Abstract
Neutrophil and monocyte activation by immune complexes containing IgE from serum and synovial fluid of patients with rheumatoid arthritis is reported. Activation of the inflammatory cells was measured by stimulation of the respiratory burst with production of intracellular hydrogen peroxide. Generation of hydrogen peroxide was analysed by a flow cytometric method, using the fluorochrome dichlorofluorescein. The technique was modified to allow measurement of cell activation of both neutrophils and monocytes by immune complexes in suspension. Ten of 14 polyethylene glycol precipitates from serum of patients with rheumatoid arthritis and 10/16 synovial fluids of these patients could activate neutrophils. A positive relation was found between the activation of neutrophils and the total concentration of immune complexes, the presence of IgG, and the presence of IgE in the immune complexes. Activation of monocytes was also shown, but to a lesser extent (8/14 rheumatoid serum samples and 8/16 rheumatoid synovial fluids activated monocytes). There was a weak correlation between the concentration of IgE immune complexes and the intensity of fluorescence measured in the monocytes.
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- Babior B. M., Kipnes R. S., Curnutte J. T. Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent. J Clin Invest. 1973 Mar;52(3):741–744. doi: 10.1172/JCI107236. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bass D. A., Parce J. W., Dechatelet L. R., Szejda P., Seeds M. C., Thomas M. Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J Immunol. 1983 Apr;130(4):1910–1917. [PubMed] [Google Scholar]
- De Clerck L. S., Struyf N. J., Bridts C. H., Francx L., Van Offel J. F., Empsten F. A., Westedt M. L., Breedveld F. C., Cats A., Stevens W. J. Humoral immunity and composition of immune complexes in patients with rheumatoid arthritis, with special reference to IgE-containing immune complexes. Clin Exp Rheumatol. 1989 Sep-Oct;7(5):485–492. [PubMed] [Google Scholar]
- De Clerck L. S., Westedt M. L., Cats A., Vermeer B. J., Weltevreden E. F., Bridts C. H., Stevens W. J. IgE deposition in normal skin of patients with rheumatoid arthritis in relation to clinical and laboratory findings. Ann Rheum Dis. 1985 Nov;44(11):772–777. doi: 10.1136/ard.44.11.772. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duque R. E., Ward P. A. Quantitative assessment of neutrophil function by flow cytometry. Anal Quant Cytol Histol. 1987 Mar;9(1):42–48. [PubMed] [Google Scholar]
- Meretey K., Falus A., Erhardt C. C., Maini R. N. IgE and IgE-rheumatoid factors in circulating immune complexes in rheumatoid arthritis. Ann Rheum Dis. 1982 Aug;41(4):405–408. doi: 10.1136/ard.41.4.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Merétey K., Falus A., Böhm U., Permin H., Wiik A. IgE class immune complexes in Felty's syndrome: characterisation of antibody activities in isolated complexes. Ann Rheum Dis. 1984 Apr;43(2):246–250. doi: 10.1136/ard.43.2.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmid I., Schmid P., Giorgi J. V. Conversion of logarithmic channel numbers into relative linear fluorescence intensity. Cytometry. 1988 Nov;9(6):533–538. doi: 10.1002/cyto.990090605. [DOI] [PubMed] [Google Scholar]
- Spiegelberg H. L., Melewicz F. M. Fc receptors specific for IgE on subpopulations of human lymphocytes and monocytes. Clin Immunol Immunopathol. 1980 Mar;15(3):424–433. doi: 10.1016/0090-1229(80)90054-9. [DOI] [PubMed] [Google Scholar]
- Stevens W. J., Bridts C. A method for rapid determination of IgG containing circulating immune complexes using polyethyleneglycol and radioactively labeled protein A. Immunol Lett. 1981 Apr;3(1):1–4. doi: 10.1016/0165-2478(81)90086-9. [DOI] [PubMed] [Google Scholar]