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. 1993 May;52(5):354–359. doi: 10.1136/ard.52.5.354

Receptor expression in synovial fluid neutrophils from patients with rheumatoid arthritis.

F Watson 1, J J Robinson 1, M Phelan 1, R C Bucknall 1, S W Edwards 1
PMCID: PMC1005049  PMID: 8100700

Abstract

OBJECTIVES--The aim of this study was to determine if neutrophils isolated from the blood and synovial fluid of patients with rheumatoid arthritis had patterns of receptor expression resembling those of blood neutrophils from controls which had been activated and primed in vitro. METHODS--Fluorescence activated cell sorting was used to measure receptor expression in paired blood and synovial fluid neutrophils from patients and in control neutrophils exposed to phorbol myristate acetate and granulocyte-macrophage colony stimulating factor. RESULTS--There was no significant difference in the patterns of receptor expression in blood neutrophils from patients and healthy controls, but neutrophils in the synovial fluid had been primed and activated within the joint. About 50% of rheumatoid synovial fluid neutrophil samples expressed Fc gamma RI, a high affinity receptor for monomeric IgG, which is only expressed in neutrophils exposed to cytokines. CONCLUSIONS--Synovial fluid neutrophils are activated and primed within the inflamed joint and hence their ability to respond to activating factors such as immune complexes will be modulated. As the expression of Fc gamma RI requires active biosynthesis, this work indicates that selective gene activation occurs when neutrophils are recruited into rheumatoid joints.

