Skip to main content
NCBI home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1990 Aug;49(8):594–597. doi: 10.1136/ard.49.8.594

Cytidine deaminase activity in synovial fluid of patients with rheumatoid arthritis: relation to lactoferrin, acidosis, and cartilage proteoglycan release.

B Månsson 1, P Geborek 1, T Saxne 1, S Björnsson 1
PMCID: PMC1004171  PMID: 2396864

Abstract

It is claimed that cytidine deaminase activity reflects local granulocyte turnover or activity in the synovial fluid of patients with rheumatoid arthritis, but cytidine deaminase is not a granulocyte specific enzyme. Lactoferrin is a granulocyte specific protein that is released from the secondary granulae during activation. We measured cytidine deaminase activity and lactoferrin concentrations in 33 rheumatic synovial fluid samples. Cytidine deaminase activity and lactoferrin concentrations correlated closely, indicating that both analyses reflect similar events in the joint-that is, result in their release from granulocytes. Cytidine deaminase activity and granulocyte concentrations correlated less closely, suggesting that there are additional factors besides the cell number which contribute to this release. Joint acidosis may be one such factor, as pH and cytidine deaminase activity correlated inversely. There was no association with synovial fluid proteoglycan concentrations, a marker of cartilage degradation.

Full text

PDF
594

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adeyemi E. O., Hull R. G., Chadwick V. S., Hughes G. R., Hodgson H. J. Circulating human leucocyte elastase in rheumatoid arthritis. Rheumatol Int. 1986;6(2):57–60. doi: 10.1007/BF00541505. [DOI] [PubMed] [Google Scholar]
  2. Brahn E., Scoville C. D. Biochemical markers of disease activity. Baillieres Clin Rheumatol. 1988 Apr;2(1):153–183. doi: 10.1016/s0950-3579(88)80008-6. [DOI] [PubMed] [Google Scholar]
  3. Edwards S. W., Hughes V., Barlow J., Bucknall R. Immunological detection of myeloperoxidase in synovial fluid from patients with rheumatoid arthritis. Biochem J. 1988 Feb 15;250(1):81–85. doi: 10.1042/bj2500081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Farr M., Kendall M. J., Shuttleworth R., Meynell M. J., Hawkins C. F. Source and significance of 5-nucleotidase in synovial fluid. Ann Rheum Dis. 1973 Jul;32(4):326–330. doi: 10.1136/ard.32.4.326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hansen N. E., Malmquist J., Thorell J. Plasma myeloperoxidase and lactoferrin measured by radioimmunoassay: relations to neutrophil kinetics. Acta Med Scand. 1975 Dec;198(6):437–443. doi: 10.1111/j.0954-6820.1975.tb19572.x. [DOI] [PubMed] [Google Scholar]
  6. Hällgren R., Bjelle A., Venge P. Eosinophil cationic protein in inflammatory synovial effusions as evidence of eosinophil involvement. Ann Rheum Dis. 1984 Aug;43(4):556–562. doi: 10.1136/ard.43.4.556. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones D. D., Bahijri S., Roberts E. L., Williams G. F. Activity of serum cytidine deaminase during pregnancy. Br J Obstet Gynaecol. 1982 Apr;89(4):314–317. doi: 10.1111/j.1471-0528.1982.tb04703.x. [DOI] [PubMed] [Google Scholar]
  8. Jones D. D., Roberts E. L., Williams G. F. Serum cytidine deaminase assay--some pitfalls. Br J Obstet Gynaecol. 1986 Apr;93(4):407–408. doi: 10.1111/j.1471-0528.1986.tb07918.x. [DOI] [PubMed] [Google Scholar]
  9. Malmquist J., Thorell J. I., Wolheim F. A. Lactoferrin and lysozyme in arthritic exudates. Acta Med Scand. 1977;202(4):313–318. doi: 10.1111/j.0954-6820.1977.tb16834.x. [DOI] [PubMed] [Google Scholar]
  10. Pruzanski W., Saito S., Ogryzlo M. A. The significance of lysozyme (muramidase) in rheumatoid arthritis. I. Levels in serum and synovial fluid. Arthritis Rheum. 1970 Jul-Aug;13(4):389–399. doi: 10.1002/art.1780130405. [DOI] [PubMed] [Google Scholar]
  11. Saxne T., Heinegård D., Wollheim F. A. Cartilage proteoglycans in synovial fluid and serum in patients with inflammatory joint disease. Relation to systemic treatment. Arthritis Rheum. 1987 Sep;30(9):972–979. doi: 10.1002/art.1780300903. [DOI] [PubMed] [Google Scholar]
  12. Saxne T., Heinegård D., Wollheim F. A. Therapeutic effects on cartilage metabolism in arthritis as measured by release of proteoglycan structures into the synovial fluid. Ann Rheum Dis. 1986 Jun;45(6):491–497. doi: 10.1136/ard.45.6.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Thompson P. W., Jones D. D., Currey H. L. Cytidine deaminase activity as a measure of acute inflammation in rheumatoid arthritis. Ann Rheum Dis. 1986 Jan;45(1):9–14. doi: 10.1136/ard.45.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thompson P. W. Laboratory markers of joint inflammation and damage. Br J Rheumatol. 1987 Apr;26(2):83–85. doi: 10.1093/rheumatology/26.2.83. [DOI] [PubMed] [Google Scholar]
  15. Yuksel H., Akoğlu T. F. Serum and synovial fluid adenosine deaminase activity in patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis. Ann Rheum Dis. 1988 Jun;47(6):492–495. doi: 10.1136/ard.47.6.492. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES