. 2023 Mar 11;11(3):855. doi: 10.3390/biomedicines11030855

Table A1.

List of qPCR-Primers. The primers used for the normalization are highlighted in gray.

Gene	Gene Name	Primer	Sequence	Primer Length [bp]	Product Length [bp]	Efficiency [%]	Source	Primer Design	Primer Concen-tration	R²
*Hif1a*	Hypoxia inducible factor 1 subunit alpha	Forward	TCAAGCAGCAGGAATTGGAAC	21	181	95	NCBI Primer- BLAST	Exon junction	400 nM	0.99
*Hif1a*	Hypoxia inducible factor 1 subunit alpha	Reverse	CTCATCCATTGACTGCCCCA	20	181	95	NCBI Primer- BLAST	Exon junction	400 nM	0.99
*s100b*	S100 calcium binding protein B	Forward	GGTGACAAGCACAAGCTGAAG	21	91	104	NCBI Primer- BLAST	Exon junction	400 nM	0.97
*s100b*	S100 calcium binding protein B	Reverse	CTTCCTGCTCCTTGATTTCCTCCA	24	91	104	NCBI Primer- BLAST	Exon junction	400 nM	0.97
*eno2*	Enolase 2	Forward	AGGTGGATCTCTATACTGCCAAA	23	98	96	PrimerBank ID14290500a1	Intron Inclusion	200 nM	0.99
*eno2*	Enolase 2	Reverse	GTCCCCATCCCTTAGTTCCAG	21	98	96	PrimerBank ID14290500a1	Intron Inclusion	200 nM	0.99
*ICAM-1*	Intercellular adhesion	Forward	GTGGGTCGAAGGTGGTTCTT	20	188	105	NCBI Primer- BLAST	Exon junction	400 nM	1
*ICAM-1*	molecule 1	Reverse	CCGAGGACCATACAGCACG	19	188	105	NCBI Primer- BLAST	Exon junction	400 nM	1
*VCAM-1*	Vascular cell adhesion molecule 1	Forward	CAAAAAGGGACGATTCCGGC	20	187	123	NCBI Primer- BLAST	Intron Inclusion	400 nM	0.96
*VCAM-1*	Vascular cell adhesion molecule 1	Reverse	GTTTCGGGCACATTTCCACA	20	187	123	NCBI Primer- BLAST	Intron Inclusion	400 nM	0.96
*Nrf2*	Nuclear factor, erythroid 2 like 2	Forward	AGCAGGACATGGAGCAAGTT	20	96	117	NCBI Primer- BLAST	Intron Inclusion	400 nM	0.98
*Nrf2*	Nuclear factor, erythroid 2 like 2	Reverse	CAGCGGTAGTATCAGCCAGC	20	96	117	NCBI Primer- BLAST	Intron Inclusion	400 nM	0.98
*Tnf*	Tumor necrosis factor	Forward	CCTGTAGCCCACGTCGTAG	19	148	120	PrimerBank ID133892368c3	Intron Inclusion	500 nM	0.98
*Tnf*	Tumor necrosis factor	Reverse	GGGAGTAGACAAGGTACAACCC	22	148	120	PrimerBank ID133892368c3	Intron Inclusion	500 nM	0.98
Il1β	Interleukin 1 beta	Forward	ATGAAAGACGGCACACCCAC	20	175	129	NCBI Primer- BLAST	Intron Inclusion	400 nM	0.99
Il1β	Interleukin 1 beta	Reverse	GCTTGTGCTCTGCTTGTGAG	20	175	129	NCBI Primer- BLAST	Intron Inclusion	400 nM	0.99
*Il6*	Interleukin 6	Forward	GAGGATACCACTCCCAACAGACC	23	141	106	Aachen 078	Intron Inclusion	400 nM	0.99
*Il6*	Interleukin 6	Reverse	AAGTGCATCATCGTTGTTCATACA	24	141	106	Aachen 078	Intron Inclusion	400 nM	0.99
*Tlr2*	Toll like receptor 2	Forward	ACCTGAGAATGATGTGGGCG	20	202	103	NCBI Primer- BLAST	Non-Exon-junction	400 nM	0.99
*Tlr2*	Toll like receptor 2	Reverse	CATTTGCCCGGAACGAAGTC	20	202	103	NCBI Primer- BLAST	Non-Exon-junction	400 nM	0.99
*Ppia*	Peptidylprolyl isomerase A	Forward	GGCAAATGCTGGACCAAAC	19	110	98	Sundaram et al., PLoS one 2019.	Intron Inclusion	400 nM	0.99
*Ppia*	Peptidylprolyl isomerase A	Reverse	CATTCCTGGACCCAAAACG	19	110	98	Sundaram et al., PLoS one 2019.	Intron Inclusion	400 nM	0.99
s7	Ribosomal protein S7	Forward	AAAGTTCAGTGGCAAGCACG	20	99	99	NCBI Primer- BLAST	Intron Inclusion	400 nM	0.99
s7	Ribosomal protein S7	Reverse	CTGGGGCGCTTCTGCTTATT	20	99	99	NCBI Primer- BLAST	Intron Inclusion	400 nM	0.99