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Selected References

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  1. Atkinson Y. H., Marasco W. A., Lopez A. F., Vadas M. A. Recombinant human tumor necrosis factor-alpha. Regulation of N-formylmethionylleucylphenylalanine receptor affinity and function on human neutrophils. J Clin Invest. 1988 Mar;81(3):759–765. doi: 10.1172/JCI113381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baggiolini M., Walz A., Kunkel S. L. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J Clin Invest. 1989 Oct;84(4):1045–1049. doi: 10.1172/JCI114265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berger M., O'Shea J., Cross A. S., Folks T. M., Chused T. M., Brown E. J., Frank M. M. Human neutrophils increase expression of C3bi as well as C3b receptors upon activation. J Clin Invest. 1984 Nov;74(5):1566–1571. doi: 10.1172/JCI111572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berkow R. L., Wang D., Larrick J. W., Dodson R. W., Howard T. H. Enhancement of neutrophil superoxide production by preincubation with recombinant human tumor necrosis factor. J Immunol. 1987 Dec 1;139(11):3783–3791. [PubMed] [Google Scholar]
  5. Dularay B., Elson C. J., Dieppe P. A. Enhanced oxidative response of polymorphonuclear leukocytes from synovial fluids of patients with rheumatoid arthritis. Autoimmunity. 1988;1(3):159–169. doi: 10.3109/08916938808997161. [DOI] [PubMed] [Google Scholar]
  6. Edwards S. W., Holden C. S., Humphreys J. M., Hart C. A. Granulocyte-macrophage colony-stimulating factor (GM-CSF) primes the respiratory burst and stimulates protein biosynthesis in human neutrophils. FEBS Lett. 1989 Oct 9;256(1-2):62–66. doi: 10.1016/0014-5793(89)81718-1. [DOI] [PubMed] [Google Scholar]
  7. Edwards S. W., Say J. E., Hart C. A. Oxygen-dependent killing of Staphylococcus aureus by human neutrophils. J Gen Microbiol. 1987 Dec;133(12):3591–3597. doi: 10.1099/00221287-133-12-3591. [DOI] [PubMed] [Google Scholar]
  8. Edwards S. W., Watson F., MacLeod R., Davies J. Receptor expression and oxidase activity in human neutrophils: regulation by granulocyte-macrophage colony-stimulating factor and dependence upon protein biosynthesis. Biosci Rep. 1990 Aug;10(4):393–401. doi: 10.1007/BF01117239. [DOI] [PubMed] [Google Scholar]
  9. Emery P., Lopez A. F., Burns G. F., Vadas M. A. Synovial fluid neutrophils of patients with rheumatoid arthritis have membrane antigen changes that reflect activation. Ann Rheum Dis. 1988 Jan;47(1):34–39. doi: 10.1136/ard.47.1.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Feldmann M., Brennan F. M., Chantry D., Haworth C., Turner M., Abney E., Buchan G., Barrett K., Barkley D., Chu A. Cytokine production in the rheumatoid joint: implications for treatment. Ann Rheum Dis. 1990 Jun;49 (Suppl 1):480–486. [PubMed] [Google Scholar]
  11. Gale R., Bertouch J. V., Bradley J., Roberts-Thomson P. J. Direct activation of neutrophil chemiluminescence by rheumatoid sera and synovial fluid. Ann Rheum Dis. 1983 Apr;42(2):158–162. doi: 10.1136/ard.42.2.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Huizinga T. W., van der Schoot C. E., Jost C., Klaassen R., Kleijer M., von dem Borne A. E., Roos D., Tetteroo P. A. The PI-linked receptor FcRIII is released on stimulation of neutrophils. Nature. 1988 Jun 16;333(6174):667–669. doi: 10.1038/333667a0. [DOI] [PubMed] [Google Scholar]
  13. Humphreys J. M., Hughes V., Edwards S. W. Stimulation of protein synthesis in human neutrophils by gamma-interferon. Biochem Pharmacol. 1989 Apr 15;38(8):1241–1246. doi: 10.1016/0006-2952(89)90329-8. [DOI] [PubMed] [Google Scholar]
  14. Humphreys J. M., Rugman F. P., Davies J. M., Mimnagh P., Hart C. A., Edwards S. W. Effects of recombinant human granulocyte colony-stimulating factor on neutrophil function in vitro and in vivo following chemotherapy and autologous bone marrow transplantation. J Clin Lab Immunol. 1991 Feb;34(2):55–61. [PubMed] [Google Scholar]
  15. Kuijpers T. W., Tool A. T., van der Schoot C. E., Ginsel L. A., Onderwater J. J., Roos D., Verhoeven A. J. Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation. Blood. 1991 Aug 15;78(4):1105–1111. [PubMed] [Google Scholar]
  16. Lindemann A., Riedel D., Oster W., Meuer S. C., Blohm D., Mertelsmann R. H., Herrmann F. Granulocyte/macrophage colony-stimulating factor induces interleukin 1 production by human polymorphonuclear neutrophils. J Immunol. 1988 Feb 1;140(3):837–839. [PubMed] [Google Scholar]
  17. Lindemann A., Riedel D., Oster W., Ziegler-Heitbrock H. W., Mertelsmann R., Herrmann F. Granulocyte-macrophage colony-stimulating factor induces cytokine secretion by human polymorphonuclear leukocytes. J Clin Invest. 1989 Apr;83(4):1308–1312. doi: 10.1172/JCI114016. [DOI] [PMC free article] [PubMed] [Google Scholar] [Retracted]
  18. Merry P., Winyard P. G., Morris C. J., Grootveld M., Blake D. R. Oxygen free radicals, inflammation, and synovitis: and synovitis: the current status. Ann Rheum Dis. 1989 Oct;48(10):864–870. doi: 10.1136/ard.48.10.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Robinson J. J., Watson F., Bucknall R. C., Edwards S. W. Stimulation of neutrophils by insoluble immunoglobulin aggregates from synovial fluid of patients with rheumatoid arthritis. Eur J Clin Invest. 1992 May;22(5):314–318. doi: 10.1111/j.1365-2362.1992.tb01468.x. [DOI] [PubMed] [Google Scholar]
  20. Robinson J., Watson F., Bucknall R. C., Edwards S. W. Activation of neutrophil reactive-oxidant production by synovial fluid from patients with inflammatory joint disease. Soluble and insoluble immunoglobulin aggregates activate different pathways in primed and unprimed cells. Biochem J. 1992 Sep 1;286(Pt 2):345–351. doi: 10.1042/bj2860345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sandborg R. R., Smolen J. E. Early biochemical events in leukocyte activation. Lab Invest. 1988 Sep;59(3):300–320. [PubMed] [Google Scholar]
  22. Watson F., Robinson J. J., Edwards S. W. Neutrophil function in whole blood and after purification: changes in receptor expression, oxidase activity and responsiveness to cytokines. Biosci Rep. 1992 Apr;12(2):123–133. doi: 10.1007/BF02351217. [DOI] [PubMed] [Google Scholar]
  23. Weisbart R. H., Golde D. W., Clark S. C., Wong G. G., Gasson J. C. Human granulocyte-macrophage colony-stimulating factor is a neutrophil activator. 1985 Mar 28-Apr 3Nature. 314(6009):361–363. doi: 10.1038/314361a0. [DOI] [PubMed] [Google Scholar]
  24. Weisbart R. H., Golde D. W., Gasson J. C. Biosynthetic human GM-CSF modulates the number and affinity of neutrophil f-Met-Leu-Phe receptors. J Immunol. 1986 Dec 1;137(11):3584–3587. [PubMed] [Google Scholar]

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