Abstract
Cellular immunity to cartilage proteoglycans may be responsible for sustaining chronic inflammation in ankylosing spondylitis. This hypothesis was examined by measuring peripheral blood and synovial fluid mononuclear cell proliferation in five preparations of human cartilage proteoglycan monomer in vitro. Peripheral blood mononuclear cells from 25 patients and synovial fluid mononuclear cells from five patients were compared with those from normal and disease control subjects matched for age. No significant differences were found between the three groups. This suggests that autoimmune responses to cartilage proteoglycans are unlikely to play a significant part in the pathogenesis of ankylosing spondylitis.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andriopoulos N. A., Mestecky J., Miller E. J., Bradley E. L. Antibodies to native and denatured collagens in sera of patients with rheumatoid arthritis. Arthritis Rheum. 1976 May-Jun;19(3):613–617. doi: 10.1002/art.1780190314. [DOI] [PubMed] [Google Scholar]
- Boniface R. J., Cain P. R., Evans C. H. Articular responses to purified cartilage proteoglycans. Arthritis Rheum. 1988 Feb;31(2):258–266. doi: 10.1002/art.1780310214. [DOI] [PubMed] [Google Scholar]
- Glant T., Csongor J., Szücs T. Immunopathologic role of proteoglycan antigens in rheumatoid joint disease. Scand J Immunol. 1980;11(3):247–252. doi: 10.1111/j.1365-3083.1980.tb00232.x. [DOI] [PubMed] [Google Scholar]
- Golds E. E., Stephen I. B., Esdaile J. M., Strawczynski H., Poole A. R. Lymphocyte transformation to connective tissue antigens in adult and juvenile rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, systemic lupus erythematosus, and a nonarthritic control population. Cell Immunol. 1983 Nov;82(1):196–209. doi: 10.1016/0008-8749(83)90153-3. [DOI] [PubMed] [Google Scholar]
- Heinegård D., Björne-Persson A., Cöster L., Franzén A., Gardell S., Malmström A., Paulsson M., Sandfalk R., Vogel K. The core proteins of large and small interstitial proteoglycans from various connective tissues form distinct subgroups. Biochem J. 1985 Aug 15;230(1):181–194. doi: 10.1042/bj2300181. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heinegård D., Sommarin Y. Isolation and characterization of proteoglycans. Methods Enzymol. 1987;144:319–372. doi: 10.1016/0076-6879(87)44186-4. [DOI] [PubMed] [Google Scholar]
- Henderson B., Pettipher E. R., Murphy G. Metalloproteinases and cartilage proteoglycan depletion in chronic arthritis. Comparison of antigen-induced and polycation-induced arthritis. Arthritis Rheum. 1990 Feb;33(2):241–246. doi: 10.1002/art.1780330213. [DOI] [PubMed] [Google Scholar]
- Kuijer R., van de Stadt R. J., van Kampen G. P., de Koning M. H., van de Voorde-Vissers E., van der Korst J. K. Heterogeneity of proteoglycans extracted before and after collagenase treatment of human articular cartilage. II. Variations in composition with age and tissue source. Arthritis Rheum. 1986 Oct;29(10):1248–1255. doi: 10.1002/art.1780291010. [DOI] [PubMed] [Google Scholar]
- Mikecz K., Glant T. T., Baron M., Poole A. R. Isolation of proteoglycan-specific T lymphocytes from patients with ankylosing spondylitis. Cell Immunol. 1988 Mar;112(1):55–63. doi: 10.1016/0008-8749(88)90275-4. [DOI] [PubMed] [Google Scholar]
- Ofosu-Appiah W. A., Warrington R. J., Wilkins J. A. Interleukin 2 responsive T cell clones from rheumatoid and normal subjects: proliferative responses to connective tissue elements. Clin Immunol Immunopathol. 1989 Feb;50(2):264–271. doi: 10.1016/0090-1229(89)90134-7. [DOI] [PubMed] [Google Scholar]
- Rajapakse D. A., Bywaters E. G. Cell-mediated immunity to cartilage proteoglycan in relapsing polychondritis. Clin Exp Immunol. 1974 Mar;16(3):497–502. [PMC free article] [PubMed] [Google Scholar]
- Roughley P. J., White R. J. Age-related changes in the structure of the proteoglycan subunits from human articular cartilage. J Biol Chem. 1980 Jan 10;255(1):217–224. [PubMed] [Google Scholar]
- Saxne T., Heinegård D., Wollheim F. A. Cartilage proteoglycans in synovial fluid and serum in patients with inflammatory joint disease. Relation to systemic treatment. Arthritis Rheum. 1987 Sep;30(9):972–979. doi: 10.1002/art.1780300903. [DOI] [PubMed] [Google Scholar]
- Sigal L. H., Johnston S. L., Phillips P. E. Cellular immune responses to cartilage components in rheumatoid arthritis and osteoarthritis: a review and report of a study. Clin Exp Rheumatol. 1988 Jan-Mar;6(1):59–66. [PubMed] [Google Scholar]
- Trentham D. E., Dynesius R. A., Rocklin R. E., David J. R. Cellular sensitivity to collagen in rheumatoid arthritis. N Engl J Med. 1978 Aug 17;299(7):327–332. doi: 10.1056/NEJM197808172990703. [DOI] [PubMed] [Google Scholar]
- Van Vollenhoven R. F., Soriano A., McCarthy P. E., Schwartz R. L., Garbrecht F. C., Thorbecke G. J., Siskind G. W. The role of immunity to cartilage proteoglycan in adjuvant arthritis. Intravenous injection of bovine proteoglycan enhances adjuvant arthritis. J Immunol. 1988 Aug 15;141(4):1168–1173. [PubMed] [Google Scholar]
- Wieslander J., Heinegård D. Immunochemical analysis of cartilage proteoglycans. Antigenic determinants of substructures. Biochem J. 1979 Apr 1;179(1):35–45. doi: 10.1042/bj1790035. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Eden W., Holoshitz J., Nevo Z., Frenkel A., Klajman A., Cohen I. R. Arthritis induced by a T-lymphocyte clone that responds to Mycobacterium tuberculosis and to cartilage proteoglycans. Proc Natl Acad Sci U S A. 1985 Aug;82(15):5117–5120. doi: 10.1073/pnas.82.15.5117. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van der Linden S., Valkenburg H. A., Cats A. Evaluation of diagnostic criteria for ankylosing spondylitis. A proposal for modification of the New York criteria. Arthritis Rheum. 1984 Apr;27(4):361–368. doi: 10.1002/art.1780270401. [DOI] [PubMed] [Google Scholar]