidase technique in histopathology: applications, methods, and controls. J Clin Pathol. 1979 Oct;32(10):971–978. doi: 10.1136/jcp.32.10.971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Huber S. A., Lodge P. A. Coxsackievirus B-3 myocarditis in Balb/c mice. Evidence for autoimmunity to myocyte antigens. Am J Pathol. 1984 Jul;116(1):21–29. [PMC free article] [PubMed] [Google Scholar]
  12. Isenberg D. A., Rowe D., Shearer M., Novick D., Beverley P. C. Localization of interferons and interleukin 2 in polymyositis and muscular dystrophy. Clin Exp Immunol. 1986 Feb;63(2):450–458. [PMC free article] [PubMed] [Google Scholar]
  13. Kitamura N., Semler B. L., Rothberg P. G., Larsen G. R., Adler C. J., Dorner A. J., Emini E. A., Hanecak R., Lee J. J., van der Werf S. Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature. 1981 Jun 18;291(5816):547–553. doi: 10.1038/291547a0. [DOI] [PubMed] [Google Scholar]
  14. Mastaglia F. L., Walton J. N. Coxsackie virus-like particles in skeletal muscle from a case of polymyositis. J Neurol Sci. 1970 Dec;11(6):593–599. doi: 10.1016/0022-510x(70)90109-7. [DOI] [PubMed] [Google Scholar]
  15. Melnick J. L. Portraits of viruses: the picornaviruses. Intervirology. 1983;20(2-3):61–100. doi: 10.1159/000149376. [DOI] [PubMed] [Google Scholar]
  16. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  17. SUSSMAN M. L., STRAUSS L., HODES H. L. Fatal coxsackie group B virus infection in the newborn; report of a case with necropsy findings and brief review of the literature. AMA J Dis Child. 1959 Apr;97(4):483–492. doi: 10.1001/archpedi.1959.02070010485014. [DOI] [PubMed] [Google Scholar]
  18. Schiraldi O., Iandolo E. Polymyositis accompanying coxsackie virus B2 infection. Infection. 1978;6(1):32–34. doi: 10.1007/BF01641089. [DOI] [PubMed] [Google Scholar]
  19. Strongwater S. L., Dorovini-Zis K., Ball R. D., Schnitzer T. J. A murine model of polymyositis induced by coxsackievirus B1 (Tucson strain). Arthritis Rheum. 1984 Apr;27(4):433–442. doi: 10.1002/art.1780270411. [DOI] [PubMed] [Google Scholar]
  20. Tang T. T., Sedmak G. V., Siegesmund K. A., McCreadie S. R. Chronic myopathy associated with coxsackievirus type A9. A combined electron microscopical and viral isolation study. N Engl J Med. 1975 Mar 20;292(12):608–611. doi: 10.1056/NEJM197503202921203. [DOI] [PubMed] [Google Scholar]
  21. Tracy S. A comparison of genomic homologies among the coxsackievirus B group: use of fragments of the cloned coxsackievirus B3 genome as probes. J Gen Virol. 1984 Dec;65(Pt 12):2167–2172. doi: 10.1099/0022-1317-65-12-2167. [DOI] [PubMed] [Google Scholar]
  22. Travers R. L., Hughes G. R., Cambridge G., Sewell J. R. Coxsackie B neutralisation titres in polymyositis/dermatomyositis. Lancet. 1977 Jun 11;1(8024):1268–1268. doi: 10.1016/s0140-6736(77)92487-4. [DOI] [PubMed] [Google Scholar]
  23. Wilson F. M., Miranda Q. R., Chason J. L., Lerner A. M. Residual pathologic changes following murine coxsackie A and B myocarditis. Am J Pathol. 1969 May;55(2):253–265. [PMC free article] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES